Antioxidant and anti-diabetic properties, and phytochemical profile of different parts of Mangifera odorata Griff. fruit

Mangifera odorata G. or known as “kuini” in Malay represent the hybrid form between M. indica (mango) and M. foetida (bacang). Mango and its fruit wastes are known for its high nutritive and pharmaceutical value, however there was a lack of scientific data focusing on the nutritional composition and...

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Main Author:	Lasano, Nur Fatimah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Antioxidants Diabetes Mangifera
Online Access:	http://psasir.upm.edu.my/id/eprint/84442/1/FSTM%202019%2031%20ir.pdf
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spelling	my-upm-ir.844422022-01-03T08:12:41Z Antioxidant and anti-diabetic properties, and phytochemical profile of different parts of Mangifera odorata Griff. fruit 2019-04 Lasano, Nur Fatimah Mangifera odorata G. or known as “kuini” in Malay represent the hybrid form between M. indica (mango) and M. foetida (bacang). Mango and its fruit wastes are known for its high nutritive and pharmaceutical value, however there was a lack of scientific data focusing on the nutritional composition and biological activities of M. odorata fruit. In this study, peel, pulp and seed kernel of M. odorata fruit were investigated for their nutritional composition. Then, the samples were freeze-dried and extracted using several extraction solvents (acetone, ethanol, methanol at 60% v/v and water), and their effect on antioxidants (TPC, TFC, FRAP assay, and DPPH assay) and anti-diabetic activities (α-amylase and α-glucosidase inhibition assay) were determined. Next, the ethanolic extract of peel, pulp, and seed kernel from M. odorata was further analysed for their phytochemical profile using ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Ortbitrap-MS/MS). The results indicated that the seed kernel is rich in fat, protein, carbohydrate, and ash (2.76, 2.62, 43.31 and 1.29 g/100 g fresh fruit, respectively). Meanwhile, the peel contains significantly higher fibre, potassium, sulfur, aluminum, calcium, manganese, iron, boron, β-carotene and ascorbic acid compared to seed kernel and pulp. Results show that the seed kernel has the highest TPC and exhibits the strongest scavenging activity compared to other samples. Among the tested solvents, 60% ethanol extract showed highest reducing power in peel and pulp. In addition, acetone at 60% from peel and pulp extracts showed high scavenging activity and TPC in peel and seed kernel. Anti-diabetic assays revealed that the peel, pulp and seed kernel were not active inhibitors of α-amylase and α-glucosidase. The most abundant compounds identified in M. odorata fruit were phenolic acid, ellagic acid, and flavonoid, which mostly found in peel and seed kernel. In conclusion, M. odorata by-products (peel and seed kernel) contained a high nutritional value with good antioxidant properties, hence might be potentially used as a functional food ingredient. Antioxidants Diabetes Mangifera 2019-04 Thesis http://psasir.upm.edu.my/id/eprint/84442/ http://psasir.upm.edu.my/id/eprint/84442/1/FSTM%202019%2031%20ir.pdf text en public masters Universiti Putra Malaysia Antioxidants Diabetes Mangifera Ramli, Nurul Shazini
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Ramli, Nurul Shazini
topic	Antioxidants Diabetes Mangifera
spellingShingle	Antioxidants Diabetes Mangifera Lasano, Nur Fatimah Antioxidant and anti-diabetic properties, and phytochemical profile of different parts of Mangifera odorata Griff. fruit
description	Mangifera odorata G. or known as “kuini” in Malay represent the hybrid form between M. indica (mango) and M. foetida (bacang). Mango and its fruit wastes are known for its high nutritive and pharmaceutical value, however there was a lack of scientific data focusing on the nutritional composition and biological activities of M. odorata fruit. In this study, peel, pulp and seed kernel of M. odorata fruit were investigated for their nutritional composition. Then, the samples were freeze-dried and extracted using several extraction solvents (acetone, ethanol, methanol at 60% v/v and water), and their effect on antioxidants (TPC, TFC, FRAP assay, and DPPH assay) and anti-diabetic activities (α-amylase and α-glucosidase inhibition assay) were determined. Next, the ethanolic extract of peel, pulp, and seed kernel from M. odorata was further analysed for their phytochemical profile using ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Ortbitrap-MS/MS). The results indicated that the seed kernel is rich in fat, protein, carbohydrate, and ash (2.76, 2.62, 43.31 and 1.29 g/100 g fresh fruit, respectively). Meanwhile, the peel contains significantly higher fibre, potassium, sulfur, aluminum, calcium, manganese, iron, boron, β-carotene and ascorbic acid compared to seed kernel and pulp. Results show that the seed kernel has the highest TPC and exhibits the strongest scavenging activity compared to other samples. Among the tested solvents, 60% ethanol extract showed highest reducing power in peel and pulp. In addition, acetone at 60% from peel and pulp extracts showed high scavenging activity and TPC in peel and seed kernel. Anti-diabetic assays revealed that the peel, pulp and seed kernel were not active inhibitors of α-amylase and α-glucosidase. The most abundant compounds identified in M. odorata fruit were phenolic acid, ellagic acid, and flavonoid, which mostly found in peel and seed kernel. In conclusion, M. odorata by-products (peel and seed kernel) contained a high nutritional value with good antioxidant properties, hence might be potentially used as a functional food ingredient.
format	Thesis
qualification_level	Master's degree
author	Lasano, Nur Fatimah
author_facet	Lasano, Nur Fatimah
author_sort	Lasano, Nur Fatimah
title	Antioxidant and anti-diabetic properties, and phytochemical profile of different parts of Mangifera odorata Griff. fruit
title_short	Antioxidant and anti-diabetic properties, and phytochemical profile of different parts of Mangifera odorata Griff. fruit
title_full	Antioxidant and anti-diabetic properties, and phytochemical profile of different parts of Mangifera odorata Griff. fruit
title_fullStr	Antioxidant and anti-diabetic properties, and phytochemical profile of different parts of Mangifera odorata Griff. fruit
title_full_unstemmed	Antioxidant and anti-diabetic properties, and phytochemical profile of different parts of Mangifera odorata Griff. fruit
title_sort	antioxidant and anti-diabetic properties, and phytochemical profile of different parts of mangifera odorata griff. fruit
granting_institution	Universiti Putra Malaysia
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/84442/1/FSTM%202019%2031%20ir.pdf
_version_	1747813472345784320

Antioxidant and anti-diabetic properties, and phytochemical profile of different parts of Mangifera odorata Griff. fruit

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