Partial Characterisation and Some Properties of a Thermophilic Bacterium Isolated from a Petroleum Reservoir in Sarawak, Malaysia

The hypertbermophilic bacteria (MRU5) was isolated from an oil-producing well in Sarawak. Malaysia. MRU5 is strictly anaerobic and was grown in enrichment media which were been degassed with CO2:N2 (80:20). By Gram and spore staining, MRU5 was found to be a gram negative, non-spore producing bact...

Full description

Saved in:

Bibliographic Details
Main Author:	You, Li Ling
Format:	Thesis
Language:	English English
Published:	1999
Subjects:	Thermophilic bacteria - Sarawak.
Online Access:	http://psasir.upm.edu.my/id/eprint/8404/1/FSMB_1999_7_A.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.8404
record_format	uketd_dc
spelling	my-upm-ir.84042012-07-03T02:49:47Z Partial Characterisation and Some Properties of a Thermophilic Bacterium Isolated from a Petroleum Reservoir in Sarawak, Malaysia 1999 You, Li Ling The hypertbermophilic bacteria (MRU5) was isolated from an oil-producing well in Sarawak. Malaysia. MRU5 is strictly anaerobic and was grown in enrichment media which were been degassed with CO2:N2 (80:20). By Gram and spore staining, MRU5 was found to be a gram negative, non-spore producing bacteria. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) revealed the shape of the bacteria to be irregular and coccoid which occurs singly, in pairs or clumps. The bacteria measures 0.75µm in diameter and is surrounded by an outer sheath coating. MRU5 was found to grow best at pH 7 and 10% salinity with the optimal temperature range of between 90°C to 100°C. MRU5 was grown in xylose, glucose, fructose, arabinose, lactose, and sucrose. From the results obtained, xylose was the most efficiently utilised fermentable carbon source. Growth of the microorganism is supported by yeast extract. End products such as organic acids and H2S were produced by fermentation of carbon sources but none of the tested solvents were produced. This microorganism is able to reduce oil viscosity by producing organic acids. This means that MRU5 has the potential to be ecologically important for enhancing oil recovery. The modified Gompertz equation was found to be appropriate to model the growth of bacterium MRU5 at different medium compositions and culture conditions. Thermophilic bacteria - Sarawak. 1999 Thesis http://psasir.upm.edu.my/id/eprint/8404/ http://psasir.upm.edu.my/id/eprint/8404/1/FSMB_1999_7_A.pdf application/pdf en public masters Universiti Putra Malaysia Thermophilic bacteria - Sarawak. Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Thermophilic bacteria - Sarawak.
spellingShingle	Thermophilic bacteria - Sarawak. You, Li Ling Partial Characterisation and Some Properties of a Thermophilic Bacterium Isolated from a Petroleum Reservoir in Sarawak, Malaysia
description	The hypertbermophilic bacteria (MRU5) was isolated from an oil-producing well in Sarawak. Malaysia. MRU5 is strictly anaerobic and was grown in enrichment media which were been degassed with CO2:N2 (80:20). By Gram and spore staining, MRU5 was found to be a gram negative, non-spore producing bacteria. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) revealed the shape of the bacteria to be irregular and coccoid which occurs singly, in pairs or clumps. The bacteria measures 0.75µm in diameter and is surrounded by an outer sheath coating. MRU5 was found to grow best at pH 7 and 10% salinity with the optimal temperature range of between 90°C to 100°C. MRU5 was grown in xylose, glucose, fructose, arabinose, lactose, and sucrose. From the results obtained, xylose was the most efficiently utilised fermentable carbon source. Growth of the microorganism is supported by yeast extract. End products such as organic acids and H2S were produced by fermentation of carbon sources but none of the tested solvents were produced. This microorganism is able to reduce oil viscosity by producing organic acids. This means that MRU5 has the potential to be ecologically important for enhancing oil recovery. The modified Gompertz equation was found to be appropriate to model the growth of bacterium MRU5 at different medium compositions and culture conditions.
format	Thesis
qualification_level	Master's degree
author	You, Li Ling
author_facet	You, Li Ling
author_sort	You, Li Ling
title	Partial Characterisation and Some Properties of a Thermophilic Bacterium Isolated from a Petroleum Reservoir in Sarawak, Malaysia
title_short	Partial Characterisation and Some Properties of a Thermophilic Bacterium Isolated from a Petroleum Reservoir in Sarawak, Malaysia
title_full	Partial Characterisation and Some Properties of a Thermophilic Bacterium Isolated from a Petroleum Reservoir in Sarawak, Malaysia
title_fullStr	Partial Characterisation and Some Properties of a Thermophilic Bacterium Isolated from a Petroleum Reservoir in Sarawak, Malaysia
title_full_unstemmed	Partial Characterisation and Some Properties of a Thermophilic Bacterium Isolated from a Petroleum Reservoir in Sarawak, Malaysia
title_sort	partial characterisation and some properties of a thermophilic bacterium isolated from a petroleum reservoir in sarawak, malaysia
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	1999
url	http://psasir.upm.edu.my/id/eprint/8404/1/FSMB_1999_7_A.pdf
_version_	1747810798858665984

Partial Characterisation and Some Properties of a Thermophilic Bacterium Isolated from a Petroleum Reservoir in Sarawak, Malaysia

Similar Items