In Vitro Studies on the Virulence of Spodoptera Litura Baculovirus

Baculoviruses have been used as biopesticides against economic pests in agriculture, forestry and landscapes. In vitro production of baculoviruses has often been considered especially because of the ease of large-scale propagation. In this study, in vitro production was investigated by the infect...

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Main Author:	Mohammed Hussein, Syakira
Format:	Thesis
Language:	English English
Published:	2003
Subjects:	Fertilization in vitro Virulence (Microbiology) - Immunological aspects Baculoviruses
Online Access:	http://psasir.upm.edu.my/id/eprint/9606/1/FSAS_2003_58_IR.pdf
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id	my-upm-ir.9606
record_format	uketd_dc
spelling	my-upm-ir.96062023-11-28T02:21:17Z In Vitro Studies on the Virulence of Spodoptera Litura Baculovirus 2003-12 Mohammed Hussein, Syakira Baculoviruses have been used as biopesticides against economic pests in agriculture, forestry and landscapes. In vitro production of baculoviruses has often been considered especially because of the ease of large-scale propagation. In this study, in vitro production was investigated by the infectivity of two genera of baculoviruses, Nucleopolyhedrosis virus (NPV) and Granulovirus (GV) to cultured lepidopteran cells. Production of baculoviruses depends on the ability of these cells to replicate in an optimal condition. Spodoptera litura baculovirus isolated locally was investigated for its virulence to two cell lines by inoculating with both forms of the virus, occlusion-body derived virions (PDV) and budded virus (BV), from SpltNPV and SpltGV. Efforts to develop cell cultures from the local Spodoptera litura for use in replicating these local baculoviruses was, however, unsuccessful due to cell deterioration or microbial contamination. A cell line from Spodoptera Jrugiperda (S£9) was susceptible to budded virus obtained directly from the insect, but not to viral progeny. While these results suggest Sf9 cells have potential for replicating these baculoviruses, the Spodoptera litura cell line, TUAT -Spli-221 appeared not to be susceptible. Fertilization in vitro Virulence (Microbiology) - Immunological aspects Baculoviruses 2003-12 Thesis http://psasir.upm.edu.my/id/eprint/9606/ http://psasir.upm.edu.my/id/eprint/9606/1/FSAS_2003_58_IR.pdf text en public masters Universiti Putra Malaysia Fertilization in vitro Virulence (Microbiology) - Immunological aspects Baculoviruses Faculty of Science and Environmental Studies Abdul Samad, Norani English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Abdul Samad, Norani
topic	Fertilization in vitro Virulence (Microbiology) - Immunological aspects Baculoviruses
spellingShingle	Fertilization in vitro Virulence (Microbiology) - Immunological aspects Baculoviruses Mohammed Hussein, Syakira In Vitro Studies on the Virulence of Spodoptera Litura Baculovirus
description	Baculoviruses have been used as biopesticides against economic pests in agriculture, forestry and landscapes. In vitro production of baculoviruses has often been considered especially because of the ease of large-scale propagation. In this study, in vitro production was investigated by the infectivity of two genera of baculoviruses, Nucleopolyhedrosis virus (NPV) and Granulovirus (GV) to cultured lepidopteran cells. Production of baculoviruses depends on the ability of these cells to replicate in an optimal condition. Spodoptera litura baculovirus isolated locally was investigated for its virulence to two cell lines by inoculating with both forms of the virus, occlusion-body derived virions (PDV) and budded virus (BV), from SpltNPV and SpltGV. Efforts to develop cell cultures from the local Spodoptera litura for use in replicating these local baculoviruses was, however, unsuccessful due to cell deterioration or microbial contamination. A cell line from Spodoptera Jrugiperda (S£9) was susceptible to budded virus obtained directly from the insect, but not to viral progeny. While these results suggest Sf9 cells have potential for replicating these baculoviruses, the Spodoptera litura cell line, TUAT -Spli-221 appeared not to be susceptible.
format	Thesis
qualification_level	Master's degree
author	Mohammed Hussein, Syakira
author_facet	Mohammed Hussein, Syakira
author_sort	Mohammed Hussein, Syakira
title	In Vitro Studies on the Virulence of Spodoptera Litura Baculovirus
title_short	In Vitro Studies on the Virulence of Spodoptera Litura Baculovirus
title_full	In Vitro Studies on the Virulence of Spodoptera Litura Baculovirus
title_fullStr	In Vitro Studies on the Virulence of Spodoptera Litura Baculovirus
title_full_unstemmed	In Vitro Studies on the Virulence of Spodoptera Litura Baculovirus
title_sort	in vitro studies on the virulence of spodoptera litura baculovirus
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Science and Environmental Studies
publishDate	2003
url	http://psasir.upm.edu.my/id/eprint/9606/1/FSAS_2003_58_IR.pdf
_version_	1794018854024773632

In Vitro Studies on the Virulence of Spodoptera Litura Baculovirus

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