Immobilization of Candida Rugosa lipase onto N-Vinyl-2-Pyyrolidone-Co-Styrene Hydrogel for use in enantioselective esterification

Lipase from Candida rugosa was immobilized onto N-vinyl-2- pyyrolidone-co-styrene hydrogel (VP-co-ST) The crosslinker and the initiator used were EDMA and AIBN respectively Three different compositions of monomer were used, namely, (VP.ST)% , 1090, 5050 and 70 30 The VP-co ST hydrogel immobilize...

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Main Author:	Harun, Aiza
Format:	Thesis
Language:	English English
Published:	1999
Subjects:	Esterification Immobilized enzymes
Online Access:	http://psasir.upm.edu.my/id/eprint/8660/1/FSAS_1999_23_IR.pdf
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id	my-upm-ir.8660
record_format	uketd_dc
spelling	my-upm-ir.86602023-12-14T00:27:08Z Immobilization of Candida Rugosa lipase onto N-Vinyl-2-Pyyrolidone-Co-Styrene Hydrogel for use in enantioselective esterification 1999-12 Harun, Aiza Lipase from Candida rugosa was immobilized onto N-vinyl-2- pyyrolidone-co-styrene hydrogel (VP-co-ST) The crosslinker and the initiator used were EDMA and AIBN respectively Three different compositions of monomer were used, namely, (VP.ST)% , 1090, 5050 and 70 30 The VP-co ST hydrogel immobilized lipases were used in the enantioselective esterification of (R,S)-2-(4-chlorophenoxy) propanoic acid with n-tetradecanol The optimum reaction time was 24h for native and all immobilized lipases StudIes on the effect of percentages composition of monomers of the VP-co-ST hydrogel immobilized lipases showed that lipase immobilized onto hydrogel (VP ST)%, 1090 exhibited the highest percentages of enantiomeric excess at approximately 24% Generally, increasing the temperature of the enantioselective esterification increases the percentage of ester conversion for all VP-co-ST hydrogel immobilized lipases However, the percentages of enantiomeric excess increases up to 40°C and subsequently decreases At 40°C, the highest percentage of enantiomeric excess was shown by VP-co-ST hydrogel with compositlOn of monomer (VP ST)%, 1090 at 45% In the solvent effect studies, the percentage of ester conversion was higher in relative more polar organic solvents with log P value ranging from 20 to 30 for all immobilized lipases and native lipase The organic solvents such as toluene, chloroform and carbon tetrachloride showed higher percentage of ester conversion as compared with other solvents Immobilized lipases exhibited higher percentage of enantiomeric excess compared to the native lipase Higher percentage of enantiomeric excess were obtained when using chloroform and carbon tetrachloride at approximately 50% and 46% for VP-co-ST (VP ST)%, 5050 and (VP ST)%, 1090 respectively In the water activity studies, at optimum a, all immobilized lipases showed higher percentage of ester conversion compared to the native lipase at approximately 23%, 16% and 22% for VP-co-ST hydrogel with composition of monomer (VP ST)%, 1090, 5050 and 7030 respectively At optimum all, VPco- ST hydrogel immobilized lipase with composition of monomer (VP ST)%, 1090, 5050 and 7030 exhibited higher percentage of enantiomeric excess at approximately 40%, 21% and 28% respectively compared to the native lipase. Esterification Immobilized enzymes 1999-12 Thesis http://psasir.upm.edu.my/id/eprint/8660/ http://psasir.upm.edu.my/id/eprint/8660/1/FSAS_1999_23_IR.pdf text en public masters Universiti Putra Malaysia Esterification Immobilized enzymes Faculty of Environmental Studies Basri, Mahiran English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Basri, Mahiran
topic	Esterification Immobilized enzymes
spellingShingle	Esterification Immobilized enzymes Harun, Aiza Immobilization of Candida Rugosa lipase onto N-Vinyl-2-Pyyrolidone-Co-Styrene Hydrogel for use in enantioselective esterification
description	Lipase from Candida rugosa was immobilized onto N-vinyl-2- pyyrolidone-co-styrene hydrogel (VP-co-ST) The crosslinker and the initiator used were EDMA and AIBN respectively Three different compositions of monomer were used, namely, (VP.ST)% , 1090, 5050 and 70 30 The VP-co ST hydrogel immobilized lipases were used in the enantioselective esterification of (R,S)-2-(4-chlorophenoxy) propanoic acid with n-tetradecanol The optimum reaction time was 24h for native and all immobilized lipases StudIes on the effect of percentages composition of monomers of the VP-co-ST hydrogel immobilized lipases showed that lipase immobilized onto hydrogel (VP ST)%, 1090 exhibited the highest percentages of enantiomeric excess at approximately 24% Generally, increasing the temperature of the enantioselective esterification increases the percentage of ester conversion for all VP-co-ST hydrogel immobilized lipases However, the percentages of enantiomeric excess increases up to 40°C and subsequently decreases At 40°C, the highest percentage of enantiomeric excess was shown by VP-co-ST hydrogel with compositlOn of monomer (VP ST)%, 1090 at 45% In the solvent effect studies, the percentage of ester conversion was higher in relative more polar organic solvents with log P value ranging from 20 to 30 for all immobilized lipases and native lipase The organic solvents such as toluene, chloroform and carbon tetrachloride showed higher percentage of ester conversion as compared with other solvents Immobilized lipases exhibited higher percentage of enantiomeric excess compared to the native lipase Higher percentage of enantiomeric excess were obtained when using chloroform and carbon tetrachloride at approximately 50% and 46% for VP-co-ST (VP ST)%, 5050 and (VP ST)%, 1090 respectively In the water activity studies, at optimum a, all immobilized lipases showed higher percentage of ester conversion compared to the native lipase at approximately 23%, 16% and 22% for VP-co-ST hydrogel with composition of monomer (VP ST)%, 1090, 5050 and 7030 respectively At optimum all, VPco- ST hydrogel immobilized lipase with composition of monomer (VP ST)%, 1090, 5050 and 7030 exhibited higher percentage of enantiomeric excess at approximately 40%, 21% and 28% respectively compared to the native lipase.
format	Thesis
qualification_level	Master's degree
author	Harun, Aiza
author_facet	Harun, Aiza
author_sort	Harun, Aiza
title	Immobilization of Candida Rugosa lipase onto N-Vinyl-2-Pyyrolidone-Co-Styrene Hydrogel for use in enantioselective esterification
title_short	Immobilization of Candida Rugosa lipase onto N-Vinyl-2-Pyyrolidone-Co-Styrene Hydrogel for use in enantioselective esterification
title_full	Immobilization of Candida Rugosa lipase onto N-Vinyl-2-Pyyrolidone-Co-Styrene Hydrogel for use in enantioselective esterification
title_fullStr	Immobilization of Candida Rugosa lipase onto N-Vinyl-2-Pyyrolidone-Co-Styrene Hydrogel for use in enantioselective esterification
title_full_unstemmed	Immobilization of Candida Rugosa lipase onto N-Vinyl-2-Pyyrolidone-Co-Styrene Hydrogel for use in enantioselective esterification
title_sort	immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene hydrogel for use in enantioselective esterification
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Environmental Studies
publishDate	1999
url	http://psasir.upm.edu.my/id/eprint/8660/1/FSAS_1999_23_IR.pdf
_version_	1794018768433709056

Immobilization of Candida Rugosa lipase onto N-Vinyl-2-Pyyrolidone-Co-Styrene Hydrogel for use in enantioselective esterification

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