Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM

Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM

Newcastle disease virus (NOV) is the prototy peavian Paramyxovirus which causes a major disease in poultry. The highlyvi rulentv elogenic NDV strain AF 2240 andavi rulent substrainV4 - UPM possess thermost a blehaemag glutination andneura minidase activities compared to other strains. Therefore,...

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Main Author: Tan, Wen Siang
Format: Thesis
Language:English
English
Published: 1994
Subjects:
Nucleotide sequence - Case studies
Newcastle disease virus - Case studies
Online Access:http://psasir.upm.edu.my/id/eprint/8590/1/FSAS_1994_9_A.pdf
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spelling my-upm-ir.85902012-05-07T08:56:36Z Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM 1994 Tan, Wen Siang Newcastle disease virus (NOV) is the prototy peavian Paramyxovirus which causes a major disease in poultry. The highlyvi rulentv elogenic NDV strain AF 2240 andavi rulent substrainV4 - UPM possess thermost a blehaemag glutination andneura minidase activities compared to other strains. Therefore,the purpose of this study is to sequence the ha emagglutinin- neura minidase(HN)genes of AF 2240 and V4-UPM. The virus was propagated in the allantoic fluid of 9-10day-old embryonated eggs. The allantoic fluid was harvested after 72 hours incubation at 37°C. Viral purification was carried out on 30% to 60% (N/V ) sucrose density gradient centrifugation at 2 75,000 x g, for 4 hour sat 4°C. The viral RNA was extracted using the hot phenol extraction method. RNA sequencing was performed directly on the genomic RNA using the dideoxy chain termination method. The nucleotide sequence was then confirmed by cycle sequencing and analysed using the computer programs of RIBIODNASIS and MicroGenie which were linked to the Data Bank . Nucleotide sequence - Case studies Newcastle disease virus - Case studies 1994 Thesis http://psasir.upm.edu.my/id/eprint/8590/ http://psasir.upm.edu.my/id/eprint/8590/1/FSAS_1994_9_A.pdf application/pdf en public masters Universiti Pertanian Malaysia Nucleotide sequence - Case studies Newcastle disease virus - Case studies Faculty of Environmental Studies English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Nucleotide sequence - Case studies
Newcastle disease virus - Case studies
spellingShingle Nucleotide sequence - Case studies
Newcastle disease virus - Case studies

Tan, Wen Siang
Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM
description Newcastle disease virus (NOV) is the prototy peavian Paramyxovirus which causes a major disease in poultry. The highlyvi rulentv elogenic NDV strain AF 2240 andavi rulent substrainV4 - UPM possess thermost a blehaemag glutination andneura minidase activities compared to other strains. Therefore,the purpose of this study is to sequence the ha emagglutinin- neura minidase(HN)genes of AF 2240 and V4-UPM. The virus was propagated in the allantoic fluid of 9-10day-old embryonated eggs. The allantoic fluid was harvested after 72 hours incubation at 37°C. Viral purification was carried out on 30% to 60% (N/V ) sucrose density gradient centrifugation at 2 75,000 x g, for 4 hour sat 4°C. The viral RNA was extracted using the hot phenol extraction method. RNA sequencing was performed directly on the genomic RNA using the dideoxy chain termination method. The nucleotide sequence was then confirmed by cycle sequencing and analysed using the computer programs of RIBIODNASIS and MicroGenie which were linked to the Data Bank .
format Thesis
qualification_level Master's degree
author Tan, Wen Siang
author_facet Tan, Wen Siang
author_sort Tan, Wen Siang
title Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM
title_short Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM
title_full Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM
title_fullStr Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM
title_full_unstemmed Nucleotide Sequence Analysis of the HN Gene of the Heat Resistant NDV Strain Af2240 and Substrain V4-UPM
title_sort nucleotide sequence analysis of the hn gene of the heat resistant ndv strain af2240 and substrain v4-upm
granting_institution Universiti Pertanian Malaysia
granting_department Faculty of Environmental Studies
publishDate 1994
url http://psasir.upm.edu.my/id/eprint/8590/1/FSAS_1994_9_A.pdf
_version_ 1747810833549754368

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