Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM
Newcastle disease virus (NOV) is the prototy peavian Paramyxovirus which causes a major disease in poultry. The highlyvi rulentv elogenic NDV strain AF 2240 andavi rulent substrainV4 - UPM possess thermost a blehaemag glutination andneura minidase activities compared to other strains. Therefore,...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1994
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/8590/1/FSAS_1994_9_A.pdf |
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| Summary: | Newcastle disease virus (NOV) is the prototy peavian Paramyxovirus which causes a major disease in
poultry. The highlyvi rulentv elogenic NDV strain
AF 2240 andavi rulent substrainV4 - UPM possess
thermost a blehaemag glutination andneura minidase
activities compared to other strains. Therefore,the
purpose of this study is to sequence the
ha emagglutinin- neura minidase(HN)genes of AF 2240 and
V4-UPM.
The virus was propagated in the allantoic fluid of
9-10day-old embryonated eggs. The allantoic fluid was
harvested after 72 hours incubation at 37°C. Viral
purification was carried out on 30% to 60% (N/V )
sucrose density gradient centrifugation at 2 75,000 x g,
for 4 hour sat 4°C. The viral RNA was extracted using the hot phenol extraction method. RNA sequencing was
performed directly on the genomic RNA using the dideoxy
chain termination method. The nucleotide sequence was
then confirmed by cycle sequencing and analysed using
the computer programs of RIBIODNASIS and MicroGenie
which were linked to the Data Bank . |
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