Agrobacterium-mediated transformation of oil palm (Elaeis guineensis jacq.) suspension culture

The life cycle of flowering plants in general can be divided into two growth phases: vegetative and reproductive. The reproductive phase can be subdivided into the development of the inflorescence meristem and floral meristems. Control of flowering and the regulation of plant architecture has bee...

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Main Author:	Roowi, Siti Habsah
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Agrobacterium Angiosperms Oil palm
Online Access:	http://psasir.upm.edu.my/id/eprint/8726/1/FSMB_2003_19%20IR.pdf
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id	my-upm-ir.8726
record_format	uketd_dc
spelling	my-upm-ir.87262024-01-23T07:30:09Z Agrobacterium-mediated transformation of oil palm (Elaeis guineensis jacq.) suspension culture 2003-06 Roowi, Siti Habsah The life cycle of flowering plants in general can be divided into two growth phases: vegetative and reproductive. The reproductive phase can be subdivided into the development of the inflorescence meristem and floral meristems. Control of flowering and the regulation of plant architecture has been thoroughly investigated in a number of wellstudied dicot plants such as Arabidopsis, Antirrhinum, tomato and tobacco. However, in monocot plants, molecular information related to plant reproduction is still limited. In A rabidopsis , the Terminal Flowering I(TFLI) gene, LEAFY, and the target genes of CONSTANS (CO) including the FLOWERING LOCUS T (FI) and SUPPRESSOR OF OVEREXPRESSION OF COI (SOCI) genes, have a major role in promoting flowering and thus controlling flowering time. To investigate the regulation of meristem identity as well as the control of floral transition in oil palm, we transferred genes pCAMBIA/TFL1, pCAMBIA/JIT60, pCAMBIA/LFY, pGA/LFY and pCAMBIA/SOCl into oil palm embryogenic callus. This present study focuses on the optimization of Agrobacterium-mediated transformation and the analysis of transgenic plants. The objective of this study was also to clone the putative oil palm (OPSOCI and OPLFy) genes into a plasmid binary vector system, so as to transform these genes into oil palm embryogenic callus and to determine their effect on expression driven by a cauliflower mosaic virus (CaMV) 35S promoter in oil palm. The success of gene delivery using Agrobacterium into the plant genome is often based on several factors including temperature used during co-cultivation, the binary vector and promoters used, and the plant genotype itself. The most important factors contributing to the success of T -DNA transfer is the type of plant material used. We used embryogenic suspension cells as starting material for the transformation of oil palm because of the large numbers of totipotent cells found in these cultures. Agrobacterium Angiosperms Oil palm 2003-06 Thesis http://psasir.upm.edu.my/id/eprint/8726/ http://psasir.upm.edu.my/id/eprint/8726/1/FSMB_2003_19%20IR.pdf text en public masters Universiti Putra Malaysia Agrobacterium Angiosperms Oil palm Faculty of Food Science and Technology Harikrishna, K. English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Harikrishna, K.
topic	Agrobacterium Angiosperms Oil palm
spellingShingle	Agrobacterium Angiosperms Oil palm Roowi, Siti Habsah Agrobacterium-mediated transformation of oil palm (Elaeis guineensis jacq.) suspension culture
description	The life cycle of flowering plants in general can be divided into two growth phases: vegetative and reproductive. The reproductive phase can be subdivided into the development of the inflorescence meristem and floral meristems. Control of flowering and the regulation of plant architecture has been thoroughly investigated in a number of wellstudied dicot plants such as Arabidopsis, Antirrhinum, tomato and tobacco. However, in monocot plants, molecular information related to plant reproduction is still limited. In A rabidopsis , the Terminal Flowering I(TFLI) gene, LEAFY, and the target genes of CONSTANS (CO) including the FLOWERING LOCUS T (FI) and SUPPRESSOR OF OVEREXPRESSION OF COI (SOCI) genes, have a major role in promoting flowering and thus controlling flowering time. To investigate the regulation of meristem identity as well as the control of floral transition in oil palm, we transferred genes pCAMBIA/TFL1, pCAMBIA/JIT60, pCAMBIA/LFY, pGA/LFY and pCAMBIA/SOCl into oil palm embryogenic callus. This present study focuses on the optimization of Agrobacterium-mediated transformation and the analysis of transgenic plants. The objective of this study was also to clone the putative oil palm (OPSOCI and OPLFy) genes into a plasmid binary vector system, so as to transform these genes into oil palm embryogenic callus and to determine their effect on expression driven by a cauliflower mosaic virus (CaMV) 35S promoter in oil palm. The success of gene delivery using Agrobacterium into the plant genome is often based on several factors including temperature used during co-cultivation, the binary vector and promoters used, and the plant genotype itself. The most important factors contributing to the success of T -DNA transfer is the type of plant material used. We used embryogenic suspension cells as starting material for the transformation of oil palm because of the large numbers of totipotent cells found in these cultures.
format	Thesis
qualification_level	Master's degree
author	Roowi, Siti Habsah
author_facet	Roowi, Siti Habsah
author_sort	Roowi, Siti Habsah
title	Agrobacterium-mediated transformation of oil palm (Elaeis guineensis jacq.) suspension culture
title_short	Agrobacterium-mediated transformation of oil palm (Elaeis guineensis jacq.) suspension culture
title_full	Agrobacterium-mediated transformation of oil palm (Elaeis guineensis jacq.) suspension culture
title_fullStr	Agrobacterium-mediated transformation of oil palm (Elaeis guineensis jacq.) suspension culture
title_full_unstemmed	Agrobacterium-mediated transformation of oil palm (Elaeis guineensis jacq.) suspension culture
title_sort	agrobacterium-mediated transformation of oil palm (elaeis guineensis jacq.) suspension culture
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Food Science and Technology
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/8726/1/FSMB_2003_19%20IR.pdf
_version_	1794018777568903168

Agrobacterium-mediated transformation of oil palm (Elaeis guineensis jacq.) suspension culture

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