Seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of GST genes among the coastal community of Melaka, Malaysia

Heavy metals such as arsenic (As) and lead (Pb) exposure through the consumption of seafood are an environmental issue of concern. Biomonitoring of whole blood heavy metals level gave an insight of the total internal body burden due to the exposure of heavy metals. The level of heavy metals of an in...

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Main Author:	Mohd Noor, Farrah Atiqah
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Arsenic -Toxicology Public health
Online Access:	http://psasir.upm.edu.my/id/eprint/85548/1/FPSK%28M%29%202019%2037%20UPM%20ir.pdf
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spelling	my-upm-ir.855482021-12-14T02:11:14Z Seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of GST genes among the coastal community of Melaka, Malaysia 2018-04 Mohd Noor, Farrah Atiqah Heavy metals such as arsenic (As) and lead (Pb) exposure through the consumption of seafood are an environmental issue of concern. Biomonitoring of whole blood heavy metals level gave an insight of the total internal body burden due to the exposure of heavy metals. The level of heavy metals of an individual are interrelated with the frequency of seafood intake and genetic variants of glutathione S-tranferases (GST). The objectives of this study were to determine the association between blood As and Pb concentration and socio-demographic background, potential sources of exposure and polymorphic variants of GST genes. This was a cross-sectional study conducted from September 2014 until February 2016 among 403 subjects with age ranging between 18 to 60 years old. Further blood analysis were voluntarily participated by 63 respondents. The socio-demographic information, seafood frequency intake and other potential exposures to heavy metals were obtained through pre-tested questionnaire. Heavy metals concentration in blood were determined by inductively coupled plasma- mass spectrometry (ICP-MS), polymorphic variants of GSTM1 and GSTT1 were analyzed by multiplex polymerase chain reaction (PCR) while polymorphic variants of GSTP1 were analyzed by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Majority of the respondents in Melaka were a frequent eater of fish (≥ 3 times per week) with percentage of 92.1% (n=403). The median blood arsenic (BAs) concentration of respondents was 0.076 μg/L (interquartile range: 0.048 μg/L) which range between 0.01 to 0.36 μg/L and the median blood lead (BPb) concentration of respondents was 1.204 μg/L (interquartile range: 1.424 μg/L) which range between 0.06 to 5.06 μg/L respectively (n=63). Expected prevalence of genetic polymorphism in GSTM1 and GSTT1 of the respondents of 63.5% and 31.8% respectively (n=63) were observed. The number of respondents who have both deleted genes for GSTM1 and GSTT1 was 15 (23.8%). The frequency of genetic polymorphism in GSTP1 was 55.6%, in which 29 (46.0%) of the respondents were having the heterozygous (Ile/Val) polymorphism while the other 6 (9.6%) respondents were having the homozygous mutant (Val/Val). A significant association was observed between seafood-based product frequency intake and BPb concentration (p < 0.05). Age, duration of settlement, household income, gender, educational level and BMI did not significantly associated with the BAs and BPb concentration of respondents. There were no significant relationship between seafood frequency intake, smoking and occupational exposure of study subjects with BAs and BPb concentration. The polymorphic GST variants were also found not significantly correlated to heavy metals concentrations. Arsenic -Toxicology Public health 2018-04 Thesis http://psasir.upm.edu.my/id/eprint/85548/ http://psasir.upm.edu.my/id/eprint/85548/1/FPSK%28M%29%202019%2037%20UPM%20ir.pdf text en public masters Universiti Putra Malaysia Arsenic -Toxicology Public health Mohd Elias, Saliza
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Mohd Elias, Saliza
topic	Arsenic -Toxicology Public health
spellingShingle	Arsenic -Toxicology Public health Mohd Noor, Farrah Atiqah Seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of GST genes among the coastal community of Melaka, Malaysia
description	Heavy metals such as arsenic (As) and lead (Pb) exposure through the consumption of seafood are an environmental issue of concern. Biomonitoring of whole blood heavy metals level gave an insight of the total internal body burden due to the exposure of heavy metals. The level of heavy metals of an individual are interrelated with the frequency of seafood intake and genetic variants of glutathione S-tranferases (GST). The objectives of this study were to determine the association between blood As and Pb concentration and socio-demographic background, potential sources of exposure and polymorphic variants of GST genes. This was a cross-sectional study conducted from September 2014 until February 2016 among 403 subjects with age ranging between 18 to 60 years old. Further blood analysis were voluntarily participated by 63 respondents. The socio-demographic information, seafood frequency intake and other potential exposures to heavy metals were obtained through pre-tested questionnaire. Heavy metals concentration in blood were determined by inductively coupled plasma- mass spectrometry (ICP-MS), polymorphic variants of GSTM1 and GSTT1 were analyzed by multiplex polymerase chain reaction (PCR) while polymorphic variants of GSTP1 were analyzed by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Majority of the respondents in Melaka were a frequent eater of fish (≥ 3 times per week) with percentage of 92.1% (n=403). The median blood arsenic (BAs) concentration of respondents was 0.076 μg/L (interquartile range: 0.048 μg/L) which range between 0.01 to 0.36 μg/L and the median blood lead (BPb) concentration of respondents was 1.204 μg/L (interquartile range: 1.424 μg/L) which range between 0.06 to 5.06 μg/L respectively (n=63). Expected prevalence of genetic polymorphism in GSTM1 and GSTT1 of the respondents of 63.5% and 31.8% respectively (n=63) were observed. The number of respondents who have both deleted genes for GSTM1 and GSTT1 was 15 (23.8%). The frequency of genetic polymorphism in GSTP1 was 55.6%, in which 29 (46.0%) of the respondents were having the heterozygous (Ile/Val) polymorphism while the other 6 (9.6%) respondents were having the homozygous mutant (Val/Val). A significant association was observed between seafood-based product frequency intake and BPb concentration (p < 0.05). Age, duration of settlement, household income, gender, educational level and BMI did not significantly associated with the BAs and BPb concentration of respondents. There were no significant relationship between seafood frequency intake, smoking and occupational exposure of study subjects with BAs and BPb concentration. The polymorphic GST variants were also found not significantly correlated to heavy metals concentrations.
format	Thesis
qualification_level	Master's degree
author	Mohd Noor, Farrah Atiqah
author_facet	Mohd Noor, Farrah Atiqah
author_sort	Mohd Noor, Farrah Atiqah
title	Seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of GST genes among the coastal community of Melaka, Malaysia
title_short	Seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of GST genes among the coastal community of Melaka, Malaysia
title_full	Seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of GST genes among the coastal community of Melaka, Malaysia
title_fullStr	Seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of GST genes among the coastal community of Melaka, Malaysia
title_full_unstemmed	Seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of GST genes among the coastal community of Melaka, Malaysia
title_sort	seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of gst genes among the coastal community of melaka, malaysia
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/85548/1/FPSK%28M%29%202019%2037%20UPM%20ir.pdf
_version_	1747813558053240832

Seafood consumption pattern and its relationship with blood arsenic, blood lead and genetic polymorphism of GST genes among the coastal community of Melaka, Malaysia

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