Antioxidant and antidiabetic activity of phenolic compounds extracted from Orthosiphon stamineus extracts

Orthosiphon stamineus Benth (Lamiaceae), locally called Misai Kucing, is a local herb that grows widely in Malaysia. It is gaining popularity and well-known plant in herbal medicine. The objective of this study is to identified and quantified the antioxidant activity and phenolic compounds using HPL...

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Main Author:	Abdul Mubdi, Nurazrin Ezrina
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Herbs - Therapeutic use Lamiaceae Medicinal plants
Online Access:	http://psasir.upm.edu.my/id/eprint/93099/1/FBSB%202020%2026%20IR.pdf
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id	my-upm-ir.93099
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spelling	my-upm-ir.930992022-06-07T06:29:18Z Antioxidant and antidiabetic activity of phenolic compounds extracted from Orthosiphon stamineus extracts 2019-05 Abdul Mubdi, Nurazrin Ezrina Orthosiphon stamineus Benth (Lamiaceae), locally called Misai Kucing, is a local herb that grows widely in Malaysia. It is gaining popularity and well-known plant in herbal medicine. The objective of this study is to identified and quantified the antioxidant activity and phenolic compounds using HPLC, HPLC-PDA-MS2 and HPLC with post-column on-line antioxidant detection based on 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay. The results had shown the phytochemicals in water extracts contained salvinalic acid, chlorogenic acid, caffeic acid and rosmarinic acid. Salvinalic acid and caffeic acid in O. stamineus extracts possess high Trolox Equivalent Antioxidant Ratio (TEAR) values by 0.74 and 0.50 respectively. O. stamineus has been scientifically proven to have hypoglycaemic and antihyperglycaemic activity. In this study, hypoglycaemic and antihyperglycaemic activity of O. stamineus water extracts in normal and diabetic rats (streptozotocin induced) was evaluated. In evaluation of hypoglycaemic activity of O. stamineus extracts in normal rats, medium doses (15 mg/kg) and high doses (30 mg/kg) significantly reduced blood glucose level to 4.5 mmol/L and 4.14 mmol/L after 6 (p ˂ 0.05) hours extracts administration. Metformin, 500 mg/kg also shows hypoglycaemic activity after 6 hours administration. The evaluation of antihyperglycaemic activity, the results showed that O. stamineus extracts significantly reduced fasting and postprandial hyperglycaemic particularly after 2 and 4 hours extracts administration at high doses (30 mg/kg). In conclusion, the usage of analytical instruments such as HPLC, HPLC-PDA-MS2 and antioxidant assays has prompted to identification of phenolic compounds and antioxidant activities in Malaysian traditional herbs. This study showed that O. stamineus water extract can be employed and suitable as dietary subordinate to deal with hyperglycemia in diabetes patients. Herbs - Therapeutic use Lamiaceae Medicinal plants 2019-05 Thesis http://psasir.upm.edu.my/id/eprint/93099/ http://psasir.upm.edu.my/id/eprint/93099/1/FBSB%202020%2026%20IR.pdf text en public masters Universiti Putra Malaysia Herbs - Therapeutic use Lamiaceae Medicinal plants Hamid, Muhajir
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Hamid, Muhajir
topic	Herbs - Therapeutic use Lamiaceae Medicinal plants
spellingShingle	Herbs - Therapeutic use Lamiaceae Medicinal plants Abdul Mubdi, Nurazrin Ezrina Antioxidant and antidiabetic activity of phenolic compounds extracted from Orthosiphon stamineus extracts
description	Orthosiphon stamineus Benth (Lamiaceae), locally called Misai Kucing, is a local herb that grows widely in Malaysia. It is gaining popularity and well-known plant in herbal medicine. The objective of this study is to identified and quantified the antioxidant activity and phenolic compounds using HPLC, HPLC-PDA-MS2 and HPLC with post-column on-line antioxidant detection based on 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay. The results had shown the phytochemicals in water extracts contained salvinalic acid, chlorogenic acid, caffeic acid and rosmarinic acid. Salvinalic acid and caffeic acid in O. stamineus extracts possess high Trolox Equivalent Antioxidant Ratio (TEAR) values by 0.74 and 0.50 respectively. O. stamineus has been scientifically proven to have hypoglycaemic and antihyperglycaemic activity. In this study, hypoglycaemic and antihyperglycaemic activity of O. stamineus water extracts in normal and diabetic rats (streptozotocin induced) was evaluated. In evaluation of hypoglycaemic activity of O. stamineus extracts in normal rats, medium doses (15 mg/kg) and high doses (30 mg/kg) significantly reduced blood glucose level to 4.5 mmol/L and 4.14 mmol/L after 6 (p ˂ 0.05) hours extracts administration. Metformin, 500 mg/kg also shows hypoglycaemic activity after 6 hours administration. The evaluation of antihyperglycaemic activity, the results showed that O. stamineus extracts significantly reduced fasting and postprandial hyperglycaemic particularly after 2 and 4 hours extracts administration at high doses (30 mg/kg). In conclusion, the usage of analytical instruments such as HPLC, HPLC-PDA-MS2 and antioxidant assays has prompted to identification of phenolic compounds and antioxidant activities in Malaysian traditional herbs. This study showed that O. stamineus water extract can be employed and suitable as dietary subordinate to deal with hyperglycemia in diabetes patients.
format	Thesis
qualification_level	Master's degree
author	Abdul Mubdi, Nurazrin Ezrina
author_facet	Abdul Mubdi, Nurazrin Ezrina
author_sort	Abdul Mubdi, Nurazrin Ezrina
title	Antioxidant and antidiabetic activity of phenolic compounds extracted from Orthosiphon stamineus extracts
title_short	Antioxidant and antidiabetic activity of phenolic compounds extracted from Orthosiphon stamineus extracts
title_full	Antioxidant and antidiabetic activity of phenolic compounds extracted from Orthosiphon stamineus extracts
title_fullStr	Antioxidant and antidiabetic activity of phenolic compounds extracted from Orthosiphon stamineus extracts
title_full_unstemmed	Antioxidant and antidiabetic activity of phenolic compounds extracted from Orthosiphon stamineus extracts
title_sort	antioxidant and antidiabetic activity of phenolic compounds extracted from orthosiphon stamineus extracts
granting_institution	Universiti Putra Malaysia
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/93099/1/FBSB%202020%2026%20IR.pdf
_version_	1747813788453699584

Antioxidant and antidiabetic activity of phenolic compounds extracted from Orthosiphon stamineus extracts

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