Molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla R. M. SM.

Jerangau Merah (Boesenbergia stenophylla) is highly endemic to the highland of Borneo. Due to its medicinal properties, it is highly sought which made their population declining. Rapid development and anthropogenic disturbance near the habitat may give negative impact on the Jerangau Merah populatio...

وصف كامل

محفوظ في:

التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Toyat, Aicher Joseph
التنسيق:	أطروحة
اللغة:	English English
منشور في:	2017
الموضوعات:	Micropropagation Molecular characterization Boesenbergia stenophylla
الوصول للمادة أونلاين:	http://psasir.upm.edu.my/id/eprint/83743/1/t%20FSPM%202017%202%20%281800001051%29.pdf
الوسوم:	إضافة وسم لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!

id	my-upm-ir.83743
record_format	uketd_dc
spelling	my-upm-ir.837432022-01-05T01:06:24Z Molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla R. M. SM. 2017-02 Toyat, Aicher Joseph Jerangau Merah (Boesenbergia stenophylla) is highly endemic to the highland of Borneo. Due to its medicinal properties, it is highly sought which made their population declining. Rapid development and anthropogenic disturbance near the habitat may give negative impact on the Jerangau Merah population in their natural habitat. To date, there are no conservation strategies prepared for this species. Therefore a study was conducted to develop strategies to conserve this species which includes DNA profiling, DNA barcoding, micropropagation and nursery planting. A total of three natural locations of B. stenophylla were found in Bario, Ba’kelalan and Long Semadoh forest. Based on the DNA profiling with RAPD and SSRs markers there was no genetic variations found among sub populations from Bario. This finding implied that no variations may be caused to low hybridization because flowering is rare and propagation through rhizome is more dominant. DNA analysis using CpDNA and NrDNA markers showed that this species clades under the Borneo-Boesenbergia section. This proves further that this species is endemic and could not be found outside of Borneo. A micropropagation protocol was achieved for the in vitro cultures of B. stenophylla. It includes initial culturing using shoot tips in MS media containing 0.2 mg/L NAA for shoot induction followed by sub-culturing to MS media with 2 mg/L BAP + 0.4 mg/L NAA for rapid shoot elongation. B. stenophylla is highly endemic, having small populations, possibly lacks genetic variations and difficult to grow in in vitro cultures. The nursery studies show that the plant has it unique sympodial growth that proved that cannot be confined in the small polybag, so bedded planting was suggested for improving the growth in the nursery conditions. Therefore, this study suggests that their conservation should remain as in situ, seedlings production under optimum nursery conditions should be carried out near to their natural populations and further monitoring of new population occurrence for documentations. Micropropagation Molecular characterization Boesenbergia stenophylla 2017-02 Thesis http://psasir.upm.edu.my/id/eprint/83743/ http://psasir.upm.edu.my/id/eprint/83743/1/t%20FSPM%202017%202%20%281800001051%29.pdf text en public masters Universiti Putra Malaysia Micropropagation Molecular characterization Boesenbergia stenophylla Abdullah, Nur Ashikin Psyquay English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Abdullah, Nur Ashikin Psyquay
topic	Micropropagation Molecular characterization Boesenbergia stenophylla
spellingShingle	Micropropagation Molecular characterization Boesenbergia stenophylla Toyat, Aicher Joseph Molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla R. M. SM.
description	Jerangau Merah (Boesenbergia stenophylla) is highly endemic to the highland of Borneo. Due to its medicinal properties, it is highly sought which made their population declining. Rapid development and anthropogenic disturbance near the habitat may give negative impact on the Jerangau Merah population in their natural habitat. To date, there are no conservation strategies prepared for this species. Therefore a study was conducted to develop strategies to conserve this species which includes DNA profiling, DNA barcoding, micropropagation and nursery planting. A total of three natural locations of B. stenophylla were found in Bario, Ba’kelalan and Long Semadoh forest. Based on the DNA profiling with RAPD and SSRs markers there was no genetic variations found among sub populations from Bario. This finding implied that no variations may be caused to low hybridization because flowering is rare and propagation through rhizome is more dominant. DNA analysis using CpDNA and NrDNA markers showed that this species clades under the Borneo-Boesenbergia section. This proves further that this species is endemic and could not be found outside of Borneo. A micropropagation protocol was achieved for the in vitro cultures of B. stenophylla. It includes initial culturing using shoot tips in MS media containing 0.2 mg/L NAA for shoot induction followed by sub-culturing to MS media with 2 mg/L BAP + 0.4 mg/L NAA for rapid shoot elongation. B. stenophylla is highly endemic, having small populations, possibly lacks genetic variations and difficult to grow in in vitro cultures. The nursery studies show that the plant has it unique sympodial growth that proved that cannot be confined in the small polybag, so bedded planting was suggested for improving the growth in the nursery conditions. Therefore, this study suggests that their conservation should remain as in situ, seedlings production under optimum nursery conditions should be carried out near to their natural populations and further monitoring of new population occurrence for documentations.
format	Thesis
qualification_level	Master's degree
author	Toyat, Aicher Joseph
author_facet	Toyat, Aicher Joseph
author_sort	Toyat, Aicher Joseph
title	Molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla R. M. SM.
title_short	Molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla R. M. SM.
title_full	Molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla R. M. SM.
title_fullStr	Molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla R. M. SM.
title_full_unstemmed	Molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla R. M. SM.
title_sort	molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla r. m. sm.
granting_institution	Universiti Putra Malaysia
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/83743/1/t%20FSPM%202017%202%20%281800001051%29.pdf
_version_	1747813416151547904

Molecular and micropropagation approaches for ex-situ conservation of boesenbergia stenophylla R. M. SM.

مواد مشابهة