Growth, leaf gas exchange and biochemical changes of oil palm (Elaeis guineensis Jacq.) seedlings as affected by iron oxide nanoparticles

Iron oxide nanoparticles (Fe3O4 NPs) has a great potential to boost up plant growth. However, depending on the size, concentration and the plant type used, controversial results have been obtained. Therefore, their fate in the plant body should be investigated to lower the negative impacts and raisi...

Full description

Saved in:

Bibliographic Details
Main Author:	Izad, Ayu Azera
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Oil-palm - Biochemistry Seeds Biochemistry
Online Access:	http://psasir.upm.edu.my/id/eprint/97626/1/FS%202021%2035%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.97626
record_format	uketd_dc
spelling	my-upm-ir.976262022-06-09T04:11:59Z Growth, leaf gas exchange and biochemical changes of oil palm (Elaeis guineensis Jacq.) seedlings as affected by iron oxide nanoparticles 2021-02 Izad, Ayu Azera Iron oxide nanoparticles (Fe3O4 NPs) has a great potential to boost up plant growth. However, depending on the size, concentration and the plant type used, controversial results have been obtained. Therefore, their fate in the plant body should be investigated to lower the negative impacts and raising the beneficial impacts of the Fe3O4 NPs utilization on oil palm, major crop cultivated in Malaysia. Thus, the objectives of this study are 1) to investigate the impact of different Fe3O4 NPs concentrations i.e. (0, 800, 1600 and 2400 mg/L) on the growth, leaf gas exchange and biochemical changes of 3- months old oil palm seedlings and; 2) to study the physiological adaptation (growth, leaf gas exchange and biochemical properties) and uptake of Fe3O4 NPs by the 3-months old oil palm seedlings when exposed to low Fe3O4 NPs concentrations, i.e. (0, 200, 400 and 600 mg/L). Both experiments were arranged in a randomized complete block design (RCBD) and replicated three times. The first experiment revealed that the oil palm seedlings unable to tolerate even the lowest concentration of Fe3O4 NPs (800 mg/L). The plant growth was not significantly affected by Fe3O4 NPs, but it significantly (p ≤ 0.05) reduced the SPAD chlorophyll value and the leaf total stomata density as compared to the control. Besides, the net photosynthesis is significantly reduced due to damage of photosynthetic apparatus of Fe3O4 NPs-stressed seedlings, in comparison to control. Moreover, the production of malondialdehyde (MDA) is positively correlated with the total volume of phenolics and total flavonoids. This observation indicates that an increase in MDA might be responsible for the up-regulation of the secondary metabolites production under high Fe3O4 NPs concentration application. Meanwhile, in the second experiment, the long-term exposure of low Fe3O4 NPs concentration application significantly reduced the plant height, total biomass, basal diameter, leaf number, total leaf area, and relative growth rate. Leaf gas exchange and chlorophyll fluorescence characteristics of treated seedlings decreased under high Fe3O4 NPs application, compared to the control. Electrolyte leakage (EL) and leaf respiration rate were gradually increased as the Fe3O4 NPs application elevated up to 600 mg/L. Proline, total phenolics, and iron content were significantly increased with Fe3O4 NPs application. The increasing magnetic signal of electron spin resonance (ESR) spectra confirmed the existence of Fe3O4 NPs in root cells of the treatment plants. In conclusion, the uptake of Fe3O4 NPs at 200 to 2400 mg/L concentration dramatically reduced the oil palm seedlings growth as well as photosynthesis efficiency and production of secondary metabolites were elevated as physiological adaptation responses of oil palm seedlings to withstand the impact of Fe3O4 NPs application. Oil-palm - Biochemistry Seeds Biochemistry 2021-02 Thesis http://psasir.upm.edu.my/id/eprint/97626/ http://psasir.upm.edu.my/id/eprint/97626/1/FS%202021%2035%20IR.pdf text en public masters Universiti Putra Malaysia Oil-palm - Biochemistry Seeds Biochemistry Ibrahim, Mohd Hafiz
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Ibrahim, Mohd Hafiz
topic	Oil-palm - Biochemistry Seeds Biochemistry
spellingShingle	Oil-palm - Biochemistry Seeds Biochemistry Izad, Ayu Azera Growth, leaf gas exchange and biochemical changes of oil palm (Elaeis guineensis Jacq.) seedlings as affected by iron oxide nanoparticles
description	Iron oxide nanoparticles (Fe3O4 NPs) has a great potential to boost up plant growth. However, depending on the size, concentration and the plant type used, controversial results have been obtained. Therefore, their fate in the plant body should be investigated to lower the negative impacts and raising the beneficial impacts of the Fe3O4 NPs utilization on oil palm, major crop cultivated in Malaysia. Thus, the objectives of this study are 1) to investigate the impact of different Fe3O4 NPs concentrations i.e. (0, 800, 1600 and 2400 mg/L) on the growth, leaf gas exchange and biochemical changes of 3- months old oil palm seedlings and; 2) to study the physiological adaptation (growth, leaf gas exchange and biochemical properties) and uptake of Fe3O4 NPs by the 3-months old oil palm seedlings when exposed to low Fe3O4 NPs concentrations, i.e. (0, 200, 400 and 600 mg/L). Both experiments were arranged in a randomized complete block design (RCBD) and replicated three times. The first experiment revealed that the oil palm seedlings unable to tolerate even the lowest concentration of Fe3O4 NPs (800 mg/L). The plant growth was not significantly affected by Fe3O4 NPs, but it significantly (p ≤ 0.05) reduced the SPAD chlorophyll value and the leaf total stomata density as compared to the control. Besides, the net photosynthesis is significantly reduced due to damage of photosynthetic apparatus of Fe3O4 NPs-stressed seedlings, in comparison to control. Moreover, the production of malondialdehyde (MDA) is positively correlated with the total volume of phenolics and total flavonoids. This observation indicates that an increase in MDA might be responsible for the up-regulation of the secondary metabolites production under high Fe3O4 NPs concentration application. Meanwhile, in the second experiment, the long-term exposure of low Fe3O4 NPs concentration application significantly reduced the plant height, total biomass, basal diameter, leaf number, total leaf area, and relative growth rate. Leaf gas exchange and chlorophyll fluorescence characteristics of treated seedlings decreased under high Fe3O4 NPs application, compared to the control. Electrolyte leakage (EL) and leaf respiration rate were gradually increased as the Fe3O4 NPs application elevated up to 600 mg/L. Proline, total phenolics, and iron content were significantly increased with Fe3O4 NPs application. The increasing magnetic signal of electron spin resonance (ESR) spectra confirmed the existence of Fe3O4 NPs in root cells of the treatment plants. In conclusion, the uptake of Fe3O4 NPs at 200 to 2400 mg/L concentration dramatically reduced the oil palm seedlings growth as well as photosynthesis efficiency and production of secondary metabolites were elevated as physiological adaptation responses of oil palm seedlings to withstand the impact of Fe3O4 NPs application.
format	Thesis
qualification_level	Master's degree
author	Izad, Ayu Azera
author_facet	Izad, Ayu Azera
author_sort	Izad, Ayu Azera
title	Growth, leaf gas exchange and biochemical changes of oil palm (Elaeis guineensis Jacq.) seedlings as affected by iron oxide nanoparticles
title_short	Growth, leaf gas exchange and biochemical changes of oil palm (Elaeis guineensis Jacq.) seedlings as affected by iron oxide nanoparticles
title_full	Growth, leaf gas exchange and biochemical changes of oil palm (Elaeis guineensis Jacq.) seedlings as affected by iron oxide nanoparticles
title_fullStr	Growth, leaf gas exchange and biochemical changes of oil palm (Elaeis guineensis Jacq.) seedlings as affected by iron oxide nanoparticles
title_full_unstemmed	Growth, leaf gas exchange and biochemical changes of oil palm (Elaeis guineensis Jacq.) seedlings as affected by iron oxide nanoparticles
title_sort	growth, leaf gas exchange and biochemical changes of oil palm (elaeis guineensis jacq.) seedlings as affected by iron oxide nanoparticles
granting_institution	Universiti Putra Malaysia
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/97626/1/FS%202021%2035%20IR.pdf
_version_	1747813792908050432

Growth, leaf gas exchange and biochemical changes of oil palm (Elaeis guineensis Jacq.) seedlings as affected by iron oxide nanoparticles

Similar Items