Characterisation of bovine serum albumin-based biomaterials synthesised by subcritical water technology
Bovine serum albumin (BSA), is one of the most abundant proteins in mammals’ blood, especially from bovine and human. This study investigated the synthesis and characterization of BSA-based biomaterials that was produced by subcritical water technology (SCWT). BSA is freely available and is a che...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/97812/1/FK%202020%2095%20-%20IR.1.pdf |
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| Summary: | Bovine serum albumin (BSA), is one of the most abundant proteins in mammals’
blood, especially from bovine and human. This study investigated the synthesis and
characterization of BSA-based biomaterials that was produced by subcritical water
technology (SCWT). BSA is freely available and is a cheap source of protein to be
recycled into various biomaterials. Using toxin and non-environmentally friendly
chemicals is the main drawback of the current methods of biomaterials processes as
well as long procedures time. Therefore, the primary goal of this study was to assess
whether Subcritical Water can be used as a cheap, clean and simple alternative to the
traditional recovery method to produce biomaterials from BSA. In addition, this is the
first study ever analyzing ‘BSA’ biomaterials employing the simple and quick SCWT
method without using any catalyst or chemicals. Although BSA solution
decomposition at high SCWT temperature (above 2500C) and long SCWT holding
time (5 mins and longer) was investigated wisely, we focused on low SCWT
temperature (below 2500C) which provided indications that it could deliver valuable
products as its high temperature treatment. The best conditions of processing
biomaterials were identified by assessing the effect of different influential parameters
such as SCWT temperature, reaction time, and initial BSA concentration. SCWT was
carried out using a batch tube reactor to produce BSA-based biomaterials (BSA-based
hydrogel and BSA-based nanoparticles). BSA molecules in temperature range of 80-
1200C accumulate to BSA-based hydrogel. It was found that at SCWT temperature
more than 1300C, due to the water/ethanol mixture behavior of SCW, in very specific
SCWT condition, accumulated BSA molecules decomposed to BSA-based
nanoparticles and following by decomposing to organic components. The behavior of
BSA-based hydrogel was investigated in different media wisely which confirmed that
it has an excellent ability to use as drug delivery. By characterization of the BSAbased
nanoparticles and liquid-phase components produced were conducted. In short, the result of this study showed that SCWT of BSA is the best method in terms
of time, efficiency and quality of produced biomaterials as well as the method’s nondependence
on catalyst or toxic chemicals. Low, medium and high SCWT temperature
of BSA (80-2000C) was found to contain beneficial biomaterials useful for medical,
especially drug delivery application. |
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