Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia

Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA were used to study strains of Vibrio vulnificus and Vibrio parahaemolyticus isolated from cockles (Anadara granosa). 36 strains of V vuln(ficus isolates were examined. The prevalent biotype was biotype 1 (72.2...

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Main Author:	Hussein, Nasreldin Elhadi
Format:	Thesis
Language:	English English
Published:	1999
Subjects:	Vibrio vulnificus - Malaysia. Vibrio parahaemolyticus - Malaysia.
Online Access:	http://psasir.upm.edu.my/id/eprint/8411/1/FSMB_1999_15_A.pdf
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id	my-upm-ir.8411
record_format	uketd_dc
spelling	my-upm-ir.84112012-07-03T04:42:58Z Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia 1999 Hussein, Nasreldin Elhadi Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA were used to study strains of Vibrio vulnificus and Vibrio parahaemolyticus isolated from cockles (Anadara granosa). 36 strains of V vuln(ficus isolates were examined. The prevalent biotype was biotype 1 (72.2% of the isolates) and 2 (27.8%). Twenty one strains of biotypes 1 and 2 harboured plasmid DNA ranging in size from 1.4 to 9.7 megaDalton. No particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. Two primers demonstrated polymorphisms in all strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability among both biotypes 1 and 2 of the V vulnificus strains investigated. RAPD identity across biotypes was also observed among the V vulnificus strains. 35 Vibrio parahaemolyticus Kanagawa-negative strains were isolated.· Twenty six strains of V parahaemolyticus were carried small plasmid(s) of 1.3 to 9.7 MegaDalton that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The RAPD fingerprinting using three primers demonstrated polymorphisms in all thirty-five strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD profiles revealed a high level of DNA sequence diversity within the V. parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. Vibrio vulnificus - Malaysia. Vibrio parahaemolyticus - Malaysia. 1999 Thesis http://psasir.upm.edu.my/id/eprint/8411/ http://psasir.upm.edu.my/id/eprint/8411/1/FSMB_1999_15_A.pdf application/pdf en public masters Universiti Putra Malaysia Vibrio vulnificus - Malaysia. Vibrio parahaemolyticus - Malaysia. Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Vibrio vulnificus - Malaysia. Vibrio parahaemolyticus - Malaysia.
spellingShingle	Vibrio vulnificus - Malaysia. Vibrio parahaemolyticus - Malaysia. Hussein, Nasreldin Elhadi Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
description	Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA were used to study strains of Vibrio vulnificus and Vibrio parahaemolyticus isolated from cockles (Anadara granosa). 36 strains of V vuln(ficus isolates were examined. The prevalent biotype was biotype 1 (72.2% of the isolates) and 2 (27.8%). Twenty one strains of biotypes 1 and 2 harboured plasmid DNA ranging in size from 1.4 to 9.7 megaDalton. No particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. Two primers demonstrated polymorphisms in all strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability among both biotypes 1 and 2 of the V vulnificus strains investigated. RAPD identity across biotypes was also observed among the V vulnificus strains. 35 Vibrio parahaemolyticus Kanagawa-negative strains were isolated.· Twenty six strains of V parahaemolyticus were carried small plasmid(s) of 1.3 to 9.7 MegaDalton that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The RAPD fingerprinting using three primers demonstrated polymorphisms in all thirty-five strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD profiles revealed a high level of DNA sequence diversity within the V. parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus.
format	Thesis
qualification_level	Master's degree
author	Hussein, Nasreldin Elhadi
author_facet	Hussein, Nasreldin Elhadi
author_sort	Hussein, Nasreldin Elhadi
title	Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_short	Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_full	Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_fullStr	Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_full_unstemmed	Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia
title_sort	isolation and molecular characterisation of vibrio vulnificus and vibrio parahaemolyticus from cockles (anadara granosa) in malaysia
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	1999
url	http://psasir.upm.edu.my/id/eprint/8411/1/FSMB_1999_15_A.pdf
_version_	1747810800567844864

Isolation and Molecular Characterisation of Vibrio Vulnificus and Vibrio Parahaemolyticus from Cockles (Anadara Granosa) in Malaysia

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