Lipase-Catalyzed Synthesis of Wax Ester
Alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme and Novozyme was carried out to produce oleyl oleate, a wax ester. Several reaction parameters were studied in order to get its optimal condition such as effect of time, reaction temperature, amount of enzyme, molar rat...
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2001
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my-upm-ir.93212024-02-20T01:31:59Z Lipase-Catalyzed Synthesis of Wax Ester 2001-01 Mat Hadzir, Nursyamsyila Alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme and Novozyme was carried out to produce oleyl oleate, a wax ester. Several reaction parameters were studied in order to get its optimal condition such as effect of time, reaction temperature, amount of enzyme, molar ratio of substrates (oleyl alcohol/trioleib), various organic solvents used, initial water activity (aw), continuously controlled water activity and addition of silica gel. The optimal condition to produce wax ester for Lipozyme are incubation time of 5 h, temperature of 50°c and using 30% (w/v) of Lipozyme. The molar ratio of substmte was 6:1 (oleyl alcohol/triolein) in heptane and at aw 0.33 (initial water activity) or 0.32 (continuously controlled water activity) with the addition of 0.25 g of silica gel. For Novozyme, the optimum incubation time was 5 h, at reaction temperature of 60 °c using 30% (w/v) of Novozyme. The optimum molar ratio of substrates (oleyl alcohol/triolein) was also at 6:1, in heptane at aw 0.33 (initial water activity, aw), 0.32 (continuously controlled water activity) and with the addition of 0.50 g of silica gel. A scale up reaction was then carried out using the optimum condition catalyzed by Lipozyme. The percentage yield of wax ester produced at optimum reaction condition was 58.48% when determined by gravimetry method and 75.66% when determined using gas chromatography. Lipase - Synthesis Catalysts 2001-01 Thesis http://psasir.upm.edu.my/id/eprint/9321/ http://psasir.upm.edu.my/id/eprint/9321/1/FSAS_2001_54.pdf text en public masters Universiti Putra Malaysia Lipase - Synthesis Catalysts Faculty of Science and Environmental Studies Basri, Mahiran English |
| institution |
Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English English |
| advisor |
Basri, Mahiran |
| topic |
Lipase - Synthesis Catalysts |
| spellingShingle |
Lipase - Synthesis Catalysts Mat Hadzir, Nursyamsyila Lipase-Catalyzed Synthesis of Wax Ester |
| description |
Alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme
and Novozyme was carried out to produce oleyl oleate, a wax ester. Several reaction
parameters were studied in order to get its optimal condition such as effect of time,
reaction temperature, amount of enzyme, molar ratio of substrates (oleyl alcohol/trioleib),
various organic solvents used, initial water activity (aw), continuously controlled water
activity and addition of silica gel.
The optimal condition to produce wax ester for Lipozyme are incubation time of
5 h, temperature of 50°c and using 30% (w/v) of Lipozyme. The molar ratio of substmte
was 6:1 (oleyl alcohol/triolein) in heptane and at aw 0.33 (initial water activity) or 0.32
(continuously controlled water activity) with the addition of 0.25 g of silica gel. For
Novozyme, the optimum incubation time was 5 h, at reaction temperature of 60 °c using
30% (w/v) of Novozyme. The optimum molar ratio of substrates (oleyl alcohol/triolein) was also at 6:1, in heptane at aw 0.33 (initial water activity, aw), 0.32 (continuously
controlled water activity) and with the addition of 0.50 g of silica gel.
A scale up reaction was then carried out using the optimum condition catalyzed
by Lipozyme. The percentage yield of wax ester produced at optimum reaction condition
was 58.48% when determined by gravimetry method and 75.66% when determined using
gas chromatography. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Mat Hadzir, Nursyamsyila |
| author_facet |
Mat Hadzir, Nursyamsyila |
| author_sort |
Mat Hadzir, Nursyamsyila |
| title |
Lipase-Catalyzed Synthesis of Wax Ester |
| title_short |
Lipase-Catalyzed Synthesis of Wax Ester |
| title_full |
Lipase-Catalyzed Synthesis of Wax Ester |
| title_fullStr |
Lipase-Catalyzed Synthesis of Wax Ester |
| title_full_unstemmed |
Lipase-Catalyzed Synthesis of Wax Ester |
| title_sort |
lipase-catalyzed synthesis of wax ester |
| granting_institution |
Universiti Putra Malaysia |
| granting_department |
Faculty of Science and Environmental Studies |
| publishDate |
2001 |
| url |
http://psasir.upm.edu.my/id/eprint/9321/1/FSAS_2001_54.pdf |
| _version_ |
1794018821893259264 |