Fabrication of chitosan-integrated single-mode tapered optical fiber DENV II E protein sensor
Globally, diseases infected by dengue virus (DENV) prevails among major public health problem, especially in tropical and sub-tropical areas. 500 000 people are estimated infected with severe dengue require hospitalization every year and 2.5% is estimated case fatality. Quantitative assessment...
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2020
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Abu Bakar, Muhammad Hafiz |
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Chitosan - Research Nanocomposites (Materials) Dengue - Analysis |
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Chitosan - Research Nanocomposites (Materials) Dengue - Analysis Mohd Amin @ Mohd Nasir, Nadia Fabrication of chitosan-integrated single-mode tapered optical fiber DENV II E protein sensor |
| description |
Globally, diseases infected by dengue virus (DENV) prevails among major public
health problem, especially in tropical and sub-tropical areas. 500 000 people are
estimated infected with severe dengue require hospitalization every year and
2.5% is estimated case fatality. Quantitative assessment by enzyme-linked
immunosorbent assay (ELISA) is known to be used by laboratories to produce
better clinical monitoring but it needs complex laboratories infrastructure as well
as expertise to operate it. For the past decades, tapered single mode fiber has
shown versatility and enticing sensitivity towards changes in its surrounding
refractive index, making it suitable for sensing applications. In 2018, a research
developing tapered optical fiber sensor targeting dengue virus envelope (E)
protein originates from DENV II which is among 4 distinct DENV serotypes has
been published. DENV II E protein is the interested determinant since it is
located at the outermost of dengue virus structure, hence detecting the protein
signifies the presence of the virus itself. As a result, high sensitivity and
specificity within rapid detection 15 minutes is achieved. This biosensor is
enhanced further by utilizing inorganic material which is graphene to facilitate
greater surface area for sensing enhancement. However, graphene is known to
have mild toxicity and its effect to DENV II E protein is yet to be determined.
This study looks into the use of organic nanomaterial namely chitosan for
enhancement of tapered fiber sensing response. A layer of chitosan was
introduced to single mode tapered fiber functionalized for the detection of DENV
II E protein. Tapered optical fiber was fabricated and functionalized using
Sodium Hydroxide (NaOH), 3-(Aminopropyl) triethoxysilane (APTES), and
Glutaraldehyde. Chitosan immersion time up to 60 minutes was then tested yielding working immersion time of 20 (CHIT20), 30 (CHIT30), 35 (CHIT35), 40
(CHIT40) and 45 minutes (CHIT45). Subsequently, the experiment proceeded
with the immobilization of antibody. The immersion time for antibody was
optimized for CHIT20, CHIT30, CHIT35, CHIT40 and CHIT45 at 25, 30, 33, 35
and 38 minutes, respectively.
After that, different concentration of DENV II E protein solution ranging from
0.0nM to 1.0nM with increment of 0.2nM were introduced. Prior to that, optimum
incubation time of DENV II E protein for CHIT20, CHIT30, CHIT35, CHIT40 and
CHIT45 was observed at 30, 35, 38, 40 and 43 minutes respectively. The
spectral shift with the introduction of DENV II E protein was then recorded and
analyzed. This set of experiment was conducted in triplicates. Consistent red
shift of spectra at increasing concentration is observed for CHIT20. It obeys the
linear relationship between concentration and refractive index which altered the
effective refractive index and caused the red shift. For CHIT30, CHIT35, CHIT40
and CHIT45, consistent red shift of spectra was also noted. Increment of the
sensitivity value is observed as CHIT20, CHIT30 and CHIT35 recorded 6.28
nm/nM, 10.68 nm/nM and 14.19 nm/nM, respectively. However, the sensitivity
decreased for CHIT40 and CHIT45 with corresponding value of 12.24 nm/nM
and 11.34 nm/nM. From these values, it is noted that the best sensitivity
obtained for the sensor is at CHIT35 with 14.19 nm/nM. The work proceeded
with the investigation on limit of detection (LOD) and the sensor was tested with
different concentration ranging from 0.1pM to 0.1µM. The Langmuir curve plotted
from the findings denoted LOD of 1pM. In conclusion, this study highlights the
feasibility of using organic nanomaterial which has better biocompatibility and
environmental friendly for the enhancement of DENV II E protein detection. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Mohd Amin @ Mohd Nasir, Nadia |
| author_facet |
Mohd Amin @ Mohd Nasir, Nadia |
| author_sort |
Mohd Amin @ Mohd Nasir, Nadia |
| title |
Fabrication of chitosan-integrated single-mode tapered optical fiber DENV II E protein sensor |
| title_short |
Fabrication of chitosan-integrated single-mode tapered optical fiber DENV II E protein sensor |
| title_full |
Fabrication of chitosan-integrated single-mode tapered optical fiber DENV II E protein sensor |
| title_fullStr |
Fabrication of chitosan-integrated single-mode tapered optical fiber DENV II E protein sensor |
| title_full_unstemmed |
Fabrication of chitosan-integrated single-mode tapered optical fiber DENV II E protein sensor |
| title_sort |
fabrication of chitosan-integrated single-mode tapered optical fiber denv ii e protein sensor |
| granting_institution |
Universiti Putra Malaysia |
| publishDate |
2020 |
| url |
http://psasir.upm.edu.my/id/eprint/97785/1/FK%202021%201%20UPM%20IR.pdf |
| _version_ |
1747813805485719552 |
| spelling |
my-upm-ir.977852022-06-24T02:35:43Z Fabrication of chitosan-integrated single-mode tapered optical fiber DENV II E protein sensor 2020-01 Mohd Amin @ Mohd Nasir, Nadia Globally, diseases infected by dengue virus (DENV) prevails among major public health problem, especially in tropical and sub-tropical areas. 500 000 people are estimated infected with severe dengue require hospitalization every year and 2.5% is estimated case fatality. Quantitative assessment by enzyme-linked immunosorbent assay (ELISA) is known to be used by laboratories to produce better clinical monitoring but it needs complex laboratories infrastructure as well as expertise to operate it. For the past decades, tapered single mode fiber has shown versatility and enticing sensitivity towards changes in its surrounding refractive index, making it suitable for sensing applications. In 2018, a research developing tapered optical fiber sensor targeting dengue virus envelope (E) protein originates from DENV II which is among 4 distinct DENV serotypes has been published. DENV II E protein is the interested determinant since it is located at the outermost of dengue virus structure, hence detecting the protein signifies the presence of the virus itself. As a result, high sensitivity and specificity within rapid detection 15 minutes is achieved. This biosensor is enhanced further by utilizing inorganic material which is graphene to facilitate greater surface area for sensing enhancement. However, graphene is known to have mild toxicity and its effect to DENV II E protein is yet to be determined. This study looks into the use of organic nanomaterial namely chitosan for enhancement of tapered fiber sensing response. A layer of chitosan was introduced to single mode tapered fiber functionalized for the detection of DENV II E protein. Tapered optical fiber was fabricated and functionalized using Sodium Hydroxide (NaOH), 3-(Aminopropyl) triethoxysilane (APTES), and Glutaraldehyde. Chitosan immersion time up to 60 minutes was then tested yielding working immersion time of 20 (CHIT20), 30 (CHIT30), 35 (CHIT35), 40 (CHIT40) and 45 minutes (CHIT45). Subsequently, the experiment proceeded with the immobilization of antibody. The immersion time for antibody was optimized for CHIT20, CHIT30, CHIT35, CHIT40 and CHIT45 at 25, 30, 33, 35 and 38 minutes, respectively. After that, different concentration of DENV II E protein solution ranging from 0.0nM to 1.0nM with increment of 0.2nM were introduced. Prior to that, optimum incubation time of DENV II E protein for CHIT20, CHIT30, CHIT35, CHIT40 and CHIT45 was observed at 30, 35, 38, 40 and 43 minutes respectively. The spectral shift with the introduction of DENV II E protein was then recorded and analyzed. This set of experiment was conducted in triplicates. Consistent red shift of spectra at increasing concentration is observed for CHIT20. It obeys the linear relationship between concentration and refractive index which altered the effective refractive index and caused the red shift. For CHIT30, CHIT35, CHIT40 and CHIT45, consistent red shift of spectra was also noted. Increment of the sensitivity value is observed as CHIT20, CHIT30 and CHIT35 recorded 6.28 nm/nM, 10.68 nm/nM and 14.19 nm/nM, respectively. However, the sensitivity decreased for CHIT40 and CHIT45 with corresponding value of 12.24 nm/nM and 11.34 nm/nM. From these values, it is noted that the best sensitivity obtained for the sensor is at CHIT35 with 14.19 nm/nM. The work proceeded with the investigation on limit of detection (LOD) and the sensor was tested with different concentration ranging from 0.1pM to 0.1µM. The Langmuir curve plotted from the findings denoted LOD of 1pM. In conclusion, this study highlights the feasibility of using organic nanomaterial which has better biocompatibility and environmental friendly for the enhancement of DENV II E protein detection. Chitosan - Research Nanocomposites (Materials) Dengue - Analysis 2020-01 Thesis http://psasir.upm.edu.my/id/eprint/97785/ http://psasir.upm.edu.my/id/eprint/97785/1/FK%202021%201%20UPM%20IR.pdf text en public masters Universiti Putra Malaysia Chitosan - Research Nanocomposites (Materials) Dengue - Analysis Abu Bakar, Muhammad Hafiz |