The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor

Herbicides have been found to be significant pollutants of water bodies. In order to control and monitor the pollution, a convenient, sensitive and reliable method is needed. The main objective of this study is to develop a new method to measure the fluorescence yield of microalgae in order to d...

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Main Author:	Desa, Shakinaz
Format:	Thesis
Language:	English English
Published:	1997
Subjects:	Microalgae Herbicides - Physiological effect
Online Access:	http://psasir.upm.edu.my/id/eprint/8634/1/FSAS_1997_19_A.pdf
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id	my-upm-ir.8634
record_format	uketd_dc
spelling	my-upm-ir.86342012-05-08T09:20:00Z The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor 1997 Desa, Shakinaz Herbicides have been found to be significant pollutants of water bodies. In order to control and monitor the pollution, a convenient, sensitive and reliable method is needed. The main objective of this study is to develop a new method to measure the fluorescence yield of microalgae in order to detect photosystem II (PSII) specific herbicides in polluted water. The system was based on a simple mixing of microalgae with PSII specific herbicides. The measured signal was in the form of fluorescence intensity. The study involved the screening of test organisms, determining the best condition of the culture and obtaining a standard curve for each of the PSII herbicides. Only three herbicides were studied; atrazine, simazine and diuron. Chlorella vulgaris was found as the most suitable test organism compared with Chlorococcum sp., Scenedesmus quadriqauda and Selenastrum sp .. Nutrient replacement culturing method was selected as the best condition to grow C. vulgaris. A standard curve was determined for each of the herbicides. C. vulgaris was able to signal atrazine's concentration above 0.04µM according to the standard curve equation. Simazine can be signalled at above 0.05µM from its equation, while diuron can be signalled at above than O.09µM. However, -this basic study needs more infonnation and improvement to be done in the future, so that its full potential can be realised. Microalgae Herbicides - Physiological effect 1997 Thesis http://psasir.upm.edu.my/id/eprint/8634/ http://psasir.upm.edu.my/id/eprint/8634/1/FSAS_1997_19_A.pdf application/pdf en public masters Universiti Putra Malaysia Microalgae Herbicides - Physiological effect Faculty of Environmental Studies English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Microalgae Herbicides - Physiological effect
spellingShingle	Microalgae Herbicides - Physiological effect Desa, Shakinaz The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
description	Herbicides have been found to be significant pollutants of water bodies. In order to control and monitor the pollution, a convenient, sensitive and reliable method is needed. The main objective of this study is to develop a new method to measure the fluorescence yield of microalgae in order to detect photosystem II (PSII) specific herbicides in polluted water. The system was based on a simple mixing of microalgae with PSII specific herbicides. The measured signal was in the form of fluorescence intensity. The study involved the screening of test organisms, determining the best condition of the culture and obtaining a standard curve for each of the PSII herbicides. Only three herbicides were studied; atrazine, simazine and diuron. Chlorella vulgaris was found as the most suitable test organism compared with Chlorococcum sp., Scenedesmus quadriqauda and Selenastrum sp .. Nutrient replacement culturing method was selected as the best condition to grow C. vulgaris. A standard curve was determined for each of the herbicides. C. vulgaris was able to signal atrazine's concentration above 0.04µM according to the standard curve equation. Simazine can be signalled at above 0.05µM from its equation, while diuron can be signalled at above than O.09µM. However, -this basic study needs more infonnation and improvement to be done in the future, so that its full potential can be realised.
format	Thesis
qualification_level	Master's degree
author	Desa, Shakinaz
author_facet	Desa, Shakinaz
author_sort	Desa, Shakinaz
title	The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
title_short	The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
title_full	The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
title_fullStr	The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
title_full_unstemmed	The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor
title_sort	development of photosystem ii-herbicide detection method using microalgae as a biosensor
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Environmental Studies
publishDate	1997
url	http://psasir.upm.edu.my/id/eprint/8634/1/FSAS_1997_19_A.pdf
_version_	1747810844333309952

The Development of Photosystem II-Herbicide Detection Method Using Microalgae as a Biosensor

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