Effects of co-loaded doxorubicin and thymoquinone calcium carbonate nanoparticles on MDA MB231 breast cancer stem cells
A subtype of cells, cancer stem cells (CSCs) within the breast cancer has been implicated for the metastasis, chemo/radiotherapy resistance and relapse resulting in poor prognosis. The main objective of the study was to determine the effects of drugs- loaded (thymoquinone, doxo...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/84898/1/IB%202019%2014%20-%20ir.pdf |
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| Summary: | A subtype of cells, cancer stem cells (CSCs) within the breast cancer has been
implicated for the metastasis, chemo/radiotherapy resistance and relapse resulting in poor
prognosis. The main objective of the study was to determine the effects of drugs- loaded
(thymoquinone, doxorubicin, and a combination of thymoquinone and doxorubicin-loaded)
aragonite CaCO3 nanoparticles (ACNP) on breast cancer stem cells. The formulated blank and
drugs-loaded nanoparticles were characterized for physicochemical properties. The
cytotoxic effect of blank and drug-loaded nanoparticles on MDA MB231 breast cancer
cell line, 3D mammosphere, normal breast cells (MDF10A) and normal fibroblast (3T3)
were also analysed. Morphological changes, sphere forming assay, cancer stem cell
self-renewal capacity, ALDH activity analysis, CD44 and CD24 expression were carried out. The
prepared nanoparticles were pleomorphic with sizes ranging from 53.65±10.29nm to
60.49±11.36nm and overall negative charge. The encapsulating efficiency of Dox and TQ in the dual
loaded nanoparticles was found to be 95.8 and 41.6% respectively. The XRD patterns revealed strong
crystallizations in blank and drug loaded formulation, while FTIR showed little alteration upon
loading Dox and TQ. About 100% of drug release was noticed at pH 4.8, 70% at pH 6 while only 50% at
pH 7.4. The blank nanoparticle was biocompatible, cell viability of 80% at a high
concentration of 1000ug/ml. MDA MB231 IC50 dosages of drug-loaded nanoparticle were not toxic to
the normal cells. For monolayer culture, the combination therapy showed enhanced apoptosis,
reduction in cellular migration and invasion when compared to the single drug loaded nanoparticle
and the free drugs. Scanning electron microscopy and transmission electron microscopy
showed presence of cell shrinkage, cell membrane blebbing and disruption of cell
membrane. For cancer stem cell enriched mammosphere, the combination therapy showed
enhanced apoptosis, reduction in ALDH activity and expression of CD44 and CD24 surface maker,
reduction in cancer stem cells metastatic capacity, inhibition of 3D sphere formation and cancer
stem cell self-renewal capacity when compared to the free drugs and the single drug loaded
nanoparticle. Scanning electron microscope showed poor spheroid formation, cell membrane
blebbing, presence of cell shrinkage, distortion in the spheroid architecture. Thus, the cockle
shell-derived aragonite calcium carbonate nanoparticle system provides a simple and efficient
platform for multiple drug delivery and pH sensitive release. The combined drugs-loaded cockle
shell-derived aragonite calcium carbonate nanoparticles (Dox/TQ-ACNP) showed higher efficacy in MDA
MB231 breast cancer cells at lower dose of doxorubicin or thymoquinone, efficiently destroyed the
breast CSCs and may be a potential curative strategy for the management of breast cancer
recurrence and metastasis. |
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