Development of a Diagnostic Oligonucleotide DNA Probe for the Rapid Detection of Vibrio Cholerae O139

Vibrio cholerae O139 Bengal emerged as the second etiologic agent of cholera in the Indian subcontinent in late 1992, it then spread to several neighboring countries and also some developed countries. V. cholerae O139 Bengal is closely related to V. cholerae O1 E1 Tor strains associated with the...

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Main Author:	Khaw, Aik Kia
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Vibrio cholerae Nucleic acid probes DNA probes
Online Access:	http://psasir.upm.edu.my/id/eprint/8459/1/FSMB_2001_35_IR.pdf
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id	my-upm-ir.8459
record_format	uketd_dc
spelling	my-upm-ir.84592024-01-24T09:13:41Z Development of a Diagnostic Oligonucleotide DNA Probe for the Rapid Detection of Vibrio Cholerae O139 2001-12 Khaw, Aik Kia Vibrio cholerae O139 Bengal emerged as the second etiologic agent of cholera in the Indian subcontinent in late 1992, it then spread to several neighboring countries and also some developed countries. V. cholerae O139 Bengal is closely related to V. cholerae O1 E1 Tor strains associated with the seventh pandemic, and it causes a disease which is virtually indistinguishable from cholera caused by V. cholerae O1. V. cholerae O139 Bengal and V. cholerae O1 E1 Tor share several phenotypic and genotypic properties. However, all the genes of the rfb complex which encode the O antigen in V. cholerae 01 El Tor have been found deleted in V. cholerae O139. In their place, there is a new chromosomal region detected. Based on a published sequence, six set of V. cholerae O139 primers have been designed. Primer combination Sl -AS2 (5'-AGATGCCGAAGACTATAA-3' and 5'-GAGGAATAACAACTGAGA-3') was found to be specific for detection of V. cholerae O139 in a polymerase chain reaction (peR) assay, as they produced an amplicon of 520 bp from all tested pure cultures of V. cholerae O139 strains but not from 39 pure cultures of other bacteria. The newly designed primer combination has been used to develop a, diagnotic kit for the identification of V. cholerae O139 in our laboratory. Vibrio cholerae Nucleic acid probes DNA probes 2001-12 Thesis http://psasir.upm.edu.my/id/eprint/8459/ http://psasir.upm.edu.my/id/eprint/8459/1/FSMB_2001_35_IR.pdf text en public masters Universiti Putra Malaysia Vibrio cholerae Nucleic acid probes DNA probes Food Science and Technology Radu, Son English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Radu, Son
topic	Vibrio cholerae Nucleic acid probes DNA probes
spellingShingle	Vibrio cholerae Nucleic acid probes DNA probes Khaw, Aik Kia Development of a Diagnostic Oligonucleotide DNA Probe for the Rapid Detection of Vibrio Cholerae O139
description	Vibrio cholerae O139 Bengal emerged as the second etiologic agent of cholera in the Indian subcontinent in late 1992, it then spread to several neighboring countries and also some developed countries. V. cholerae O139 Bengal is closely related to V. cholerae O1 E1 Tor strains associated with the seventh pandemic, and it causes a disease which is virtually indistinguishable from cholera caused by V. cholerae O1. V. cholerae O139 Bengal and V. cholerae O1 E1 Tor share several phenotypic and genotypic properties. However, all the genes of the rfb complex which encode the O antigen in V. cholerae 01 El Tor have been found deleted in V. cholerae O139. In their place, there is a new chromosomal region detected. Based on a published sequence, six set of V. cholerae O139 primers have been designed. Primer combination Sl -AS2 (5'-AGATGCCGAAGACTATAA-3' and 5'-GAGGAATAACAACTGAGA-3') was found to be specific for detection of V. cholerae O139 in a polymerase chain reaction (peR) assay, as they produced an amplicon of 520 bp from all tested pure cultures of V. cholerae O139 strains but not from 39 pure cultures of other bacteria. The newly designed primer combination has been used to develop a, diagnotic kit for the identification of V. cholerae O139 in our laboratory.
format	Thesis
qualification_level	Master's degree
author	Khaw, Aik Kia
author_facet	Khaw, Aik Kia
author_sort	Khaw, Aik Kia
title	Development of a Diagnostic Oligonucleotide DNA Probe for the Rapid Detection of Vibrio Cholerae O139
title_short	Development of a Diagnostic Oligonucleotide DNA Probe for the Rapid Detection of Vibrio Cholerae O139
title_full	Development of a Diagnostic Oligonucleotide DNA Probe for the Rapid Detection of Vibrio Cholerae O139
title_fullStr	Development of a Diagnostic Oligonucleotide DNA Probe for the Rapid Detection of Vibrio Cholerae O139
title_full_unstemmed	Development of a Diagnostic Oligonucleotide DNA Probe for the Rapid Detection of Vibrio Cholerae O139
title_sort	development of a diagnostic oligonucleotide dna probe for the rapid detection of vibrio cholerae o139
granting_institution	Universiti Putra Malaysia
granting_department	Food Science and Technology
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/8459/1/FSMB_2001_35_IR.pdf
_version_	1794018754137423872

Development of a Diagnostic Oligonucleotide DNA Probe for the Rapid Detection of Vibrio Cholerae O139

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