Determination of Biological Species of Ganoderma Boninense (Pat.) and Their Pathogenic Potential on Oil Palm (Elaeis Guineensis Jacq.) Seedlings

A total of 5 Ganoderma isolates from oil palm (Elaeis guineensis Jacq.) and coconut (Cocos nucifera L.) hosts were used in the determination of Ganoderma boninense based on monokaryotic mating compatibilities. Ganoderma specimens collected from both oil palm and coconut hosts fell into 2 types; o...

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Main Author:	Malik Habib, Nelson
Format:	Thesis
Language:	English English
Published:	2002
Subjects:	Pathogenic microorganisms Ganoderma diseases of plants
Online Access:	http://psasir.upm.edu.my/id/eprint/9381/1/FSAS_2002_16.pdf
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id	my-upm-ir.9381
record_format	uketd_dc
spelling	my-upm-ir.93812024-02-21T04:24:43Z Determination of Biological Species of Ganoderma Boninense (Pat.) and Their Pathogenic Potential on Oil Palm (Elaeis Guineensis Jacq.) Seedlings 2002-02 Malik Habib, Nelson A total of 5 Ganoderma isolates from oil palm (Elaeis guineensis Jacq.) and coconut (Cocos nucifera L.) hosts were used in the determination of Ganoderma boninense based on monokaryotic mating compatibilities. Ganoderma specimens collected from both oil palm and coconut hosts fell into 2 types; one was reddish brown with a varnished dorsal surface ('laccate') represented by isolates EGB-01, CN-Ll and CN-L2 and the other a dull brown non-varnished dorsal surface ('non laccate') represented by EG-NL and CN-NL. The isolate WD814 identified as G. boninense by the Forestry and Forest Products Research Institute (FFRRI) at Tsukuba, Japan, was included as an outgroup sample. The present study showed that based on sexual compatibilities of monosporus cultures obtained from a single fruitbody, the mating pattern of G. boninense was heterothallic and tetrapolar. The cultures fell into 4 distinct sex groups from which 4 monokaryon testers were successfully obtained. When tested for intergroup compatibilities, isolates CN-Ll and WD814 were compatible with EGB-Ol, whereas CN-L2, EG-NL and CN-NL were not. Thus. monokaryon compatibility crosses confirmed that although EGB-01, CN-L 1 and WD814 were from oil palm, coconut and Livistona palm hosts respectively, they all belonged to the same biological species. The pathogenic potential of isolates from oil palm and coconut hosts were tested on oil palm seedlings over a 12-month period in glasshouse trials. Only EGB- 01 and CN-Ll isolates were found to be pathogenic on oil palm seedlings, with both showing 100% infection. Totally no infection was established in palms inoculated with the non-G. boninense isolates EG-NL, CN-NL and CN-L2. When infected seedlings were uprooted, the point of entry of G. boninenese was found to be the larger primary root of the plant. A scanning electron micrograph (SEM) of a newly infected root showed that the fungi colonised the epidermis, cortex, endodermis, lacunae, as well as plugged the xylem and phloem tissues. This indicates that fungal colonisation was not restricted to any particular tissue. This study concluded firstly, more than one species of laccate Ganoderma can be found on coconut stumps. Secondly, G. boninense isolated from oil palm and coconut stumps were pathogenic on oil palms. Thirdly, the non G. boninense specimens (EG-NL, CN-NL and CNL2) were not pathogenic to oil palms. Pathogenic microorganisms Ganoderma diseases of plants 2002-02 Thesis http://psasir.upm.edu.my/id/eprint/9381/ http://psasir.upm.edu.my/id/eprint/9381/1/FSAS_2002_16.pdf text en public masters Universiti Putra Malaysia Pathogenic microorganisms Ganoderma diseases of plants Faculty of Science and Environmental Studies Abdullah, Faridah English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Abdullah, Faridah
topic	Pathogenic microorganisms Ganoderma diseases of plants
spellingShingle	Pathogenic microorganisms Ganoderma diseases of plants Malik Habib, Nelson Determination of Biological Species of Ganoderma Boninense (Pat.) and Their Pathogenic Potential on Oil Palm (Elaeis Guineensis Jacq.) Seedlings
description	A total of 5 Ganoderma isolates from oil palm (Elaeis guineensis Jacq.) and coconut (Cocos nucifera L.) hosts were used in the determination of Ganoderma boninense based on monokaryotic mating compatibilities. Ganoderma specimens collected from both oil palm and coconut hosts fell into 2 types; one was reddish brown with a varnished dorsal surface ('laccate') represented by isolates EGB-01, CN-Ll and CN-L2 and the other a dull brown non-varnished dorsal surface ('non laccate') represented by EG-NL and CN-NL. The isolate WD814 identified as G. boninense by the Forestry and Forest Products Research Institute (FFRRI) at Tsukuba, Japan, was included as an outgroup sample. The present study showed that based on sexual compatibilities of monosporus cultures obtained from a single fruitbody, the mating pattern of G. boninense was heterothallic and tetrapolar. The cultures fell into 4 distinct sex groups from which 4 monokaryon testers were successfully obtained. When tested for intergroup compatibilities, isolates CN-Ll and WD814 were compatible with EGB-Ol, whereas CN-L2, EG-NL and CN-NL were not. Thus. monokaryon compatibility crosses confirmed that although EGB-01, CN-L 1 and WD814 were from oil palm, coconut and Livistona palm hosts respectively, they all belonged to the same biological species. The pathogenic potential of isolates from oil palm and coconut hosts were tested on oil palm seedlings over a 12-month period in glasshouse trials. Only EGB- 01 and CN-Ll isolates were found to be pathogenic on oil palm seedlings, with both showing 100% infection. Totally no infection was established in palms inoculated with the non-G. boninense isolates EG-NL, CN-NL and CN-L2. When infected seedlings were uprooted, the point of entry of G. boninenese was found to be the larger primary root of the plant. A scanning electron micrograph (SEM) of a newly infected root showed that the fungi colonised the epidermis, cortex, endodermis, lacunae, as well as plugged the xylem and phloem tissues. This indicates that fungal colonisation was not restricted to any particular tissue. This study concluded firstly, more than one species of laccate Ganoderma can be found on coconut stumps. Secondly, G. boninense isolated from oil palm and coconut stumps were pathogenic on oil palms. Thirdly, the non G. boninense specimens (EG-NL, CN-NL and CNL2) were not pathogenic to oil palms.
format	Thesis
qualification_level	Master's degree
author	Malik Habib, Nelson
author_facet	Malik Habib, Nelson
author_sort	Malik Habib, Nelson
title	Determination of Biological Species of Ganoderma Boninense (Pat.) and Their Pathogenic Potential on Oil Palm (Elaeis Guineensis Jacq.) Seedlings
title_short	Determination of Biological Species of Ganoderma Boninense (Pat.) and Their Pathogenic Potential on Oil Palm (Elaeis Guineensis Jacq.) Seedlings
title_full	Determination of Biological Species of Ganoderma Boninense (Pat.) and Their Pathogenic Potential on Oil Palm (Elaeis Guineensis Jacq.) Seedlings
title_fullStr	Determination of Biological Species of Ganoderma Boninense (Pat.) and Their Pathogenic Potential on Oil Palm (Elaeis Guineensis Jacq.) Seedlings
title_full_unstemmed	Determination of Biological Species of Ganoderma Boninense (Pat.) and Their Pathogenic Potential on Oil Palm (Elaeis Guineensis Jacq.) Seedlings
title_sort	determination of biological species of ganoderma boninense (pat.) and their pathogenic potential on oil palm (elaeis guineensis jacq.) seedlings
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	2002
url	http://psasir.upm.edu.my/id/eprint/9381/1/FSAS_2002_16.pdf
_version_	1794018831026356224

Determination of Biological Species of Ganoderma Boninense (Pat.) and Their Pathogenic Potential on Oil Palm (Elaeis Guineensis Jacq.) Seedlings

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