Pathogenicity of banana blood disease bacterium HR2 Mardi and characterization of its virulent proteins

In Malaysia, bacterial wilt has been identified as one of the main constraints for banana production. Blood Disease Bacterium (BDB) is a gram negative bacteria that caused Banana Blood Disease (BBD) which identified as one of the most important pathogen of ban...

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Main Author:	Bakar, Ros Azrinawati Hana
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Bananas - Diseases and pests - Malaysia
Online Access:	http://psasir.upm.edu.my/id/eprint/84066/1/FP%202018%20100%20-%20ir.pdf
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spelling	my-upm-ir.840662022-01-04T04:25:20Z Pathogenicity of banana blood disease bacterium HR2 Mardi and characterization of its virulent proteins 2018-07 Bakar, Ros Azrinawati Hana In Malaysia, bacterial wilt has been identified as one of the main constraints for banana production. Blood Disease Bacterium (BDB) is a gram negative bacteria that caused Banana Blood Disease (BBD) which identified as one of the most important pathogen of banana (Musa sp.) which could infect almost all cultivated banana varieties. On the other hand, BDB is grouped into Phylotype IV which placed in a subgroup of the R. solanacearum species complex. BDB was reported to be pathogenic to a single host, banana. Hence, the first objectives of this study is to determine the host range and symptomatology study of BDB on selected hosts. Results from this study showed that BDB only pathogenic to banana not to other tested hosts such as tomato (Solanum lycopersicum) and heliconia (Heliconia sp.) and confirmed to be virulence and able to infect banana. The disease symptoms were wilting of leaves, discoloration of vascular tissues and eventually leads to plant death. The second objectives of this study is to identify and quantify effector protein(s) of BDB in selected media. From this study, findings indicated that 17 potential virulent proteins were obtained from virulence inducing media (M63). Prediction of virulence characteristics shows that the only five proteins were found to be effector proteins of BDB. The proteins were Signal peptidase, Lipoprotein, Uncharacterized protein, Membrane protein and Serine protease. In conclusion, BDB was confirmed to be a single host-pathogen to banana (Musa sp.) plant, and confirmed to be virulence for the proteomics study and based on proteomics analysis revealed five virulent proteins, namely Signal peptidase, Lipoprotein, Uncharacterized protein, Membrane protein and Serine protease associated with the virulence of BDB. Bananas - Diseases and pests - Malaysia 2018-07 Thesis http://psasir.upm.edu.my/id/eprint/84066/ http://psasir.upm.edu.my/id/eprint/84066/1/FP%202018%20100%20-%20ir.pdf text en public masters Universiti Putra Malaysia Bananas - Diseases and pests - Malaysia Ahmad, Khairulmazmi
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Ahmad, Khairulmazmi
topic	Bananas - Diseases and pests - Malaysia
spellingShingle	Bananas - Diseases and pests - Malaysia Bakar, Ros Azrinawati Hana Pathogenicity of banana blood disease bacterium HR2 Mardi and characterization of its virulent proteins
description	In Malaysia, bacterial wilt has been identified as one of the main constraints for banana production. Blood Disease Bacterium (BDB) is a gram negative bacteria that caused Banana Blood Disease (BBD) which identified as one of the most important pathogen of banana (Musa sp.) which could infect almost all cultivated banana varieties. On the other hand, BDB is grouped into Phylotype IV which placed in a subgroup of the R. solanacearum species complex. BDB was reported to be pathogenic to a single host, banana. Hence, the first objectives of this study is to determine the host range and symptomatology study of BDB on selected hosts. Results from this study showed that BDB only pathogenic to banana not to other tested hosts such as tomato (Solanum lycopersicum) and heliconia (Heliconia sp.) and confirmed to be virulence and able to infect banana. The disease symptoms were wilting of leaves, discoloration of vascular tissues and eventually leads to plant death. The second objectives of this study is to identify and quantify effector protein(s) of BDB in selected media. From this study, findings indicated that 17 potential virulent proteins were obtained from virulence inducing media (M63). Prediction of virulence characteristics shows that the only five proteins were found to be effector proteins of BDB. The proteins were Signal peptidase, Lipoprotein, Uncharacterized protein, Membrane protein and Serine protease. In conclusion, BDB was confirmed to be a single host-pathogen to banana (Musa sp.) plant, and confirmed to be virulence for the proteomics study and based on proteomics analysis revealed five virulent proteins, namely Signal peptidase, Lipoprotein, Uncharacterized protein, Membrane protein and Serine protease associated with the virulence of BDB.
format	Thesis
qualification_level	Master's degree
author	Bakar, Ros Azrinawati Hana
author_facet	Bakar, Ros Azrinawati Hana
author_sort	Bakar, Ros Azrinawati Hana
title	Pathogenicity of banana blood disease bacterium HR2 Mardi and characterization of its virulent proteins
title_short	Pathogenicity of banana blood disease bacterium HR2 Mardi and characterization of its virulent proteins
title_full	Pathogenicity of banana blood disease bacterium HR2 Mardi and characterization of its virulent proteins
title_fullStr	Pathogenicity of banana blood disease bacterium HR2 Mardi and characterization of its virulent proteins
title_full_unstemmed	Pathogenicity of banana blood disease bacterium HR2 Mardi and characterization of its virulent proteins
title_sort	pathogenicity of banana blood disease bacterium hr2 mardi and characterization of its virulent proteins
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/84066/1/FP%202018%20100%20-%20ir.pdf
_version_	1747813433768673280

Pathogenicity of banana blood disease bacterium HR2 Mardi and characterization of its virulent proteins

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