Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes

Mungbean (subgenus Ceratotropis) is an important food source in many parts of the world, particularly in Asia and Southeast Asia. It is an important source of plant protein and calcium, and is a good substitute for meat. Although it is an important crop, little is known about its genetic backgrou...

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Main Author:	Vijay Kumar
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Microsatellites (Genetics). Genetic markers. Mung bean.
Online Access:	http://psasir.upm.edu.my/id/eprint/9533/1/FSAS_2003_1_A.pdf
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id	my-upm-ir.9533
record_format	uketd_dc
spelling	my-upm-ir.95332012-08-27T05:17:04Z Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes 2003-01 Vijay Kumar, Mungbean (subgenus Ceratotropis) is an important food source in many parts of the world, particularly in Asia and Southeast Asia. It is an important source of plant protein and calcium, and is a good substitute for meat. Although it is an important crop, little is known about its genetic background. DNA markers, in particular micro satellites, are able to provide insights regarding the genetic structure and background of populations and thus would be of great benefit in mungbean improvement programs. Three techniques were used to isolate micro satellite loci in mungbean, namely direct amplification of length polymorphism (DALP), 5' anchored PCR and random hybridizing micro satellites (RAHM). A total of 107 repeat sequences were identified of which 80% were micro satellite loci and 20% were cryptic simple regions. The majority of micro satellites were found using the 5' anchored PCR procedure which proved to be the most efficient technique in the present study, while DALP did not produce any microsatellite. Microsatellites (Genetics). Genetic markers. Mung bean. 2003-01 Thesis http://psasir.upm.edu.my/id/eprint/9533/ http://psasir.upm.edu.my/id/eprint/9533/1/FSAS_2003_1_A.pdf application/pdf en public phd doctoral Universiti Putra Malaysia Microsatellites (Genetics). Genetic markers. Mung bean. Faculty of Science and Environmental Studies English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Microsatellites (Genetics). Genetic markers. Mung bean.
spellingShingle	Microsatellites (Genetics). Genetic markers. Mung bean. Vijay Kumar, Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes
description	Mungbean (subgenus Ceratotropis) is an important food source in many parts of the world, particularly in Asia and Southeast Asia. It is an important source of plant protein and calcium, and is a good substitute for meat. Although it is an important crop, little is known about its genetic background. DNA markers, in particular micro satellites, are able to provide insights regarding the genetic structure and background of populations and thus would be of great benefit in mungbean improvement programs. Three techniques were used to isolate micro satellite loci in mungbean, namely direct amplification of length polymorphism (DALP), 5' anchored PCR and random hybridizing micro satellites (RAHM). A total of 107 repeat sequences were identified of which 80% were micro satellite loci and 20% were cryptic simple regions. The majority of micro satellites were found using the 5' anchored PCR procedure which proved to be the most efficient technique in the present study, while DALP did not produce any microsatellite.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Vijay Kumar,
author_facet	Vijay Kumar,
author_sort	Vijay Kumar,
title	Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes
title_short	Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes
title_full	Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes
title_fullStr	Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes
title_full_unstemmed	Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes
title_sort	isolation, characterization and application of dna microsatellite markers in mungbean (vigna radiata l. wilczek) and other selected legumes
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/9533/1/FSAS_2003_1_A.pdf
_version_	1747810975404261376

Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes

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