Antibicrobial and cytotoxic compounds of Scorodocarpus borneensis (Olacaceae) and Glycosmis calcicola (Rutaceae)
The extracts of plants collected from the forest of Northern Malay Peninsula were tested against bacteria, fungi and CEM-SS leukemia cel1 line. Gram-positive Bacillus cereus and Gram-negative Pseudomonas aeruginosa which are commonly involved in skin infections, have been used for screening anti...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2001
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/9283/1/FSAS_2001_28_.pdf |
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| Summary: | The extracts of plants collected from the forest of Northern Malay
Peninsula were tested against bacteria, fungi and CEM-SS leukemia cel1 line.
Gram-positive Bacillus cereus and Gram-negative Pseudomonas aeruginosa which
are commonly involved in skin infections, have been used for screening
antibacterial activity whereas Candida lipolytica, Saccharomyces cerevisae,
Saccharomyces lipolytica, and Aspergillus ochraceous have been used for
screening antifungal activity.
The crude extract from the seeds of Scorodocarpus bomeensis showed a
strong cytotoxic activity against CEM-SS leukemia cell-line and antimicrobial
activities. A bioassay-guided separation had been carried out to separate the active
constituents of the seeds. This resulted in the isolation and identification of an
aliphatic sulfur compound, bis-(methylthiomethyl)disulfide, and a new indole alkalOld, 13-docosenoyl serotonme from the petroleum ether extract A new
sesquiterpene, scopotin, has been isolated from the chloroform extract of the seeds
Another sesquiterpene, cadalene-p-carboxyhc acid, has been isolated from the bark
of this species
bis-(Methyithiomethyl)-disulfide appeared as the most active compound
and the major constttuent of the seeds extract of Scorodocarpus borneensis it
acted significantly on a methicillin resistant strain of Staphylococcus aureus and
showed a strong Cytotoxic activity against CEM-SS leukemia and KU812F chronic
myelogenous leukemia cell lines 13-Docosenoyl serotonme showed a moderate
Cytotoxic activity against CEM-SS leukemia cell line Scopotin showed a moderate
antimicrobial activity and a strong Cytotixic effect against CEM-SS leukemia cell line
The crude petroleum ether extract and bis-(methyithtomethyl)disulfide
strongly inhibited the growth of pathogen fungi and was formulated m external
preparation by usmg commercial paraffin as exipient. This preparation exhibited
drastic antifungal activities in vitro and in VIVO The acute toxicity of the crude
petroleum ether extract was found to be inferior to phenobarbital in mice with
LD50 at 275 mg/kg The skin irritancy test was performed on rabbits and it showed
that this preparatlOn had an irritancy level acceptable by the FDA. Antimicrobial and cytotoxic activities were also performed on
methylgerambullin and desmethoxyzanthophylline which were previously isolated
from Glycosmis calcicola (Rutaceae). Methylgerambullin showed a strong
cytotoxic activity against CEM-SS leukemia, melanoma HACCl, and colon cancer
HT29 cell lines whereas desmethoxyzanthophylline has moderate activity against
Bacillus cereus, Pseudomonas aeruginosa and CEM-SS leukemia cell line. |
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