Symbiotic relationship between selected probiotic bacteria in chemostat culture
The effect of culture combination on growth of three strains of probiotic (Streptococcus jaecalis T-110, Bacillus mesentericus TO-A and Bifidobacterium breve G48) was examined using chemostat culture. Results of bacterial viable counts revealed that mixed culture of two probiotic microorganisms h...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2000
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/8720/1/FSMB_2000_8%20IR.pdf |
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| Summary: | The effect of culture combination on growth of three strains of probiotic
(Streptococcus jaecalis T-110, Bacillus mesentericus TO-A and Bifidobacterium
breve G48) was examined using chemostat culture. Results of bacterial viable counts
revealed that mixed culture of two probiotic microorganisms had higher growth rate
compared to single culture of probiotic. The improvement of bacterial growth can be
partly attributed to the production of certain growth factors by one microorganism
that was capable of stimulating the growth of another bacterium. The metabolites in
sample broth were determined using High Performance Liquid Chromatography (HPLC). It can be postulated that succmlC acid, argmme, vitamin B₁ and B₂
synthesised by B. breve 048 were used by S. faecalis T -110 to improve its growth.
Furthennore, B. mesentericus TO-A was found to utilise vitamin B6 and fonnic acid
produced by the respective S. faecalis T -110 and B. breve TO-A. In return, B. breve
048 utilised vitamin BI2 produced by B. mesentericus TO-A. The effect of mixed
culture of probiotic on growth of pathogenic Escherichia coli V517 was also
investigated in chemostat culture. Results showed that mixed culture of probiotic
exerted better inhibitory effect against E. coli V 517 compared to single culture of
probiotic. HPLC analyses showed that mixed culture of probiotic produced higher
yield of lactic and acetic acids that are fatal to E. coli V 517. Meanwhile, ammonia
was found not to be an important inhibitory agent to E. coli V 517. Studies on the
effects of metabolites on growth of probiotic organisms as well as E. coli V 517 were
also carried out to validate the hypotheses made in previous experiments. The results
revealed that growth of tested probiotic bacteria increased with certain level of
substrate concentration. In contrast, growth of E. coli V517 decreased with increasing
concentration of lactic and acetic acids. It was postulated that mixed culture of S.
faecalis T -110 and B. breve 048 as well as co-culture of S. faecalis T -110 and B.
mesentericus TO-A exhibited commensal relationship, in which only one party
benefits an the other is neither hanned nor benefited. Meanwhile, mixed culture of B.
mesentericus TO-A and B. breve 048 showed a mutualistic association whereby both
organisms profit from each other. Finally, in mixed culture of E. coli V517 and
probiotic organisms, a strong antagonistic relationship was observed. |
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