Genetic Studies and Growth Performance of the Yellow Catfish, Mystus Nemurus (Cuv. & Val.) in Thailand
Yellow catfish, Mystus nemurus, was examined to determine levels of genetic subpopulation differentiation among samples of this species obtained from different parts of its range, as well as to compare the genetics of wild and hatchery-bred fish. Horizontal starch gel electrophoresis and histoche...
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2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/9363/1/FSAS_2002_8.pdf |
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| advisor |
Siraj, Siti Shapor |
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Flathead catfish - Genetics - Thailand. Flathead catfish - Growth - Thailand. |
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Flathead catfish - Genetics - Thailand. Flathead catfish - Growth - Thailand. Leesanga, Sanga Genetic Studies and Growth Performance of the Yellow Catfish, Mystus Nemurus (Cuv. & Val.) in Thailand |
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Yellow catfish, Mystus nemurus, was examined to determine levels of genetic subpopulation
differentiation among samples of this species obtained from different parts
of its range, as well as to compare the genetics of wild and hatchery-bred fish.
Horizontal starch gel electrophoresis and histochemical staining techniques were used
to examine genetic variation within and among 8 wild and one hatchery populations of
M. nemurus in Thailand. Individual specimens were analyzed. at 23 protein-coding
loci. Fifteen of the 23 loci examined (65.22 %) were polymorphic. Observed
heterozygosities ranged from 0.041 to 0.111. Genetic distance estimates ranged from
0.00510 0.l64. The greatest genetic distance was found between the Chainat and
Suratthani populations (0.164), which probably, a level indicative of subspecific
differentiation in M. nemurus from within Thailand. Eight wild populations collected from throughout Thailand and a hatchery stock of M.
nemurus were also analyzed at the molecular (DNA) level using the technique of
RAPD-PCR fingerprinting. Five arbitrary primers were chosen to amplify products
which showed 28 polymorphic loci (60.87 %). The highest genetic distance (D) was
found between the Chainat and Suratthani populations with a value of 0.289, whereas
the lowest was found in the pair of Songkhla population and hatchery stock with a
value of 0.087. The dendrogram depicts the genetic relationships among populations
of M. nemurus which grouped into four clusters according to their regions of origin
namely northern group (Chiengrai and Sukhothai), central group (Kanchanaburi and
Chainat), north-eastern group (Nongkhai and Nakormpanom) and southern group
(Suratthani, Songkbla and hatchery stock).
Horizontal starch gel electrophoresis was applied to differentiate between Mystid and
Tachysurid species of catfish : Mystus nemurus, M cavasius, M gulio, Tachysurus
caelatus and T. truncatus, collected from the Tapee River, Thailand. Individual fishes
were analysed for 22 enzymatic loci. Eleven loci (50.00 %) were found to be
polymorphic (P<0.95). The percentage of polymorphic loci ranged from 14.3 % in
T. caelatus to 38.1 % in M. nemurus. The highest mean heterozygosity was found in
T. truncatus (0. 121±0.048) and the lowest in M cavasius (0.026±0.014). Genetic
distance estimates among Mystus species ranged from 0.167 to 0.364 while genetic
distance was 0.359 between the two species of Tachysurus. The genetic distance
estimates between Mystus and Tachysurus populations ranged from 0.693 to 0.878. Morphometric and meristic variation among two populations from Chainat and
Suratthani, which showed the highest genetic distance based on both the isozyme and
DNA data, were studied. From the 23 morphometric characters examined, 20
characters showed significant differences. Most characters from the Chainat
population differed from the Suratthani population especially for eye diameter (ED)
and the distance from the posterior end of the dorsal fin to the anterior end of the
adipose fin (DDA), which seemed to be consistent diagnostic characters
differentiating the two populations.
The relative growth performance of M nemurus collected from northern (Uttaradith),
central (Chainat), and southern Thailand (Suratthani) were investigated. Three
populations of fish broodstocks were induced to spawn at the same time. The fish
larvae were nursed in 0.5 x 2.0 x 0.2 m tanks for 8 weeks. After nursing the
fingerlings were separated into 2 groups and cultured at a stocking density of 50
fish/cage in l.0 x 1.0 x 1.5 m floating net cages, in which one group was placed in the
north (Lumpang) and another group in the south of Thailand (Suratthani). Fish were
fed to satiation with 30 % protein pellets for 48 weeks. At the end of the experiment,
the growth performance of fish from both locations (north and south Thailand) were
taken and analyzed. The highest growth was found in the population from the south,
whereas the lowest growth was found in the central population. The results are in
accordance with the values of genetic distances based on both isozyme and RAPD data. |
| format |
Thesis |
| qualification_level |
Doctorate |
| author |
Leesanga, Sanga |
| author_facet |
Leesanga, Sanga |
| author_sort |
Leesanga, Sanga |
| title |
Genetic Studies and Growth Performance of the Yellow Catfish, Mystus Nemurus (Cuv. & Val.) in Thailand |
| title_short |
Genetic Studies and Growth Performance of the Yellow Catfish, Mystus Nemurus (Cuv. & Val.) in Thailand |
| title_full |
Genetic Studies and Growth Performance of the Yellow Catfish, Mystus Nemurus (Cuv. & Val.) in Thailand |
| title_fullStr |
Genetic Studies and Growth Performance of the Yellow Catfish, Mystus Nemurus (Cuv. & Val.) in Thailand |
| title_full_unstemmed |
Genetic Studies and Growth Performance of the Yellow Catfish, Mystus Nemurus (Cuv. & Val.) in Thailand |
| title_sort |
genetic studies and growth performance of the yellow catfish, mystus nemurus (cuv. & val.) in thailand |
| granting_institution |
Universiti Putra Malaysia |
| granting_department |
Faculty of Science and Environmental Studies |
| publishDate |
2002 |
| url |
http://psasir.upm.edu.my/id/eprint/9363/1/FSAS_2002_8.pdf |
| _version_ |
1794018829344440320 |
| spelling |
my-upm-ir.93632024-02-21T01:40:54Z Genetic Studies and Growth Performance of the Yellow Catfish, Mystus Nemurus (Cuv. & Val.) in Thailand 2002-04 Leesanga, Sanga Yellow catfish, Mystus nemurus, was examined to determine levels of genetic subpopulation differentiation among samples of this species obtained from different parts of its range, as well as to compare the genetics of wild and hatchery-bred fish. Horizontal starch gel electrophoresis and histochemical staining techniques were used to examine genetic variation within and among 8 wild and one hatchery populations of M. nemurus in Thailand. Individual specimens were analyzed. at 23 protein-coding loci. Fifteen of the 23 loci examined (65.22 %) were polymorphic. Observed heterozygosities ranged from 0.041 to 0.111. Genetic distance estimates ranged from 0.00510 0.l64. The greatest genetic distance was found between the Chainat and Suratthani populations (0.164), which probably, a level indicative of subspecific differentiation in M. nemurus from within Thailand. Eight wild populations collected from throughout Thailand and a hatchery stock of M. nemurus were also analyzed at the molecular (DNA) level using the technique of RAPD-PCR fingerprinting. Five arbitrary primers were chosen to amplify products which showed 28 polymorphic loci (60.87 %). The highest genetic distance (D) was found between the Chainat and Suratthani populations with a value of 0.289, whereas the lowest was found in the pair of Songkhla population and hatchery stock with a value of 0.087. The dendrogram depicts the genetic relationships among populations of M. nemurus which grouped into four clusters according to their regions of origin namely northern group (Chiengrai and Sukhothai), central group (Kanchanaburi and Chainat), north-eastern group (Nongkhai and Nakormpanom) and southern group (Suratthani, Songkbla and hatchery stock). Horizontal starch gel electrophoresis was applied to differentiate between Mystid and Tachysurid species of catfish : Mystus nemurus, M cavasius, M gulio, Tachysurus caelatus and T. truncatus, collected from the Tapee River, Thailand. Individual fishes were analysed for 22 enzymatic loci. Eleven loci (50.00 %) were found to be polymorphic (P<0.95). The percentage of polymorphic loci ranged from 14.3 % in T. caelatus to 38.1 % in M. nemurus. The highest mean heterozygosity was found in T. truncatus (0. 121±0.048) and the lowest in M cavasius (0.026±0.014). Genetic distance estimates among Mystus species ranged from 0.167 to 0.364 while genetic distance was 0.359 between the two species of Tachysurus. The genetic distance estimates between Mystus and Tachysurus populations ranged from 0.693 to 0.878. Morphometric and meristic variation among two populations from Chainat and Suratthani, which showed the highest genetic distance based on both the isozyme and DNA data, were studied. From the 23 morphometric characters examined, 20 characters showed significant differences. Most characters from the Chainat population differed from the Suratthani population especially for eye diameter (ED) and the distance from the posterior end of the dorsal fin to the anterior end of the adipose fin (DDA), which seemed to be consistent diagnostic characters differentiating the two populations. The relative growth performance of M nemurus collected from northern (Uttaradith), central (Chainat), and southern Thailand (Suratthani) were investigated. Three populations of fish broodstocks were induced to spawn at the same time. The fish larvae were nursed in 0.5 x 2.0 x 0.2 m tanks for 8 weeks. After nursing the fingerlings were separated into 2 groups and cultured at a stocking density of 50 fish/cage in l.0 x 1.0 x 1.5 m floating net cages, in which one group was placed in the north (Lumpang) and another group in the south of Thailand (Suratthani). Fish were fed to satiation with 30 % protein pellets for 48 weeks. At the end of the experiment, the growth performance of fish from both locations (north and south Thailand) were taken and analyzed. The highest growth was found in the population from the south, whereas the lowest growth was found in the central population. The results are in accordance with the values of genetic distances based on both isozyme and RAPD data. Flathead catfish - Genetics - Thailand. Flathead catfish - Growth - Thailand. 2002-04 Thesis http://psasir.upm.edu.my/id/eprint/9363/ http://psasir.upm.edu.my/id/eprint/9363/1/FSAS_2002_8.pdf text en public doctoral Universiti Putra Malaysia Flathead catfish - Genetics - Thailand. Flathead catfish - Growth - Thailand. Faculty of Science and Environmental Studies Siraj, Siti Shapor English |