Molecular Genetic Characterization of Different Accessions of Centella Asiatica

Centella asiatica or locally known as "pegaga" belongs to the Apiceae family. This medicinal plant is one of the most important medicinal herbs, and widely used in health foods, pharmaceutical and cosmetic industries. Twelve accessions of C. asiatica planted in MARDI, originated from di...

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Main Author:	Wong, Sook Mun
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Molecular genetics - Malaysia Plants - Classification - Molecular aspects - Malaysia
Online Access:	http://psasir.upm.edu.my/id/eprint/9542/1/FSAS_2003_12_A.pdf
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id	my-upm-ir.9542
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spelling	my-upm-ir.95422012-05-21T03:32:29Z Molecular Genetic Characterization of Different Accessions of Centella Asiatica 2003-01 Wong, Sook Mun Centella asiatica or locally known as "pegaga" belongs to the Apiceae family. This medicinal plant is one of the most important medicinal herbs, and widely used in health foods, pharmaceutical and cosmetic industries. Twelve accessions of C. asiatica planted in MARDI, originated from different locations in Peninsular Malaysia, were used for this study. Phenotypic differences among these accessions are not very distinct, thus this study is undeitaken to determine whether there are any genetic differences. DNA markers, unaff ected by environmental or physiological factors, have potential utility in the characterization of plant species. High discriminating power of this class of markers demonstrated uniformity and stability within genetically complex cultivars. Good quality DNA was extracted from leaf samples using conventional hexadecyltrimethylammonium bromide (CTAB) method. Two PeR-based DNA markers system. namely Amplified Fragment Length Polymorphisms (AFLPs) and Long Primer Randomly Amplified Polymorphic DNA (LP-RAPDs), were employed. This study has successfully analyzed the genetic relationships among the accessions of C. asiatica. Two phylogenetic trees bad been constructed from the unweighted pair group method with arithmatical average (UPGMA) pairwise analyses. Genetic distances was calculated based on. the Dice similarity index. From both analyses, the CA01 and CA02 as wen as CA05 and CA06 were closer (D=0.119) within the same cluster indicating that they are closely related. Based on the genetic distances, CA10 represented as highest distant group in the LP-RAPDs analyses whereas CA03 represented as highest distant group in the AFLPs analyses. Furthermore, diagnostic band with highest molecular weight (3000 bp) was found in CA10 by using long primer PEH A3. The amplification of CA03 genotypes with AFLP primer pair ACG/CTA has shown a unique DNA profile. In addition, CA03 is easily distinguish from other accessions morphologically due to its wavy shape of the plant leaf. Molecular genetics - Malaysia Plants - Classification - Molecular aspects - Malaysia 2003-01 Thesis http://psasir.upm.edu.my/id/eprint/9542/ http://psasir.upm.edu.my/id/eprint/9542/1/FSAS_2003_12_A.pdf application/pdf en public masters Universiti Putra Malaysia Molecular genetics - Malaysia Plants - Classification - Molecular aspects - Malaysia Faculty of Science and Environmental Studies English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Molecular genetics - Malaysia Plants - Classification - Molecular aspects - Malaysia
spellingShingle	Molecular genetics - Malaysia Plants - Classification - Molecular aspects - Malaysia Wong, Sook Mun Molecular Genetic Characterization of Different Accessions of Centella Asiatica
description	Centella asiatica or locally known as "pegaga" belongs to the Apiceae family. This medicinal plant is one of the most important medicinal herbs, and widely used in health foods, pharmaceutical and cosmetic industries. Twelve accessions of C. asiatica planted in MARDI, originated from different locations in Peninsular Malaysia, were used for this study. Phenotypic differences among these accessions are not very distinct, thus this study is undeitaken to determine whether there are any genetic differences. DNA markers, unaff ected by environmental or physiological factors, have potential utility in the characterization of plant species. High discriminating power of this class of markers demonstrated uniformity and stability within genetically complex cultivars. Good quality DNA was extracted from leaf samples using conventional hexadecyltrimethylammonium bromide (CTAB) method. Two PeR-based DNA markers system. namely Amplified Fragment Length Polymorphisms (AFLPs) and Long Primer Randomly Amplified Polymorphic DNA (LP-RAPDs), were employed. This study has successfully analyzed the genetic relationships among the accessions of C. asiatica. Two phylogenetic trees bad been constructed from the unweighted pair group method with arithmatical average (UPGMA) pairwise analyses. Genetic distances was calculated based on. the Dice similarity index. From both analyses, the CA01 and CA02 as wen as CA05 and CA06 were closer (D=0.119) within the same cluster indicating that they are closely related. Based on the genetic distances, CA10 represented as highest distant group in the LP-RAPDs analyses whereas CA03 represented as highest distant group in the AFLPs analyses. Furthermore, diagnostic band with highest molecular weight (3000 bp) was found in CA10 by using long primer PEH A3. The amplification of CA03 genotypes with AFLP primer pair ACG/CTA has shown a unique DNA profile. In addition, CA03 is easily distinguish from other accessions morphologically due to its wavy shape of the plant leaf.
format	Thesis
qualification_level	Master's degree
author	Wong, Sook Mun
author_facet	Wong, Sook Mun
author_sort	Wong, Sook Mun
title	Molecular Genetic Characterization of Different Accessions of Centella Asiatica
title_short	Molecular Genetic Characterization of Different Accessions of Centella Asiatica
title_full	Molecular Genetic Characterization of Different Accessions of Centella Asiatica
title_fullStr	Molecular Genetic Characterization of Different Accessions of Centella Asiatica
title_full_unstemmed	Molecular Genetic Characterization of Different Accessions of Centella Asiatica
title_sort	molecular genetic characterization of different accessions of centella asiatica
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/9542/1/FSAS_2003_12_A.pdf
_version_	1747810977603125248

Molecular Genetic Characterization of Different Accessions of Centella Asiatica

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