Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia

The emergence of the Fusarium wilt of banana (Panama disease) caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Fusarium odoratissimum) which caused loss to the global banana production is known to infect majority banana cultivar. A total of 38 isolates of Fusarium spp. related to b...

Full description

Saved in:

Bibliographic Details
Main Author:	Ujat, Anysia Hedy
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Fusarium wilt of banana Bananas - Diseases and pests
Online Access:	http://psasir.upm.edu.my/id/eprint/98594/1/FP%202021%2048%20UPMIR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.98594
record_format	uketd_dc
spelling	my-upm-ir.985942022-09-06T07:03:20Z Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia 2021-02 Ujat, Anysia Hedy The emergence of the Fusarium wilt of banana (Panama disease) caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Fusarium odoratissimum) which caused loss to the global banana production is known to infect majority banana cultivar. A total of 38 isolates of Fusarium spp. related to banana Fusarium wilt obtained from the Biological Control Laboratory, Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia were cultured on different media for observation of culture characteristics. Based on the culture morphology, isolates are grouped into four different group and a representative isolate were chosen for each group, namely Prk 4-1, Ns 5-2, Phg 3 and Swk 4. Micromorphology shows no significant difference in the macro- and microconidia size. To further identify the isolates, molecular analysis was conducted by using the sequences of Histone H3 gene and TEF-1α gene. TR4 specific primers were used to confirm the presence of Fusarium odoratissimum (TR4). The phylogenetic tree using Histone H3 sequences showed all isolates grouped into Fusarium oxysporum species complex while phylogenetic tree using TEF-1α sequences revealed that the samples were composed of 27 isolates of Fusarium odoratissimum (TR4), two isolate Fusarium grosmichelii (R1), six unknown Fusarium oxysporum species complexes isolates and three Fusarium fujikuroi isolates. By using TR4 specific primers, all 27 samples were confirmed to be Fusarium odoratissimum (TR4). To study the susceptibility of local banana cultivar, pathogenicity test was conducted on 3 months old banana plantlets consisting of five different local banana cultivars of three different genome types: Musa acuminata AAA (Pisang Berangan and Pisang Serendah), Musa paradisiaca AAB (Pisang Nangka and Pisang Raja) and Musa balbisiana BBB (Pisang Nipah). Observation was recorded for 30 days, and foliar symptoms showed different severity of disease progression. However, internal symptoms showed that all of the inoculated plantlets were severely infected. Cooking banana (AAB and BBB) of local cultivar were also found to be susceptible to Fusarium odoratissimum (TR4). This study shows that there are not only Fusarium odoratissimum (TR4) presence in Malaysia but also Fusarium grosmichelii (R1) and a possibility of an unknown strain of Fusarium oxysporum. Pathogenicity test reveals that local banana cultivar in Malaysia are susceptible to Fusarium wilt disease regardless of the genotype of the banana plants. These findings will aid in the substantive documentation of Fusarium spp. in the local banana plantation scenario. Fusarium wilt of banana Bananas - Diseases and pests 2021-02 Thesis http://psasir.upm.edu.my/id/eprint/98594/ http://psasir.upm.edu.my/id/eprint/98594/1/FP%202021%2048%20UPMIR.pdf text en public masters Universiti Putra Malaysia Fusarium wilt of banana Bananas - Diseases and pests Mohamed Zulperi, Dzarifah
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Mohamed Zulperi, Dzarifah
topic	Fusarium wilt of banana Bananas - Diseases and pests
spellingShingle	Fusarium wilt of banana Bananas - Diseases and pests Ujat, Anysia Hedy Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
description	The emergence of the Fusarium wilt of banana (Panama disease) caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Fusarium odoratissimum) which caused loss to the global banana production is known to infect majority banana cultivar. A total of 38 isolates of Fusarium spp. related to banana Fusarium wilt obtained from the Biological Control Laboratory, Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia were cultured on different media for observation of culture characteristics. Based on the culture morphology, isolates are grouped into four different group and a representative isolate were chosen for each group, namely Prk 4-1, Ns 5-2, Phg 3 and Swk 4. Micromorphology shows no significant difference in the macro- and microconidia size. To further identify the isolates, molecular analysis was conducted by using the sequences of Histone H3 gene and TEF-1α gene. TR4 specific primers were used to confirm the presence of Fusarium odoratissimum (TR4). The phylogenetic tree using Histone H3 sequences showed all isolates grouped into Fusarium oxysporum species complex while phylogenetic tree using TEF-1α sequences revealed that the samples were composed of 27 isolates of Fusarium odoratissimum (TR4), two isolate Fusarium grosmichelii (R1), six unknown Fusarium oxysporum species complexes isolates and three Fusarium fujikuroi isolates. By using TR4 specific primers, all 27 samples were confirmed to be Fusarium odoratissimum (TR4). To study the susceptibility of local banana cultivar, pathogenicity test was conducted on 3 months old banana plantlets consisting of five different local banana cultivars of three different genome types: Musa acuminata AAA (Pisang Berangan and Pisang Serendah), Musa paradisiaca AAB (Pisang Nangka and Pisang Raja) and Musa balbisiana BBB (Pisang Nipah). Observation was recorded for 30 days, and foliar symptoms showed different severity of disease progression. However, internal symptoms showed that all of the inoculated plantlets were severely infected. Cooking banana (AAB and BBB) of local cultivar were also found to be susceptible to Fusarium odoratissimum (TR4). This study shows that there are not only Fusarium odoratissimum (TR4) presence in Malaysia but also Fusarium grosmichelii (R1) and a possibility of an unknown strain of Fusarium oxysporum. Pathogenicity test reveals that local banana cultivar in Malaysia are susceptible to Fusarium wilt disease regardless of the genotype of the banana plants. These findings will aid in the substantive documentation of Fusarium spp. in the local banana plantation scenario.
format	Thesis
qualification_level	Master's degree
author	Ujat, Anysia Hedy
author_facet	Ujat, Anysia Hedy
author_sort	Ujat, Anysia Hedy
title	Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_short	Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_full	Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_fullStr	Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_full_unstemmed	Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_sort	characterization and pathogenicity of fusarium species complex associated with fusarium wilt of banana in malaysia
granting_institution	Universiti Putra Malaysia
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/98594/1/FP%202021%2048%20UPMIR.pdf
_version_	1747813880604655616

Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia

Similar Items