The Effect of N-Nitrosodimethylamine on Enzyme Activities and Histology in the Mouse

This thesis studied the effects of acute, sub-chronic and chronic exposures of NDMA on the enzyme activities of five tumour markers namely: glutathione Stransferase (GST), gamma glutamyl transpeptidase (y-GT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-R) and uridyl diphosphogluc...

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Main Author:	Salleh, Mohd Nazil
Format:	Thesis
Language:	English English
Published:	1999
Subjects:	Dimethylnitrosamine Tumor markers
Online Access:	http://psasir.upm.edu.my/id/eprint/9496/1/FSAS_1999_33_A.pdf
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spelling	my-upm-ir.94962013-09-26T00:31:05Z The Effect of N-Nitrosodimethylamine on Enzyme Activities and Histology in the Mouse 1999-07 Salleh, Mohd Nazil This thesis studied the effects of acute, sub-chronic and chronic exposures of NDMA on the enzyme activities of five tumour markers namely: glutathione Stransferase (GST), gamma glutamyl transpeptidase (y-GT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-R) and uridyl diphosphoglucuronyl transferase (UDPGT) in the liver and kidney of male mice were. Forty-eight male mice ICR strain, Mus musculus (26-3 8g, 8 weeks old) were divided into four groups. A single batch consisted of 12 mice; one-half of them for enzyme activities and the other one-half for histological study. Acute group (n= 12) were injected with a single dose of 0.5 mg/kg b.w. of NDMA. Sub-chronic group received 0.25 mg/kg b.w. of NDMA with twice i.p. injection over a one month period. Chronic group (n= 12) were injected with 0.05 mg/kg b.w. of NDMA given four times over a period of four month. The mice were sacrificed by cervical dislocation after treatment. In the liver, acute, sub-chronic and chronic exposure to NDMA showed increased enzyme activities (GST, GSH-R, GSH-Px, y-GT and UDPGT: except y-GT at acute exposure) in comparison with control groups (P<0.05). In the kidney, however the effect of NDMA exposure was rather inconsistent. All the enzyme activities increased in the acute exposure. Meanwhile, in the sub-chronic and chronic exposure some enzyme activities increased (e.g.: GST, y-GT and UDPGT) and some enzyme activities (e.g.: GSH-R) did not change significantly. Administration of NDMA to mice by intraperitoneal inj ection over various periods of times to a total of 0.05- 0.5 mg/kg body weight caused the development of the tumour in the liver and kidney. Single acute dose (0.5 mg/kg b.w. of NDMA) produced anaplastic tumour and tubular degeneration in the kidney. Prolonged exposure (chronic) of mice to NDMA produced a high incidence of hepatocellular adenoma and also necrosis in the liver. This study demonstrated that there is a very strong correlation between enzyme activities and cell damage. Therefore, it can be concluded that some enzyme activities especially GST, y-GT and UDPGT can be used as tumour markers. Dimethylnitrosamine Tumor markers 1999-07 Thesis http://psasir.upm.edu.my/id/eprint/9496/ http://psasir.upm.edu.my/id/eprint/9496/1/FSAS_1999_33_A.pdf application/pdf en public masters Universiti Putra Malaysia Dimethylnitrosamine Tumor markers Faculty of Science and Environmental Studies English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Dimethylnitrosamine Tumor markers
spellingShingle	Dimethylnitrosamine Tumor markers Salleh, Mohd Nazil The Effect of N-Nitrosodimethylamine on Enzyme Activities and Histology in the Mouse
description	This thesis studied the effects of acute, sub-chronic and chronic exposures of NDMA on the enzyme activities of five tumour markers namely: glutathione Stransferase (GST), gamma glutamyl transpeptidase (y-GT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-R) and uridyl diphosphoglucuronyl transferase (UDPGT) in the liver and kidney of male mice were. Forty-eight male mice ICR strain, Mus musculus (26-3 8g, 8 weeks old) were divided into four groups. A single batch consisted of 12 mice; one-half of them for enzyme activities and the other one-half for histological study. Acute group (n= 12) were injected with a single dose of 0.5 mg/kg b.w. of NDMA. Sub-chronic group received 0.25 mg/kg b.w. of NDMA with twice i.p. injection over a one month period. Chronic group (n= 12) were injected with 0.05 mg/kg b.w. of NDMA given four times over a period of four month. The mice were sacrificed by cervical dislocation after treatment. In the liver, acute, sub-chronic and chronic exposure to NDMA showed increased enzyme activities (GST, GSH-R, GSH-Px, y-GT and UDPGT: except y-GT at acute exposure) in comparison with control groups (P<0.05). In the kidney, however the effect of NDMA exposure was rather inconsistent. All the enzyme activities increased in the acute exposure. Meanwhile, in the sub-chronic and chronic exposure some enzyme activities increased (e.g.: GST, y-GT and UDPGT) and some enzyme activities (e.g.: GSH-R) did not change significantly. Administration of NDMA to mice by intraperitoneal inj ection over various periods of times to a total of 0.05- 0.5 mg/kg body weight caused the development of the tumour in the liver and kidney. Single acute dose (0.5 mg/kg b.w. of NDMA) produced anaplastic tumour and tubular degeneration in the kidney. Prolonged exposure (chronic) of mice to NDMA produced a high incidence of hepatocellular adenoma and also necrosis in the liver. This study demonstrated that there is a very strong correlation between enzyme activities and cell damage. Therefore, it can be concluded that some enzyme activities especially GST, y-GT and UDPGT can be used as tumour markers.
format	Thesis
qualification_level	Master's degree
author	Salleh, Mohd Nazil
author_facet	Salleh, Mohd Nazil
author_sort	Salleh, Mohd Nazil
title	The Effect of N-Nitrosodimethylamine on Enzyme Activities and Histology in the Mouse
title_short	The Effect of N-Nitrosodimethylamine on Enzyme Activities and Histology in the Mouse
title_full	The Effect of N-Nitrosodimethylamine on Enzyme Activities and Histology in the Mouse
title_fullStr	The Effect of N-Nitrosodimethylamine on Enzyme Activities and Histology in the Mouse
title_full_unstemmed	The Effect of N-Nitrosodimethylamine on Enzyme Activities and Histology in the Mouse
title_sort	effect of n-nitrosodimethylamine on enzyme activities and histology in the mouse
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	1999
url	http://psasir.upm.edu.my/id/eprint/9496/1/FSAS_1999_33_A.pdf
_version_	1747810966617194496

The Effect of N-Nitrosodimethylamine on Enzyme Activities and Histology in the Mouse

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