Isolation and Characterisation of CDNA Clones Encoding Adp-Glucose Pyrophosphorylase (AGP) from Sago Palm (Metroxylon Sagu)

In general, the biosynthetic steps required for starch biosynthesis involves three major enzymes: ADP-glucose pyrophosphorylase (AGP), starch synthase (SS) and starch branching enzyme (SBE). Many studies have indicated that AGP is an important control point of flux through the pathway of starch b...

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Main Author:	Au, Sian Loong
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Swamp sago Biosynthesis Starch - Synthesis
Online Access:	http://psasir.upm.edu.my/id/eprint/8435/1/FSMB_2001_8_A.pdf
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id	my-upm-ir.8435
record_format	uketd_dc
spelling	my-upm-ir.84352012-10-31T03:31:26Z Isolation and Characterisation of CDNA Clones Encoding Adp-Glucose Pyrophosphorylase (AGP) from Sago Palm (Metroxylon Sagu) 2001 Au, Sian Loong In general, the biosynthetic steps required for starch biosynthesis involves three major enzymes: ADP-glucose pyrophosphorylase (AGP), starch synthase (SS) and starch branching enzyme (SBE). Many studies have indicated that AGP is an important control point of flux through the pathway of starch biosynthesis in many plant species. All plant AGPs are composed of two subunit types, large (lAGP) and small (sAGP) subunits that give rise to an U2(:l2 heterotetrameric native enzyme. The presence of both subunits are crucial for the stability and activity of the enzyme. One full length and three partial AGP cDNA clones have been isolated and clBracterised from sago palm leaves and pith by a PCR amplification technique. Three of the clones (agp1l9, agpp10 and agpp12) encode AGP large subunits; the fourth clone (agpll) encodes a small subunit. The complete cDNA of agpl2· has been isolated from a mature leaf cDNA library by a PCR screening technique. Semi-quantitative RT-PCR analysis revealed that agpl19 was leaf specific while agppl0 and agpp12 were pith specific. agpll was found to be present in leaves as well as pith tissue. Swamp sago Biosynthesis Starch - Synthesis 2001 Thesis http://psasir.upm.edu.my/id/eprint/8435/ http://psasir.upm.edu.my/id/eprint/8435/1/FSMB_2001_8_A.pdf application/pdf en public masters Universiti Putra Malaysia Swamp sago Biosynthesis Starch - Synthesis Food Science and Technology English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Swamp sago Biosynthesis Starch - Synthesis
spellingShingle	Swamp sago Biosynthesis Starch - Synthesis Au, Sian Loong Isolation and Characterisation of CDNA Clones Encoding Adp-Glucose Pyrophosphorylase (AGP) from Sago Palm (Metroxylon Sagu)
description	In general, the biosynthetic steps required for starch biosynthesis involves three major enzymes: ADP-glucose pyrophosphorylase (AGP), starch synthase (SS) and starch branching enzyme (SBE). Many studies have indicated that AGP is an important control point of flux through the pathway of starch biosynthesis in many plant species. All plant AGPs are composed of two subunit types, large (lAGP) and small (sAGP) subunits that give rise to an U2(:l2 heterotetrameric native enzyme. The presence of both subunits are crucial for the stability and activity of the enzyme. One full length and three partial AGP cDNA clones have been isolated and clBracterised from sago palm leaves and pith by a PCR amplification technique. Three of the clones (agp1l9, agpp10 and agpp12) encode AGP large subunits; the fourth clone (agpll) encodes a small subunit. The complete cDNA of agpl2· has been isolated from a mature leaf cDNA library by a PCR screening technique. Semi-quantitative RT-PCR analysis revealed that agpl19 was leaf specific while agppl0 and agpp12 were pith specific. agpll was found to be present in leaves as well as pith tissue.
format	Thesis
qualification_level	Master's degree
author	Au, Sian Loong
author_facet	Au, Sian Loong
author_sort	Au, Sian Loong
title	Isolation and Characterisation of CDNA Clones Encoding Adp-Glucose Pyrophosphorylase (AGP) from Sago Palm (Metroxylon Sagu)
title_short	Isolation and Characterisation of CDNA Clones Encoding Adp-Glucose Pyrophosphorylase (AGP) from Sago Palm (Metroxylon Sagu)
title_full	Isolation and Characterisation of CDNA Clones Encoding Adp-Glucose Pyrophosphorylase (AGP) from Sago Palm (Metroxylon Sagu)
title_fullStr	Isolation and Characterisation of CDNA Clones Encoding Adp-Glucose Pyrophosphorylase (AGP) from Sago Palm (Metroxylon Sagu)
title_full_unstemmed	Isolation and Characterisation of CDNA Clones Encoding Adp-Glucose Pyrophosphorylase (AGP) from Sago Palm (Metroxylon Sagu)
title_sort	isolation and characterisation of cdna clones encoding adp-glucose pyrophosphorylase (agp) from sago palm (metroxylon sagu)
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/8435/1/FSMB_2001_8_A.pdf
_version_	1747810806458744832

Isolation and Characterisation of CDNA Clones Encoding Adp-Glucose Pyrophosphorylase (AGP) from Sago Palm (Metroxylon Sagu)

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