The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
Herbicides have been found to be significant pollutants of water bodies. In order to control and monitor the pollution, a convenient, sensitive and reliable method is needed. The main objective of this study is to develop a new method to measure the fluorescence yield of microalgae in order to d...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1997
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/8634/1/FSAS_1997_19_A.pdf |
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| Summary: | Herbicides have been found to be significant pollutants of water
bodies. In order to control and monitor the pollution, a convenient,
sensitive and reliable method is needed. The main objective of this
study is to develop a new method to measure the fluorescence yield of
microalgae in order to detect photosystem II (PSII) specific herbicides
in polluted water. The system was based on a simple mixing of
microalgae with PSII specific herbicides. The measured signal was in the form of fluorescence intensity. The study involved the screening of
test organisms, determining the best condition of the culture and
obtaining a standard curve for each of the PSII herbicides. Only three
herbicides were studied; atrazine, simazine and diuron. Chlorella
vulgaris was found as the most suitable test organism compared with
Chlorococcum sp., Scenedesmus quadriqauda and Selenastrum sp ..
Nutrient replacement culturing method was selected as the best
condition to grow C. vulgaris. A standard curve was determined for
each of the herbicides. C. vulgaris was able to signal atrazine's
concentration above 0.04µM according to the standard curve equation.
Simazine can be signalled at above 0.05µM from its equation, while
diuron can be signalled at above than O.09µM. However, -this basic
study needs more infonnation and improvement to be done in the
future, so that its full potential can be realised. |
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