Genetic Transformation of Orchid Dendrobium Sonia-17 Using the Biolistic Method

The ever-changing tastes and preferences of orchid consumers initiated the need to create new and better varieties. Progress in molecular biology has allowed genetically well defined characteristics to be added to the gene pools, thereby increasing the potential for genetic improvement. However,...

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Main Author:	Abdullah, Janna Ong
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Genetic transformation Dendrobium - Molecular aspects Plant molecular biology
Online Access:	http://psasir.upm.edu.my/id/eprint/9465/1/FSAS_2001_29_A.pdf
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id	my-upm-ir.9465
record_format	uketd_dc
spelling	my-upm-ir.94652024-03-06T01:41:25Z Genetic Transformation of Orchid Dendrobium Sonia-17 Using the Biolistic Method 2001-06 Abdullah, Janna Ong The ever-changing tastes and preferences of orchid consumers initiated the need to create new and better varieties. Progress in molecular biology has allowed genetically well defined characteristics to be added to the gene pools, thereby increasing the potential for genetic improvement. However, such effort at creating a custom-made flower has yet to be realised in orchids. The present study aims at developing a genetic transformation system for the introduction of specific foreign genes into orchid. Protocorm-like-bodies (PLBs) of orchid hybrid, Dendrobium Sonia-17, were established to be suitable target tissues for the introduction of foreign genes using the biolistic method. They were easily micropropagated in vitro that provided plenty of materials to work with and were a reliable source of potentially regenerabIe tissues. The effect of blasting on the growth of the PLBs was evaluated by subjecting the PLBs to bombardment with uncoated gold microparticles. One month following bombardment, fresh weights gained by the PLBs were recorded. The results showed that bombarded PLBs had higher weight increments compared to non-bombarded treatments, indicating that subsequent lethal responses by the PLBs on antibiotic selections were mainly due to the selection pressure and not as a result of injuries inflicted during the bombardment. Genetic transformation Dendrobium - Molecular aspects Plant molecular biology 2001-06 Thesis http://psasir.upm.edu.my/id/eprint/9465/ http://psasir.upm.edu.my/id/eprint/9465/1/FSAS_2001_29_A.pdf application/pdf en public doctoral Universiti Putra Malaysia Genetic transformation Dendrobium - Molecular aspects Plant molecular biology Faculty of Science and Environmental Studies Mahmood, Marziah English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Mahmood, Marziah
topic	Genetic transformation Dendrobium - Molecular aspects Plant molecular biology
spellingShingle	Genetic transformation Dendrobium - Molecular aspects Plant molecular biology Abdullah, Janna Ong Genetic Transformation of Orchid Dendrobium Sonia-17 Using the Biolistic Method
description	The ever-changing tastes and preferences of orchid consumers initiated the need to create new and better varieties. Progress in molecular biology has allowed genetically well defined characteristics to be added to the gene pools, thereby increasing the potential for genetic improvement. However, such effort at creating a custom-made flower has yet to be realised in orchids. The present study aims at developing a genetic transformation system for the introduction of specific foreign genes into orchid. Protocorm-like-bodies (PLBs) of orchid hybrid, Dendrobium Sonia-17, were established to be suitable target tissues for the introduction of foreign genes using the biolistic method. They were easily micropropagated in vitro that provided plenty of materials to work with and were a reliable source of potentially regenerabIe tissues. The effect of blasting on the growth of the PLBs was evaluated by subjecting the PLBs to bombardment with uncoated gold microparticles. One month following bombardment, fresh weights gained by the PLBs were recorded. The results showed that bombarded PLBs had higher weight increments compared to non-bombarded treatments, indicating that subsequent lethal responses by the PLBs on antibiotic selections were mainly due to the selection pressure and not as a result of injuries inflicted during the bombardment.
format	Thesis
qualification_level	Doctorate
author	Abdullah, Janna Ong
author_facet	Abdullah, Janna Ong
author_sort	Abdullah, Janna Ong
title	Genetic Transformation of Orchid Dendrobium Sonia-17 Using the Biolistic Method
title_short	Genetic Transformation of Orchid Dendrobium Sonia-17 Using the Biolistic Method
title_full	Genetic Transformation of Orchid Dendrobium Sonia-17 Using the Biolistic Method
title_fullStr	Genetic Transformation of Orchid Dendrobium Sonia-17 Using the Biolistic Method
title_full_unstemmed	Genetic Transformation of Orchid Dendrobium Sonia-17 Using the Biolistic Method
title_sort	genetic transformation of orchid dendrobium sonia-17 using the biolistic method
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/9465/1/FSAS_2001_29_A.pdf
_version_	1794018842876313600

Genetic Transformation of Orchid Dendrobium Sonia-17 Using the Biolistic Method

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