Optimization of Malaysia rice MR219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition

Rice is the staple food for a majority of the population around the world. Drought is one of the many factors that affect rice production yield. Researches have used various methods to develop suitable indicator in rice to defend the crop against drought environment. Among the biotechnology techniqu...

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Main Author:	Er, Choon Yeong
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Rice - Effect of stress on Rice Cell suspensions
Online Access:	http://psasir.upm.edu.my/id/eprint/90692/1/FBSB%202020%205%20-%20ir.pdf
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spelling	my-upm-ir.906922021-09-03T01:13:14Z Optimization of Malaysia rice MR219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition 2019-10 Er, Choon Yeong Rice is the staple food for a majority of the population around the world. Drought is one of the many factors that affect rice production yield. Researches have used various methods to develop suitable indicator in rice to defend the crop against drought environment. Among the biotechnology techniques, cell culture is commonly used to develop drought tolerant cell line. In Malaysia, drought has greatly affected rice production; as water is the essential element for rice and the heavy dependence of rice on irrigation will make rice as the crop most affected by water shortage crisis. Hence, it is a need to develop drought-tolerant rice in order to fulfil rice demand in Malaysia.The aim of this research is to investigate the biochemical responses in Malaysian rice by using plant cell culturing technique under water-limited condition. The cell suspension of Malaysian rice MR219 line 4 was established and it was cultured in vitro with drought agent (PEG6000) in order to prevent water up take by plant cell. After that, biochemical analyses were performed such as glyoxalase I (Gly I) enzyme assay, methylglyoxal, antioxidant enzymes activities – superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and lipid peroxidation of PEG6000 treated cultures were determined. The results showed that while PEG6000 concentration increased from 0%, 5%, 10%, 15% to 20%, the settle cell volume (SCV), cell viability and relative water content (RWC) were declined maximum to 115%, 34% and 24% respectively as a result of drought condition. When drought stress occurred, it eventually activated the plant cell defensive system by upregulating antioxidant enzymes activities (SOD, CAT and APX) to overcome oxidative injury under drought stress condition. It exhibited the highest relative activities for SOD (13.7708 U / mg proteins), CAT (0.1238 μmoL / mg protein / min) and APX (0.4852 μmoL / mg protein / min). Same trend has been applied to Gly I (4.4633 μmoL / mg protein / min). It implicated that the Gly I could be one of the potential drought stress indicator for rice. The output of this study would contribute tremendously to exploit potential drought-tolerant rice cultivar in the future. Rice - Effect of stress on Rice Cell suspensions 2019-10 Thesis http://psasir.upm.edu.my/id/eprint/90692/ http://psasir.upm.edu.my/id/eprint/90692/1/FBSB%202020%205%20-%20ir.pdf text en public masters Universiti Putra Malaysia Rice - Effect of stress on Rice Cell suspensions Shaharuddin, Noor Azmi
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Shaharuddin, Noor Azmi
topic	Rice - Effect of stress on Rice Cell suspensions
spellingShingle	Rice - Effect of stress on Rice Cell suspensions Er, Choon Yeong Optimization of Malaysia rice MR219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition
description	Rice is the staple food for a majority of the population around the world. Drought is one of the many factors that affect rice production yield. Researches have used various methods to develop suitable indicator in rice to defend the crop against drought environment. Among the biotechnology techniques, cell culture is commonly used to develop drought tolerant cell line. In Malaysia, drought has greatly affected rice production; as water is the essential element for rice and the heavy dependence of rice on irrigation will make rice as the crop most affected by water shortage crisis. Hence, it is a need to develop drought-tolerant rice in order to fulfil rice demand in Malaysia.The aim of this research is to investigate the biochemical responses in Malaysian rice by using plant cell culturing technique under water-limited condition. The cell suspension of Malaysian rice MR219 line 4 was established and it was cultured in vitro with drought agent (PEG6000) in order to prevent water up take by plant cell. After that, biochemical analyses were performed such as glyoxalase I (Gly I) enzyme assay, methylglyoxal, antioxidant enzymes activities – superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and lipid peroxidation of PEG6000 treated cultures were determined. The results showed that while PEG6000 concentration increased from 0%, 5%, 10%, 15% to 20%, the settle cell volume (SCV), cell viability and relative water content (RWC) were declined maximum to 115%, 34% and 24% respectively as a result of drought condition. When drought stress occurred, it eventually activated the plant cell defensive system by upregulating antioxidant enzymes activities (SOD, CAT and APX) to overcome oxidative injury under drought stress condition. It exhibited the highest relative activities for SOD (13.7708 U / mg proteins), CAT (0.1238 μmoL / mg protein / min) and APX (0.4852 μmoL / mg protein / min). Same trend has been applied to Gly I (4.4633 μmoL / mg protein / min). It implicated that the Gly I could be one of the potential drought stress indicator for rice. The output of this study would contribute tremendously to exploit potential drought-tolerant rice cultivar in the future.
format	Thesis
qualification_level	Master's degree
author	Er, Choon Yeong
author_facet	Er, Choon Yeong
author_sort	Er, Choon Yeong
title	Optimization of Malaysia rice MR219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition
title_short	Optimization of Malaysia rice MR219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition
title_full	Optimization of Malaysia rice MR219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition
title_fullStr	Optimization of Malaysia rice MR219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition
title_full_unstemmed	Optimization of Malaysia rice MR219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition
title_sort	optimization of malaysia rice mr219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition
granting_institution	Universiti Putra Malaysia
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/90692/1/FBSB%202020%205%20-%20ir.pdf
_version_	1747813647822880768

Optimization of Malaysia rice MR219 derivatives cell suspension culture and responses of stress-related enzymes under drought condition

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