Symbiotic relationship between selected probiotic bacteria in chemostat culture

The effect of culture combination on growth of three strains of probiotic (Streptococcus jaecalis T-110, Bacillus mesentericus TO-A and Bifidobacterium breve G48) was examined using chemostat culture. Results of bacterial viable counts revealed that mixed culture of two probiotic microorganisms h...

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Main Author:	Jusoh, Normah
Format:	Thesis
Language:	English English
Published:	2000
Subjects:	Bacteria - Health aspects Probiotics Biological Factors
Online Access:	http://psasir.upm.edu.my/id/eprint/8720/1/FSMB_2000_8%20IR.pdf
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id	my-upm-ir.8720
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spelling	my-upm-ir.87202023-12-28T03:26:28Z Symbiotic relationship between selected probiotic bacteria in chemostat culture 2000-04 Jusoh, Normah The effect of culture combination on growth of three strains of probiotic (Streptococcus jaecalis T-110, Bacillus mesentericus TO-A and Bifidobacterium breve G48) was examined using chemostat culture. Results of bacterial viable counts revealed that mixed culture of two probiotic microorganisms had higher growth rate compared to single culture of probiotic. The improvement of bacterial growth can be partly attributed to the production of certain growth factors by one microorganism that was capable of stimulating the growth of another bacterium. The metabolites in sample broth were determined using High Performance Liquid Chromatography (HPLC). It can be postulated that succmlC acid, argmme, vitamin B₁ and B₂ synthesised by B. breve 048 were used by S. faecalis T -110 to improve its growth. Furthennore, B. mesentericus TO-A was found to utilise vitamin B6 and fonnic acid produced by the respective S. faecalis T -110 and B. breve TO-A. In return, B. breve 048 utilised vitamin BI2 produced by B. mesentericus TO-A. The effect of mixed culture of probiotic on growth of pathogenic Escherichia coli V517 was also investigated in chemostat culture. Results showed that mixed culture of probiotic exerted better inhibitory effect against E. coli V 517 compared to single culture of probiotic. HPLC analyses showed that mixed culture of probiotic produced higher yield of lactic and acetic acids that are fatal to E. coli V 517. Meanwhile, ammonia was found not to be an important inhibitory agent to E. coli V 517. Studies on the effects of metabolites on growth of probiotic organisms as well as E. coli V 517 were also carried out to validate the hypotheses made in previous experiments. The results revealed that growth of tested probiotic bacteria increased with certain level of substrate concentration. In contrast, growth of E. coli V517 decreased with increasing concentration of lactic and acetic acids. It was postulated that mixed culture of S. faecalis T -110 and B. breve 048 as well as co-culture of S. faecalis T -110 and B. mesentericus TO-A exhibited commensal relationship, in which only one party benefits an the other is neither hanned nor benefited. Meanwhile, mixed culture of B. mesentericus TO-A and B. breve 048 showed a mutualistic association whereby both organisms profit from each other. Finally, in mixed culture of E. coli V517 and probiotic organisms, a strong antagonistic relationship was observed. Bacteria - Health aspects Probiotics Biological Factors 2000-04 Thesis http://psasir.upm.edu.my/id/eprint/8720/ http://psasir.upm.edu.my/id/eprint/8720/1/FSMB_2000_8%20IR.pdf text en public masters Universiti Putra Malaysia Bacteria - Health aspects Probiotics Biological Factors Faculty of Food Science and Technology Abdul Manap, Mohd Yazid English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Abdul Manap, Mohd Yazid
topic	Bacteria - Health aspects Probiotics Biological Factors
spellingShingle	Bacteria - Health aspects Probiotics Biological Factors Jusoh, Normah Symbiotic relationship between selected probiotic bacteria in chemostat culture
description	The effect of culture combination on growth of three strains of probiotic (Streptococcus jaecalis T-110, Bacillus mesentericus TO-A and Bifidobacterium breve G48) was examined using chemostat culture. Results of bacterial viable counts revealed that mixed culture of two probiotic microorganisms had higher growth rate compared to single culture of probiotic. The improvement of bacterial growth can be partly attributed to the production of certain growth factors by one microorganism that was capable of stimulating the growth of another bacterium. The metabolites in sample broth were determined using High Performance Liquid Chromatography (HPLC). It can be postulated that succmlC acid, argmme, vitamin B₁ and B₂ synthesised by B. breve 048 were used by S. faecalis T -110 to improve its growth. Furthennore, B. mesentericus TO-A was found to utilise vitamin B6 and fonnic acid produced by the respective S. faecalis T -110 and B. breve TO-A. In return, B. breve 048 utilised vitamin BI2 produced by B. mesentericus TO-A. The effect of mixed culture of probiotic on growth of pathogenic Escherichia coli V517 was also investigated in chemostat culture. Results showed that mixed culture of probiotic exerted better inhibitory effect against E. coli V 517 compared to single culture of probiotic. HPLC analyses showed that mixed culture of probiotic produced higher yield of lactic and acetic acids that are fatal to E. coli V 517. Meanwhile, ammonia was found not to be an important inhibitory agent to E. coli V 517. Studies on the effects of metabolites on growth of probiotic organisms as well as E. coli V 517 were also carried out to validate the hypotheses made in previous experiments. The results revealed that growth of tested probiotic bacteria increased with certain level of substrate concentration. In contrast, growth of E. coli V517 decreased with increasing concentration of lactic and acetic acids. It was postulated that mixed culture of S. faecalis T -110 and B. breve 048 as well as co-culture of S. faecalis T -110 and B. mesentericus TO-A exhibited commensal relationship, in which only one party benefits an the other is neither hanned nor benefited. Meanwhile, mixed culture of B. mesentericus TO-A and B. breve 048 showed a mutualistic association whereby both organisms profit from each other. Finally, in mixed culture of E. coli V517 and probiotic organisms, a strong antagonistic relationship was observed.
format	Thesis
qualification_level	Master's degree
author	Jusoh, Normah
author_facet	Jusoh, Normah
author_sort	Jusoh, Normah
title	Symbiotic relationship between selected probiotic bacteria in chemostat culture
title_short	Symbiotic relationship between selected probiotic bacteria in chemostat culture
title_full	Symbiotic relationship between selected probiotic bacteria in chemostat culture
title_fullStr	Symbiotic relationship between selected probiotic bacteria in chemostat culture
title_full_unstemmed	Symbiotic relationship between selected probiotic bacteria in chemostat culture
title_sort	symbiotic relationship between selected probiotic bacteria in chemostat culture
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Technology
publishDate	2000
url	http://psasir.upm.edu.my/id/eprint/8720/1/FSMB_2000_8%20IR.pdf
_version_	1794018776370380800

Symbiotic relationship between selected probiotic bacteria in chemostat culture

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