Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP

The main objectives of this study are to evaluate the usefulness of a variety of recently developed DNA markers to identify and characterise different populations of Malaysian river catfish, Mystus numerus. In the initial methodology development, 111 primers comprising of 40 single RAPD primers,...

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Main Author:	Chong, Lee Kim
Format:	Thesis
Language:	English English
Published:	1998
Subjects:	DNA polymerases Bagre - Malaysia
Online Access:	http://psasir.upm.edu.my/id/eprint/9423/1/FSAS_1998_17_A.pdf
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id	my-upm-ir.9423
record_format	uketd_dc
spelling	my-upm-ir.94232024-02-29T08:21:35Z Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP 1998-11 Chong, Lee Kim The main objectives of this study are to evaluate the usefulness of a variety of recently developed DNA markers to identify and characterise different populations of Malaysian river catfish, Mystus numerus. In the initial methodology development, 111 primers comprising of 40 single RAPD primers, 64 pairs of AFLP primers and 7 pairs of African catfish microsatellite primers were screened against M. nemurus samples. RAPD and AFLP primers gave positive results. Both techniques were also successfully used in examining the genetic diversity present in five populations of M. nemurus originating from Kedah. Perak, Johor, Sarawak and one UPM culture population. However, none of the micro satellite primers developed for the African catfish was suitable for typing our local M. nemurus. Therefore, this technique was not used for the population studies and emphasis was only given to RAPD and AFLP markers in this thesis. Nine RAPD primers and four AFLP primers detected a total of 42 and 158 polymorphic markers respectively. The modes of inheritance of the bands produced by four out of the nine RAPD primers and two out of the four AFLP primers were studied using family samples. The results showed that 9 of the RAPD markers and 24 of the AFLP markers used in population studies segregated as stable Mendelian loci while 2 RAPD markers and 13 AFLP markers showed unusual segregation. The rest of the markers could not be examined because no segregation was found in the families. DNA polymerases Bagre - Malaysia 1998-11 Thesis http://psasir.upm.edu.my/id/eprint/9423/ http://psasir.upm.edu.my/id/eprint/9423/1/FSAS_1998_17_A.pdf text en public masters Universiti Putra Malaysia DNA polymerases Bagre - Malaysia Faculty of Science and Environmental Studies Tan, Soon Guan English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Tan, Soon Guan
topic	DNA polymerases Bagre - Malaysia
spellingShingle	DNA polymerases Bagre - Malaysia Chong, Lee Kim Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
description	The main objectives of this study are to evaluate the usefulness of a variety of recently developed DNA markers to identify and characterise different populations of Malaysian river catfish, Mystus numerus. In the initial methodology development, 111 primers comprising of 40 single RAPD primers, 64 pairs of AFLP primers and 7 pairs of African catfish microsatellite primers were screened against M. nemurus samples. RAPD and AFLP primers gave positive results. Both techniques were also successfully used in examining the genetic diversity present in five populations of M. nemurus originating from Kedah. Perak, Johor, Sarawak and one UPM culture population. However, none of the micro satellite primers developed for the African catfish was suitable for typing our local M. nemurus. Therefore, this technique was not used for the population studies and emphasis was only given to RAPD and AFLP markers in this thesis. Nine RAPD primers and four AFLP primers detected a total of 42 and 158 polymorphic markers respectively. The modes of inheritance of the bands produced by four out of the nine RAPD primers and two out of the four AFLP primers were studied using family samples. The results showed that 9 of the RAPD markers and 24 of the AFLP markers used in population studies segregated as stable Mendelian loci while 2 RAPD markers and 13 AFLP markers showed unusual segregation. The rest of the markers could not be examined because no segregation was found in the families.
format	Thesis
qualification_level	Master's degree
author	Chong, Lee Kim
author_facet	Chong, Lee Kim
author_sort	Chong, Lee Kim
title	Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_short	Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_full	Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_fullStr	Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_full_unstemmed	Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_sort	development of pcr-based dna markers to identify aand characterise malaysian river catfish, mystus nemurus (c&v) : rapd and aflp
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	1998
url	http://psasir.upm.edu.my/id/eprint/9423/1/FSAS_1998_17_A.pdf
_version_	1794018836575420416

Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP

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