In Vitro Culture of Muskmelon - Cucumis Melo Var. Birdie
Several explants from Fl hybrid seeds and aseptically-germinated seedlings of muskmelon Cucumis melo var. Birdie, were tested for their capacity for plant regeneration through direct organogenesis in vitro. Excised cotyledons and - embryos from imbibed, ungerminated seeds were cultured on Mura...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1989
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/9683/1/FSMB_1989_1_A.pdf |
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| Summary: | Several explants from Fl hybrid seeds and
aseptically-germinated seedlings of muskmelon Cucumis
melo var. Birdie, were tested for their capacity for
plant regeneration through direct organogenesis in
vitro.
Excised cotyledons and - embryos from imbibed,
ungerminated seeds were cultured on Murashige and Skoog
(MS) medium containing 1.0 - 5.0 uM and 1.0-50.0 uM benzylaminopurine (BAP), kinetin, or isopentenyl adenine
(2iP) respectively, in the presence or absence of 5.0 uM
gibberellic acid (GA₃). Embryo explants produced
callus on all media tested, and only 39 of cotyledon
explants produced buds on medium containing 2.0 - 3.0 uM
BAP.
Unimbibed, testaless seeds were germinated
aseptically on MS medium in the presence of 1.0 - 50.0
uM BAP, kinetin or 2 ip. On medium containing 3.0 uM
BAP, 68% of cultures produced small buds in the region
of the cotyledonary node within three weeks. The
addition of GAl (5.0 uM) or naphthalene acetic acid
(NAA) (0.1 - 1.5 uM) did not increase the percentage of
cultures which produced buds.
Cotyledonary nodes were subcultured to media
containing the same (3.0 UK) or lower concentrations of
BAP for two weeks and then to MS basal medium for
another two weeks to allow further bud development and
shoot elongation. The highest number of shoots per
cotyl edonary node (9.7) was obtained when the first
subculture medium contained 0.5 uM BAP.
Individual shoots less than 1.0 cm, 1.0 - 2.5 cm
and more than 2.5 cm in length, were tested for rooting ability in the presence of 3.0 uM IBA. The highest
percentage rooting (49) occurred in shoots more than
1.0cm long. Further studies on root induction showed
that the highest percentage of rooting (85% ), occurred
when shoots 1.0 - 2.5 cm lonq were cultured in the
light, on filter-paper bridqes in liquid, half-strength
MS medium containing 2.0 uM NAA , without charcoal.
Complete plantlets. fNere successfully transplanted
to the qlasshouse and qrown in hydroponic culture. The
plants flowered and produced normal fruit. |
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