Prevalence,Isolation Techniques, Antibiotic Pattern and Plasmid Profile of Salmonella in Broiler Chicken During Production, Processing and Retail Outlets

Five hundred and forty nine (549) of carcasses and intestinals content and 73 samples of chicken litter and feed were examined for Salmonella. Two hundred and thirty (237) Salmonella isolates belonging to 15 different serotypes were isolated. The predominant serotypes were: S. enteritidis (35.2%)...

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Main Author:	Hashim, Jamal Khair
Format:	Thesis
Language:	English English
Published:	1998
Subjects:	Salmonella infections in animals Broilers (Chickens) - Diseases
Online Access:	http://psasir.upm.edu.my/id/eprint/8387/1/FSMB_1998_4_A.pdf
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spelling	my-upm-ir.83872012-11-01T07:43:30Z Prevalence,Isolation Techniques, Antibiotic Pattern and Plasmid Profile of Salmonella in Broiler Chicken During Production, Processing and Retail Outlets 1998 Hashim, Jamal Khair Five hundred and forty nine (549) of carcasses and intestinals content and 73 samples of chicken litter and feed were examined for Salmonella. Two hundred and thirty (237) Salmonella isolates belonging to 15 different serotypes were isolated. The predominant serotypes were: S. enteritidis (35.2%), S. muenchen (20%), S. kentucky (14.3%), S. blockley (10.4%) and S. chincol (5.2%). S. enteritidis were detected throughout the broiler production chain. The detection rate of Salmonella in poultry carcasses under different conditions were as follows: 83% (126/232), direct enrichment of pelleted (DEP) rinse fluid In Rapaport Vasiliadis broth (RV) incubated at 42°C; 28% (43/232) ,for both DEP enriched in RV and Manitol Selanite Cystine Broth (MSCB) incubated at 37°C; and 9% (14/232), streaking the pellet directly onto the plating media. In a parallel analysis, the conventional method detected Salmonella in 43.5% (115/264) of carcasses, litter and feed samples compared to 18.2% (48/264) detected by rapid method TECRA UNIQUE Salmonella test kit. Two hundrad and thirty seven (237) isolates of Salmonella belonging to 15 different serovars were susceptible to gentamicin, advosin and enrofloxacin except for one isolate of S. agona, which was resistant to enrofloxacin. S. kentucky isolates displayed 11 discrete antibiotic resistant patterns, followed by S. blockley (7), s. agona (4) and S. muenchen (3). Plasmids were detected in 80% of the isolates with molecular weights ranging from <1 to 50 MDa. The frequency of plasmids in different serovars are as follows: S. blockely (96%), S. chnicol (92%), s. muenchen (90%); S. kentuckey (88%) and S. enteritidis (75%). Different variations in plasmid profile pattern were exhibited by S. muenchen (24 patterns), S. kentucky (11 patterns), S. blockley (14 patterns), S. enteritidis (7 patterns), S. chincol (4 patterns) and S. newport (5 patterns). Salmonella infections in animals Broilers (Chickens) - Diseases 1998 Thesis http://psasir.upm.edu.my/id/eprint/8387/ http://psasir.upm.edu.my/id/eprint/8387/1/FSMB_1998_4_A.pdf application/pdf en public masters Universiti Putra Malaysia Salmonella infections in animals Broilers (Chickens) - Diseases Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Salmonella infections in animals Broilers (Chickens) - Diseases
spellingShingle	Salmonella infections in animals Broilers (Chickens) - Diseases Hashim, Jamal Khair Prevalence,Isolation Techniques, Antibiotic Pattern and Plasmid Profile of Salmonella in Broiler Chicken During Production, Processing and Retail Outlets
description	Five hundred and forty nine (549) of carcasses and intestinals content and 73 samples of chicken litter and feed were examined for Salmonella. Two hundred and thirty (237) Salmonella isolates belonging to 15 different serotypes were isolated. The predominant serotypes were: S. enteritidis (35.2%), S. muenchen (20%), S. kentucky (14.3%), S. blockley (10.4%) and S. chincol (5.2%). S. enteritidis were detected throughout the broiler production chain. The detection rate of Salmonella in poultry carcasses under different conditions were as follows: 83% (126/232), direct enrichment of pelleted (DEP) rinse fluid In Rapaport Vasiliadis broth (RV) incubated at 42°C; 28% (43/232) ,for both DEP enriched in RV and Manitol Selanite Cystine Broth (MSCB) incubated at 37°C; and 9% (14/232), streaking the pellet directly onto the plating media. In a parallel analysis, the conventional method detected Salmonella in 43.5% (115/264) of carcasses, litter and feed samples compared to 18.2% (48/264) detected by rapid method TECRA UNIQUE Salmonella test kit. Two hundrad and thirty seven (237) isolates of Salmonella belonging to 15 different serovars were susceptible to gentamicin, advosin and enrofloxacin except for one isolate of S. agona, which was resistant to enrofloxacin. S. kentucky isolates displayed 11 discrete antibiotic resistant patterns, followed by S. blockley (7), s. agona (4) and S. muenchen (3). Plasmids were detected in 80% of the isolates with molecular weights ranging from <1 to 50 MDa. The frequency of plasmids in different serovars are as follows: S. blockely (96%), S. chnicol (92%), s. muenchen (90%); S. kentuckey (88%) and S. enteritidis (75%). Different variations in plasmid profile pattern were exhibited by S. muenchen (24 patterns), S. kentucky (11 patterns), S. blockley (14 patterns), S. enteritidis (7 patterns), S. chincol (4 patterns) and S. newport (5 patterns).
format	Thesis
qualification_level	Master's degree
author	Hashim, Jamal Khair
author_facet	Hashim, Jamal Khair
author_sort	Hashim, Jamal Khair
title	Prevalence,Isolation Techniques, Antibiotic Pattern and Plasmid Profile of Salmonella in Broiler Chicken During Production, Processing and Retail Outlets
title_short	Prevalence,Isolation Techniques, Antibiotic Pattern and Plasmid Profile of Salmonella in Broiler Chicken During Production, Processing and Retail Outlets
title_full	Prevalence,Isolation Techniques, Antibiotic Pattern and Plasmid Profile of Salmonella in Broiler Chicken During Production, Processing and Retail Outlets
title_fullStr	Prevalence,Isolation Techniques, Antibiotic Pattern and Plasmid Profile of Salmonella in Broiler Chicken During Production, Processing and Retail Outlets
title_full_unstemmed	Prevalence,Isolation Techniques, Antibiotic Pattern and Plasmid Profile of Salmonella in Broiler Chicken During Production, Processing and Retail Outlets
title_sort	prevalence,isolation techniques, antibiotic pattern and plasmid profile of salmonella in broiler chicken during production, processing and retail outlets
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	1998
url	http://psasir.upm.edu.my/id/eprint/8387/1/FSMB_1998_4_A.pdf
_version_	1747810794682187776

Prevalence,Isolation Techniques, Antibiotic Pattern and Plasmid Profile of Salmonella in Broiler Chicken During Production, Processing and Retail Outlets

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