Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli

Chilli (Capsicum annuum L.) is one of the major vegetable and spice crops grown widely, yet it suffers great losses due to infection by various viruses, including Cucumber Mosaic Virus (CMV). The conventional breeding of chilli hybrids for improved disease resistance is a long process with proble...

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Main Author:	Lim, Jee Hian
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Capsicum annuum Cucumber mosaic virus Agrobacterium
Online Access:	http://psasir.upm.edu.my/id/eprint/9538/1/FSAS_2003_7_A.pdf
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id	my-upm-ir.9538
record_format	uketd_dc
spelling	my-upm-ir.95382012-05-21T01:48:51Z Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli 2003-05 Lim, Jee Hian Chilli (Capsicum annuum L.) is one of the major vegetable and spice crops grown widely, yet it suffers great losses due to infection by various viruses, including Cucumber Mosaic Virus (CMV). The conventional breeding of chilli hybrids for improved disease resistance is a long process with problems of interspecific incompatibility. The objectives of the study were to construct a transforming vector containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) and development of an Agrobacterium-transformation procedure which can offer solutions to these problems. This study was approached primarily from the molecular aspect which the construct of a CMV CP has been successfully cloned. The cloned fragments were 655 bp and exhibited more than 90% similarity to those published CMV CP gene sequences. SDS-PAGE analysis showed the size of the expressed coat protein to be similar to the predicted size of 30 kD based on the DNA sequences. Western blot analysis was also conducted to confirm the bacterial expressed coat proteins. All these verified that the cloned genes were translatable CMV CP genes. Capsicum annuum Cucumber mosaic virus Agrobacterium 2003-05 Thesis http://psasir.upm.edu.my/id/eprint/9538/ http://psasir.upm.edu.my/id/eprint/9538/1/FSAS_2003_7_A.pdf application/pdf en public masters Universiti Putra Malaysia Capsicum annuum Cucumber mosaic virus Agrobacterium Faculty of Science and Environmental Studies English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Capsicum annuum Cucumber mosaic virus Agrobacterium
spellingShingle	Capsicum annuum Cucumber mosaic virus Agrobacterium Lim, Jee Hian Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
description	Chilli (Capsicum annuum L.) is one of the major vegetable and spice crops grown widely, yet it suffers great losses due to infection by various viruses, including Cucumber Mosaic Virus (CMV). The conventional breeding of chilli hybrids for improved disease resistance is a long process with problems of interspecific incompatibility. The objectives of the study were to construct a transforming vector containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) and development of an Agrobacterium-transformation procedure which can offer solutions to these problems. This study was approached primarily from the molecular aspect which the construct of a CMV CP has been successfully cloned. The cloned fragments were 655 bp and exhibited more than 90% similarity to those published CMV CP gene sequences. SDS-PAGE analysis showed the size of the expressed coat protein to be similar to the predicted size of 30 kD based on the DNA sequences. Western blot analysis was also conducted to confirm the bacterial expressed coat proteins. All these verified that the cloned genes were translatable CMV CP genes.
format	Thesis
qualification_level	Master's degree
author	Lim, Jee Hian
author_facet	Lim, Jee Hian
author_sort	Lim, Jee Hian
title	Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_short	Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_full	Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_fullStr	Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_full_unstemmed	Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_sort	construction of a transformation vector containing cucumber mosaic virus (cmv) coat protein (cp) gene and development of an agrobacteriumtransformation procedure for chilli
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/9538/1/FSAS_2003_7_A.pdf
_version_	1747810976630046720

Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli

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