Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
Chilli (Capsicum annuum L.) is one of the major vegetable and spice crops grown widely, yet it suffers great losses due to infection by various viruses, including Cucumber Mosaic Virus (CMV). The conventional breeding of chilli hybrids for improved disease resistance is a long process with proble...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2003
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/9538/1/FSAS_2003_7_A.pdf |
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| Summary: | Chilli (Capsicum annuum L.) is one of the major vegetable and spice crops grown
widely, yet it suffers great losses due to infection by various viruses, including
Cucumber Mosaic Virus (CMV). The conventional breeding of chilli hybrids for
improved disease resistance is a long process with problems of interspecific
incompatibility. The objectives of the study were to construct a transforming vector
containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) and development of
an Agrobacterium-transformation procedure which can offer solutions to these
problems.
This study was approached primarily from the molecular aspect which the construct
of a CMV CP has been successfully cloned. The cloned fragments were 655 bp and
exhibited more than 90% similarity to those published CMV CP gene sequences. SDS-PAGE analysis showed the size of the expressed coat protein to be similar to
the predicted size of 30 kD based on the DNA sequences. Western blot analysis was
also conducted to confirm the bacterial expressed coat proteins. All these verified
that the cloned genes were translatable CMV CP genes. |
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