Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe

Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe

Ac DNA library was con structed inphagemid vector pT7T3 using messenger RNA (mRNA) extracted from IS - weekoil palm me socarp as template. The cDNA inserts were detected from agarose gel analysis and ranged in length from 130 to 1300 base pairs.This library was screened with a synthetic ACP pro...

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Bibliographic Details
Main Author: Abd. Manaf, Mohamad Arif
Format: Thesis
Language:English
Published: 1994
Subjects:
Carrier proteins
DNA-protein interactions
Oil palm
Online Access:http://psasir.upm.edu.my/id/eprint/8588/1/FSAS_1994_6_A.pdf
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spelling my-upm-ir.85882015-05-14T07:22:07Z Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe 1994 Abd. Manaf, Mohamad Arif Ac DNA library was con structed inphagemid vector pT7T3 using messenger RNA (mRNA) extracted from IS - weekoil palm me socarp as template. The cDNA inserts were detected from agarose gel analysis and ranged in length from 130 to 1300 base pairs.This library was screened with a synthetic ACP probe.Of the 2,000 clones screened using colony hybridisation analysis, 550 clones which gave high intensity of signal were then further screened using phagemid blot hybridisation analysis. This met hodyielded 184 clones which showed a high intensity of signal. The phagemids from the seclones were then screened by Southern hybridisation analysis using a synthetic ACP DNA probe. Carrier proteins DNA-protein interactions Oil palm 1994 Thesis http://psasir.upm.edu.my/id/eprint/8588/ http://psasir.upm.edu.my/id/eprint/8588/1/FSAS_1994_6_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Carrier proteins DNA-protein interactions Oil palm Faculty of Environmental Studies
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
topic Carrier proteins
DNA-protein interactions
Oil palm
spellingShingle Carrier proteins
DNA-protein interactions
Oil palm
Abd. Manaf, Mohamad Arif
Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
description Ac DNA library was con structed inphagemid vector pT7T3 using messenger RNA (mRNA) extracted from IS - weekoil palm me socarp as template. The cDNA inserts were detected from agarose gel analysis and ranged in length from 130 to 1300 base pairs.This library was screened with a synthetic ACP probe.Of the 2,000 clones screened using colony hybridisation analysis, 550 clones which gave high intensity of signal were then further screened using phagemid blot hybridisation analysis. This met hodyielded 184 clones which showed a high intensity of signal. The phagemids from the seclones were then screened by Southern hybridisation analysis using a synthetic ACP DNA probe.
format Thesis
qualification_level Master's degree
author Abd. Manaf, Mohamad Arif
author_facet Abd. Manaf, Mohamad Arif
author_sort Abd. Manaf, Mohamad Arif
title Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_short Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_full Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_fullStr Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_full_unstemmed Screening for Acyl Carrier Protein (ACP) Gene of Oil Palm (Elaeis Quineensia) Using a Synthetic ACP DNA Probe
title_sort screening for acyl carrier protein (acp) gene of oil palm (elaeis quineensia) using a synthetic acp dna probe
granting_institution Universiti Pertanian Malaysia
granting_department Faculty of Environmental Studies
publishDate 1994
url http://psasir.upm.edu.my/id/eprint/8588/1/FSAS_1994_6_A.pdf
_version_ 1747810833067409408

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