Chemical Constituents of Murraya Koenigii (Rutaceae) and Their Biological Activities
The study on Murraya koenigii (curry leaf) involves extraction, separations by using various chromatographic methods and structural determination by spectroscopic techniques such as IR, NMR including 2D-NMR and MS. The structures of the compounds were also elucidated by comparison with the previ...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1999
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/9484/1/FSAS_1999_25_A.pdf |
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| Summary: | The study on Murraya koenigii (curry leaf) involves extraction,
separations by using various chromatographic methods and structural
determination by spectroscopic techniques such as IR, NMR including 2D-NMR
and MS. The structures of the compounds were also elucidated by comparison
with the previous work. Isolation works on stem bark of the plant yielded
mahanimbine (31), girinimbine (32), murray anine (47), murrayazoline (48),
murrayacine (49) and sucrose (50). From the leaves, five compounds were
isolated and elucidated. Two of them were long chain compounds, Il-decylhenecosane
(51a) and methoxydotriacontane (51b), isolated as a mixture and the other compounds were mahanimbine (31), ethyl octadecanoate (52) and
mahanine (53).
Further study on the roots of the same plant afforded four compounds
including girinimbine (32), murrayanine (47), together with 3-methylcarbazole
(54) and murrayafoline A(55).Crude extract and isolated compounds from various part of this plant
were screened for antimicrobial activity using disc diffusion method, cytotoxic
activity using rnicrotitration method and antitumor promoting activity using
Epstein B arr virus activation assay. The crude extracts of the roots and pure
isolated compound including mah animbine (31) , girinimbine (32), mahanine
(53) and murrayafoline A (55) exhibited significant cytotoxicity activity against
CEM-SS cell line with IC₅₀ 3 µg/ml. G irinimbine (32), although did not show
any cytotoxic activity against CEM-SS cell line, the compound however
inhibited EBV-activation (100%) in the antitumor promoting assay . The
pet.ether, CHCl₃ and MeOH crude extracts of leaves, the methanol extract of
stem bark, mah animbine (31) and girinimbine (32) failed to show significant
antimicrobial activity, while the pet.ether and the chloroform crude extracts of
the stem bark exhibited weak antibacterial activity against Bacillus cereus. |
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