Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor)

This study was undertaken to characterise the biological activities of Garcinia atroviridis, a food plant and traditional vegetable, as well as to isolate and identify its bioactive constituents Ethnobotanical reports on the use of G atroviridis as an anti-infective agent and biopreservative, co...

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Main Author:	Mohamed Mackeen, Muhammad Mukram
Format:	Thesis
Language:	English English
Published:	1998
Subjects:	Bioactive compounds Garcinia Biological assay
Online Access:	http://psasir.upm.edu.my/id/eprint/8393/1/FSMB_1998_13_A.pdf
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id	my-upm-ir.8393
record_format	uketd_dc
spelling	my-upm-ir.83932012-10-31T01:51:08Z Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor) 1998 Mohamed Mackeen, Muhammad Mukram This study was undertaken to characterise the biological activities of Garcinia atroviridis, a food plant and traditional vegetable, as well as to isolate and identify its bioactive constituents Ethnobotanical reports on the use of G atroviridis as an anti-infective agent and biopreservative, coupled with the paucity of scientific reports on the biological activities of G atroviridis were the basis for selecting this plant as the subject of this study Crude extracts (methanol dimethyl sulfmade-91) of various parts of this plant were screened for antimicrobial (disc diffusion method), Cytotoxic (microtitration method), brine shrimp toxic, antitumour-promoting (Epstem Barr Virus activation assay) and antioxidant (ferric thiocyanate and thiobarbitunc assays) activities. The crude extracts exhibited predominantly antibacterial activity with the roots showing the strongest inhibition against the test bacteria at the minimum inhibitory dose (MID) of 156µg/disc. Although all the extracts failed to inhibit the growth of most of the test fungi, significant antifungal activity against Cladosporium herbarum was exhibited by most notably the fruit extract (MID 100 µg), and the leaf extract (MID 400 µg) None of the extracts were Significantly cytotoxic and lethal towards brine shrimps. The root, leaf, trunk and stem bark extracts (except for the fruits) showed strong antioxidant activity exceeding that of the standard antioxidant, α-tocopherol. Antitumour promoting activity (> 95% inhibition) was shown by the fruit, leaf, stem and trunk bark extracts. Bioactive compounds Garcinia Biological assay 1998 Thesis http://psasir.upm.edu.my/id/eprint/8393/ http://psasir.upm.edu.my/id/eprint/8393/1/FSMB_1998_13_A.pdf application/pdf en public masters Universiti Putra Malaysia Bioactive compounds Garcinia Biological assay Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Bioactive compounds Garcinia Biological assay
spellingShingle	Bioactive compounds Garcinia Biological assay Mohamed Mackeen, Muhammad Mukram Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor)
description	This study was undertaken to characterise the biological activities of Garcinia atroviridis, a food plant and traditional vegetable, as well as to isolate and identify its bioactive constituents Ethnobotanical reports on the use of G atroviridis as an anti-infective agent and biopreservative, coupled with the paucity of scientific reports on the biological activities of G atroviridis were the basis for selecting this plant as the subject of this study Crude extracts (methanol dimethyl sulfmade-91) of various parts of this plant were screened for antimicrobial (disc diffusion method), Cytotoxic (microtitration method), brine shrimp toxic, antitumour-promoting (Epstem Barr Virus activation assay) and antioxidant (ferric thiocyanate and thiobarbitunc assays) activities. The crude extracts exhibited predominantly antibacterial activity with the roots showing the strongest inhibition against the test bacteria at the minimum inhibitory dose (MID) of 156µg/disc. Although all the extracts failed to inhibit the growth of most of the test fungi, significant antifungal activity against Cladosporium herbarum was exhibited by most notably the fruit extract (MID 100 µg), and the leaf extract (MID 400 µg) None of the extracts were Significantly cytotoxic and lethal towards brine shrimps. The root, leaf, trunk and stem bark extracts (except for the fruits) showed strong antioxidant activity exceeding that of the standard antioxidant, α-tocopherol. Antitumour promoting activity (> 95% inhibition) was shown by the fruit, leaf, stem and trunk bark extracts.
format	Thesis
qualification_level	Master's degree
author	Mohamed Mackeen, Muhammad Mukram
author_facet	Mohamed Mackeen, Muhammad Mukram
author_sort	Mohamed Mackeen, Muhammad Mukram
title	Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor)
title_short	Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor)
title_full	Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor)
title_fullStr	Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor)
title_full_unstemmed	Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor)
title_sort	bioassay-guided isolation, and identification of bioactive compounds from garcinia atroviridis (asam gelugor)
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	1998
url	http://psasir.upm.edu.my/id/eprint/8393/1/FSMB_1998_13_A.pdf
_version_	1747810796157534208

Bioassay-Guided Isolation, and Identification of Bioactive Compounds from Garcinia Atroviridis (Asam Gelugor)

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