Identification of a mycorrhiza isolate from Paphiopedilum barbatum (Lindl.) pfitzer from Ex situ location and determination of its growth-enhancing capabilities

Orchids and mycorrhiza are known to have a symbiotic relationship that function particularly in seed germination and also in promoting overall plant growth. It is believed that a certain orchid mycorrhiza that functions to enhance plant growth also lives symbiotically in the roots of Paphiopedilum b...

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Main Author:	Idris, Nurul Aliaa
Format:	Thesis
Language:	English
Published:	2010
Subjects:	Mycorrhizas Crops - Growth Paphiopedilum
Online Access:	http://psasir.upm.edu.my/id/eprint/85130/1/IB%202010%205%20ir.pdf
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id	my-upm-ir.85130
record_format	uketd_dc
spelling	my-upm-ir.851302021-12-30T07:40:59Z Identification of a mycorrhiza isolate from Paphiopedilum barbatum (Lindl.) pfitzer from Ex situ location and determination of its growth-enhancing capabilities 2010-12 Idris, Nurul Aliaa Orchids and mycorrhiza are known to have a symbiotic relationship that function particularly in seed germination and also in promoting overall plant growth. It is believed that a certain orchid mycorrhiza that functions to enhance plant growth also lives symbiotically in the roots of Paphiopedilum barbatum. Thus, the objectives of this research are to identify the mycorrhizal fungi that form a symbiosis with the terrestrial tropical orchid, P. barbatum and evaluate its capabilities to enhance growth in certain in vitro Paphiopedilum seedlings. Mycorrhizal fungi were isolated from the roots of P. barbatum collected from five different locations in Peninsular Malaysia. Isolation of fungi was carried out according to the Currah method for initial morphological identification and then through molecular identification using the internal transcribed spacer (ITS) region of nuclear ribosomal DNA of the isolates. The identified mycorrhiza was then inoculated on in vitro selected Paphiopedilum seedlings on a double slanting media to observe mycorrhization and the occurrence of enhancement of plant growth. Only one mycorrhiza was isolated and identified; namely Tulasnella calospora (anamorph: Rhizoctonia repens) which is a ubiquitous orchid mycorrhiza. Phylogenetic analyses with sequences originating from different hosts from different countries indicated a close genetic relatedness of the sequences studied. In vitro inoculation of T. calospora on seedlings from the Paphiopedilum genus after incubation for 24 weeks showed a significant 1.1% increase in fresh weight in P. rothschildianum with 68% root mycorrhization. However, no significant growth was observed in the seedlings of P. sanderianum, P. gigantifolium x P. rothschildianum and P. esquirolei x P. rothschildianum when inoculated with T. calospora after incubation for 24 weeks even though each had 32%, 24% and 13% root mycorrhization. This preliminary finding present an insight into the orchid mycorrhiza of the Paphiopedilum but a more extensive study is needed to further explore this symbiotic relationship and furthermore contribute in developing a beneficial application whether commercially or for the conservation of this genus. Mycorrhizas Crops - Growth Paphiopedilum 2010-12 Thesis http://psasir.upm.edu.my/id/eprint/85130/ http://psasir.upm.edu.my/id/eprint/85130/1/IB%202010%205%20ir.pdf text en public masters Universiti Putra Malaysia Mycorrhizas Crops - Growth Paphiopedilum Qamaruz Zaman, Faridah
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Qamaruz Zaman, Faridah
topic	Mycorrhizas Crops - Growth Paphiopedilum
spellingShingle	Mycorrhizas Crops - Growth Paphiopedilum Idris, Nurul Aliaa Identification of a mycorrhiza isolate from Paphiopedilum barbatum (Lindl.) pfitzer from Ex situ location and determination of its growth-enhancing capabilities
description	Orchids and mycorrhiza are known to have a symbiotic relationship that function particularly in seed germination and also in promoting overall plant growth. It is believed that a certain orchid mycorrhiza that functions to enhance plant growth also lives symbiotically in the roots of Paphiopedilum barbatum. Thus, the objectives of this research are to identify the mycorrhizal fungi that form a symbiosis with the terrestrial tropical orchid, P. barbatum and evaluate its capabilities to enhance growth in certain in vitro Paphiopedilum seedlings. Mycorrhizal fungi were isolated from the roots of P. barbatum collected from five different locations in Peninsular Malaysia. Isolation of fungi was carried out according to the Currah method for initial morphological identification and then through molecular identification using the internal transcribed spacer (ITS) region of nuclear ribosomal DNA of the isolates. The identified mycorrhiza was then inoculated on in vitro selected Paphiopedilum seedlings on a double slanting media to observe mycorrhization and the occurrence of enhancement of plant growth. Only one mycorrhiza was isolated and identified; namely Tulasnella calospora (anamorph: Rhizoctonia repens) which is a ubiquitous orchid mycorrhiza. Phylogenetic analyses with sequences originating from different hosts from different countries indicated a close genetic relatedness of the sequences studied. In vitro inoculation of T. calospora on seedlings from the Paphiopedilum genus after incubation for 24 weeks showed a significant 1.1% increase in fresh weight in P. rothschildianum with 68% root mycorrhization. However, no significant growth was observed in the seedlings of P. sanderianum, P. gigantifolium x P. rothschildianum and P. esquirolei x P. rothschildianum when inoculated with T. calospora after incubation for 24 weeks even though each had 32%, 24% and 13% root mycorrhization. This preliminary finding present an insight into the orchid mycorrhiza of the Paphiopedilum but a more extensive study is needed to further explore this symbiotic relationship and furthermore contribute in developing a beneficial application whether commercially or for the conservation of this genus.
format	Thesis
qualification_level	Master's degree
author	Idris, Nurul Aliaa
author_facet	Idris, Nurul Aliaa
author_sort	Idris, Nurul Aliaa
title	Identification of a mycorrhiza isolate from Paphiopedilum barbatum (Lindl.) pfitzer from Ex situ location and determination of its growth-enhancing capabilities
title_short	Identification of a mycorrhiza isolate from Paphiopedilum barbatum (Lindl.) pfitzer from Ex situ location and determination of its growth-enhancing capabilities
title_full	Identification of a mycorrhiza isolate from Paphiopedilum barbatum (Lindl.) pfitzer from Ex situ location and determination of its growth-enhancing capabilities
title_fullStr	Identification of a mycorrhiza isolate from Paphiopedilum barbatum (Lindl.) pfitzer from Ex situ location and determination of its growth-enhancing capabilities
title_full_unstemmed	Identification of a mycorrhiza isolate from Paphiopedilum barbatum (Lindl.) pfitzer from Ex situ location and determination of its growth-enhancing capabilities
title_sort	identification of a mycorrhiza isolate from paphiopedilum barbatum (lindl.) pfitzer from ex situ location and determination of its growth-enhancing capabilities
granting_institution	Universiti Putra Malaysia
publishDate	2010
url	http://psasir.upm.edu.my/id/eprint/85130/1/IB%202010%205%20ir.pdf
_version_	1747813519673262080

Identification of a mycorrhiza isolate from Paphiopedilum barbatum (Lindl.) pfitzer from Ex situ location and determination of its growth-enhancing capabilities

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