Screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in Malaysia

Animals under the family Felidae both domesticated and in the wild are potentially harbouring viruses of importance to other animals and humans. Canine Parvovirus (CPV) is a single stranded DNA virus which known to cause severe disease in younger unvaccinated animals. Despite the widespread vaccinat...

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Main Author:	Ahmad Nadzri, Nur Farahiyah
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Parvoviruses Feline immunodeficiency virus infection Tiger
Online Access:	http://psasir.upm.edu.my/id/eprint/97992/1/FPV%202019%2025%20IR.pdf
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id	my-upm-ir.97992
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spelling	my-upm-ir.979922022-07-14T02:23:26Z Screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in Malaysia 2018-03 Ahmad Nadzri, Nur Farahiyah Animals under the family Felidae both domesticated and in the wild are potentially harbouring viruses of importance to other animals and humans. Canine Parvovirus (CPV) is a single stranded DNA virus which known to cause severe disease in younger unvaccinated animals. Despite the widespread vaccination of domestic carnivores, CPV is still an important pathogen of domestic and wild carnivores. In addition, CPV able to infect a wide range of feline hosts, hence, allowing it to infect both cats and dogs. Although, CPV is well studied in canine, limited information is available on it occurrence in various species of felids found in Malaysia. This study investigated detection of viruses of potential importance in different species of felines namely leopards, feral cats and tigers in Malaysia based on virus isolation in Crandell Rees feline kidney (CRFK) cells, polymerase chain reaction (PCR) using gene-specific primers and sequence analysis. From a total of 36 samples collected, 11 samples showed cytopathic effect in cell culture and were subjected to PCR using specific primers for feline herpesvirus (FHV), feline calicivirus (FCV), canine distemper virus (CDV) and CPV. However, only one sample from a tiger was detected positive for canine parvovirus (CPV). The entire viral genome of the sample CPV/MY/MT4 was amplified by PCR for sequencing using Sanger sequencing approach. Genome sequencing of the isolate revealed substitution of amino acid which characterized the isolate as variant CPV namely new CPV-2a with a characteristic’s amino acid substitution at position 297 of VP2 gene from serine to alanine (297-Ser-Ala). Additionally, at amino acid residue 426, isolate CPV/MY/MT4 have amino acid substitution aspartic acid to asparagine (Asp-426-Asn), which also revealed that the isolate has specific amino acid for CPV-2a. In addition, CPV/MY/MT4 was found to be phylogenetically close to new CPV-2a strain from other countries namely USA and Japan. The rest of the other new CPV-2a strains had distinct lineage but shared molecular relationship with the isolate CPV/MY/MT4. In conclusion, genome sequencing of the isolated CPV provided valuable information of the molecular characteristic of the isolate for further study on the importance of this virus in tigers and species of felids. Parvoviruses Feline immunodeficiency virus infection Tiger 2018-03 Thesis http://psasir.upm.edu.my/id/eprint/97992/ http://psasir.upm.edu.my/id/eprint/97992/1/FPV%202019%2025%20IR.pdf text en public masters Universiti Putra Malaysia Parvoviruses Feline immunodeficiency virus infection Tiger Omar, Abdul Rahman
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Omar, Abdul Rahman
topic	Parvoviruses Feline immunodeficiency virus infection Tiger
spellingShingle	Parvoviruses Feline immunodeficiency virus infection Tiger Ahmad Nadzri, Nur Farahiyah Screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in Malaysia
description	Animals under the family Felidae both domesticated and in the wild are potentially harbouring viruses of importance to other animals and humans. Canine Parvovirus (CPV) is a single stranded DNA virus which known to cause severe disease in younger unvaccinated animals. Despite the widespread vaccination of domestic carnivores, CPV is still an important pathogen of domestic and wild carnivores. In addition, CPV able to infect a wide range of feline hosts, hence, allowing it to infect both cats and dogs. Although, CPV is well studied in canine, limited information is available on it occurrence in various species of felids found in Malaysia. This study investigated detection of viruses of potential importance in different species of felines namely leopards, feral cats and tigers in Malaysia based on virus isolation in Crandell Rees feline kidney (CRFK) cells, polymerase chain reaction (PCR) using gene-specific primers and sequence analysis. From a total of 36 samples collected, 11 samples showed cytopathic effect in cell culture and were subjected to PCR using specific primers for feline herpesvirus (FHV), feline calicivirus (FCV), canine distemper virus (CDV) and CPV. However, only one sample from a tiger was detected positive for canine parvovirus (CPV). The entire viral genome of the sample CPV/MY/MT4 was amplified by PCR for sequencing using Sanger sequencing approach. Genome sequencing of the isolate revealed substitution of amino acid which characterized the isolate as variant CPV namely new CPV-2a with a characteristic’s amino acid substitution at position 297 of VP2 gene from serine to alanine (297-Ser-Ala). Additionally, at amino acid residue 426, isolate CPV/MY/MT4 have amino acid substitution aspartic acid to asparagine (Asp-426-Asn), which also revealed that the isolate has specific amino acid for CPV-2a. In addition, CPV/MY/MT4 was found to be phylogenetically close to new CPV-2a strain from other countries namely USA and Japan. The rest of the other new CPV-2a strains had distinct lineage but shared molecular relationship with the isolate CPV/MY/MT4. In conclusion, genome sequencing of the isolated CPV provided valuable information of the molecular characteristic of the isolate for further study on the importance of this virus in tigers and species of felids.
format	Thesis
qualification_level	Master's degree
author	Ahmad Nadzri, Nur Farahiyah
author_facet	Ahmad Nadzri, Nur Farahiyah
author_sort	Ahmad Nadzri, Nur Farahiyah
title	Screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in Malaysia
title_short	Screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in Malaysia
title_full	Screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in Malaysia
title_fullStr	Screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in Malaysia
title_full_unstemmed	Screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in Malaysia
title_sort	screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in malaysia
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/97992/1/FPV%202019%2025%20IR.pdf
_version_	1747813826848358400

Screening of important viruses in felines and molecular characterization of parvovirus isolate from a tiger in Malaysia

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