The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies

The purpose of this study was to investigate the cholesterol removal by some bifidobacterium spp. at in vitro and in vivo conditions with the emphasis of their bile salt deconjugation ability. Bile salt hydrolase (BSH) activity, which is the measurement of enzyme activity responsible for bile sal...

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Main Author:	Sabet, Maryam Rezaei
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Bifidobacterium Cholesterol
Online Access:	http://psasir.upm.edu.my/id/eprint/8455/1/FSMB_2001_31_IR.pdf
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id	my-upm-ir.8455
record_format	uketd_dc
spelling	my-upm-ir.84552024-01-24T02:12:38Z The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies 2001-07 Sabet, Maryam Rezaei The purpose of this study was to investigate the cholesterol removal by some bifidobacterium spp. at in vitro and in vivo conditions with the emphasis of their bile salt deconjugation ability. Bile salt hydrolase (BSH) activity, which is the measurement of enzyme activity responsible for bile salt deconjugation, was quantified by high pressure liquid chromatography (HPLC) assay. B. in/antis G001204 was the isolate with the highest deconjugation rate in TPY broth supplemented with 5mM GCDC. Generally all the isolates deconjugated glycoconjugated bile acid in higher amount (P<0.05) compared to tauroconjugates. Likewise in overall the percentage of deconjugation activity was higher in TPY medium supplemented with 5mM bile acids (P<0.05) compared to the TPY broth with 10mM bile acids. Cholesterol removal from media was strain-dependent. The percentage of cholesterol assimilated in TPY containing 0.52mM cholesterol plus bile acids ranged from 4.0% for B. infantis F41134 to 47.0% for B. infantis G001204. The presence of bile salt was prerequisite for cholesterol removal.Results of the in vivo experiment showed that total cholesterol concentration in rats fed on the high-cholesterol diet plus either B. in/antis 0001204 or B. animalis ATCC 27672 in a 2-week period were significantly (P<0.05) lower than the control group. Total fecal bile acid excretion increased in animal groups throughout the high-cholesterol diet feeding and probiotic-treated groups had higher excretion rate of fecal bile acids compared to the control significantly (P<0.05). Bifidobacterium Cholesterol 2001-07 Thesis http://psasir.upm.edu.my/id/eprint/8455/ http://psasir.upm.edu.my/id/eprint/8455/1/FSMB_2001_31_IR.pdf text en public doctoral Universiti Putra Malaysia Bifidobacterium Cholesterol Food Science and Technology Abdul Manap, Mohd Yazid English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Abdul Manap, Mohd Yazid
topic	Bifidobacterium Cholesterol
spellingShingle	Bifidobacterium Cholesterol Sabet, Maryam Rezaei The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies
description	The purpose of this study was to investigate the cholesterol removal by some bifidobacterium spp. at in vitro and in vivo conditions with the emphasis of their bile salt deconjugation ability. Bile salt hydrolase (BSH) activity, which is the measurement of enzyme activity responsible for bile salt deconjugation, was quantified by high pressure liquid chromatography (HPLC) assay. B. in/antis G001204 was the isolate with the highest deconjugation rate in TPY broth supplemented with 5mM GCDC. Generally all the isolates deconjugated glycoconjugated bile acid in higher amount (P<0.05) compared to tauroconjugates. Likewise in overall the percentage of deconjugation activity was higher in TPY medium supplemented with 5mM bile acids (P<0.05) compared to the TPY broth with 10mM bile acids. Cholesterol removal from media was strain-dependent. The percentage of cholesterol assimilated in TPY containing 0.52mM cholesterol plus bile acids ranged from 4.0% for B. infantis F41134 to 47.0% for B. infantis G001204. The presence of bile salt was prerequisite for cholesterol removal.Results of the in vivo experiment showed that total cholesterol concentration in rats fed on the high-cholesterol diet plus either B. in/antis 0001204 or B. animalis ATCC 27672 in a 2-week period were significantly (P<0.05) lower than the control group. Total fecal bile acid excretion increased in animal groups throughout the high-cholesterol diet feeding and probiotic-treated groups had higher excretion rate of fecal bile acids compared to the control significantly (P<0.05).
format	Thesis
qualification_level	Doctorate
author	Sabet, Maryam Rezaei
author_facet	Sabet, Maryam Rezaei
author_sort	Sabet, Maryam Rezaei
title	The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies
title_short	The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies
title_full	The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies
title_fullStr	The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies
title_full_unstemmed	The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies
title_sort	role of bifidobacterium spp. on cholestrol assimilation in the in vitro and in vivo studies
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/8455/1/FSMB_2001_31_IR.pdf
_version_	1794018753414955008

The Role of Bifidobacterium SPP. on Cholestrol Assimilation in the in Vitro and in Vivo Studies

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