Population genetic structures of Eleutheronema tetradactylum (Shaw, 1804) and Eleutheronema rhadinum (Jordan and Evermann, 1902) in Malaysian waters

The four finger threadfin, Eleutheronema tetradactylum (Shaw, 1804) and East Asian four finger threadfin, Eleutheronema rhadinum (Jordan and Everman, 1902) are two species of the Polynemidae family that can be found in Malaysian waters. Common name among local is Senangin. There have been no popu...

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Main Author:	Wahid, Nurul Atikah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Fishes - Genetics Polynemidae
Online Access:	http://psasir.upm.edu.my/id/eprint/99175/1/FP%202020%2043%20IR.pdf
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id	my-upm-ir.99175
record_format	uketd_dc
spelling	my-upm-ir.991752023-03-06T04:24:33Z Population genetic structures of Eleutheronema tetradactylum (Shaw, 1804) and Eleutheronema rhadinum (Jordan and Evermann, 1902) in Malaysian waters 2019-11 Wahid, Nurul Atikah The four finger threadfin, Eleutheronema tetradactylum (Shaw, 1804) and East Asian four finger threadfin, Eleutheronema rhadinum (Jordan and Everman, 1902) are two species of the Polynemidae family that can be found in Malaysian waters. Common name among local is Senangin. There have been no population genetic studies in both species in Malaysia, which are important to provide genetic information for sustainable fisheries management of the two species. Moreover, both Senangin species are also difficult to distinguish morphologically, thus their taxonomic classification are not fully resolved. Therefore, the aims for this study were to determine the levels of genetic divergences between the two Senangin fishes, and to quantify their population genetic structure by using both mitochondrial DNA and microsatellite markers. Totally 151 samples of Senangin were collected from six location around Malaysian coastal water. Samples were obtained from commercial fishing activities. Among 151 individuals, 20 distinct haplotypes were defined. The phylogenetic tree (maximum likelihood) showed two genealogical branches were recognized among the haplotype, this also proven in pairwise distance among the haplotypes. Level of genetic divergence was substantially high (15-17%) supported their taxonomic status as distinct species. The nucleotide diversity was low in E. tetradactylum and E. rhadinum. Both species show the sharing of haplotypes between different populations and this support that both species had a historically widespread natural distribution in the region in the past. Microsatellites analyses for E. tetradactylum also indicated low levels of genetic variation and high degree of population structure among the E. tetradactylum populations. The average observed heterozygosity (HO = 0.5191) obtained was lower than the standard heterozygosity found in most marine populations (HO = 0.79). Through assignment test all samples were assigned to their respective populations. The low genetic variation in both of mitochondrial DNA and microsatellite proved the evidence of over-exploitation of E. tetradactylum in Malaysia. In conclusion, the present study was able to differentiate between E. tetradactylum and E. rhadinum using mitochondrial gene, Cytochrome Oxidase I. The levels of genetic differentiation and population structure between the two species were successfully quantified. Fishes - Genetics Polynemidae 2019-11 Thesis http://psasir.upm.edu.my/id/eprint/99175/ http://psasir.upm.edu.my/id/eprint/99175/1/FP%202020%2043%20IR.pdf text en public masters Universiti Putra Malaysia Fishes - Genetics Polynemidae Esa, Yuzine
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Esa, Yuzine
topic	Fishes - Genetics Polynemidae
spellingShingle	Fishes - Genetics Polynemidae Wahid, Nurul Atikah Population genetic structures of Eleutheronema tetradactylum (Shaw, 1804) and Eleutheronema rhadinum (Jordan and Evermann, 1902) in Malaysian waters
description	The four finger threadfin, Eleutheronema tetradactylum (Shaw, 1804) and East Asian four finger threadfin, Eleutheronema rhadinum (Jordan and Everman, 1902) are two species of the Polynemidae family that can be found in Malaysian waters. Common name among local is Senangin. There have been no population genetic studies in both species in Malaysia, which are important to provide genetic information for sustainable fisheries management of the two species. Moreover, both Senangin species are also difficult to distinguish morphologically, thus their taxonomic classification are not fully resolved. Therefore, the aims for this study were to determine the levels of genetic divergences between the two Senangin fishes, and to quantify their population genetic structure by using both mitochondrial DNA and microsatellite markers. Totally 151 samples of Senangin were collected from six location around Malaysian coastal water. Samples were obtained from commercial fishing activities. Among 151 individuals, 20 distinct haplotypes were defined. The phylogenetic tree (maximum likelihood) showed two genealogical branches were recognized among the haplotype, this also proven in pairwise distance among the haplotypes. Level of genetic divergence was substantially high (15-17%) supported their taxonomic status as distinct species. The nucleotide diversity was low in E. tetradactylum and E. rhadinum. Both species show the sharing of haplotypes between different populations and this support that both species had a historically widespread natural distribution in the region in the past. Microsatellites analyses for E. tetradactylum also indicated low levels of genetic variation and high degree of population structure among the E. tetradactylum populations. The average observed heterozygosity (HO = 0.5191) obtained was lower than the standard heterozygosity found in most marine populations (HO = 0.79). Through assignment test all samples were assigned to their respective populations. The low genetic variation in both of mitochondrial DNA and microsatellite proved the evidence of over-exploitation of E. tetradactylum in Malaysia. In conclusion, the present study was able to differentiate between E. tetradactylum and E. rhadinum using mitochondrial gene, Cytochrome Oxidase I. The levels of genetic differentiation and population structure between the two species were successfully quantified.
format	Thesis
qualification_level	Master's degree
author	Wahid, Nurul Atikah
author_facet	Wahid, Nurul Atikah
author_sort	Wahid, Nurul Atikah
title	Population genetic structures of Eleutheronema tetradactylum (Shaw, 1804) and Eleutheronema rhadinum (Jordan and Evermann, 1902) in Malaysian waters
title_short	Population genetic structures of Eleutheronema tetradactylum (Shaw, 1804) and Eleutheronema rhadinum (Jordan and Evermann, 1902) in Malaysian waters
title_full	Population genetic structures of Eleutheronema tetradactylum (Shaw, 1804) and Eleutheronema rhadinum (Jordan and Evermann, 1902) in Malaysian waters
title_fullStr	Population genetic structures of Eleutheronema tetradactylum (Shaw, 1804) and Eleutheronema rhadinum (Jordan and Evermann, 1902) in Malaysian waters
title_full_unstemmed	Population genetic structures of Eleutheronema tetradactylum (Shaw, 1804) and Eleutheronema rhadinum (Jordan and Evermann, 1902) in Malaysian waters
title_sort	population genetic structures of eleutheronema tetradactylum (shaw, 1804) and eleutheronema rhadinum (jordan and evermann, 1902) in malaysian waters
granting_institution	Universiti Putra Malaysia
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/99175/1/FP%202020%2043%20IR.pdf
_version_	1776100298068066304

Population genetic structures of Eleutheronema tetradactylum (Shaw, 1804) and Eleutheronema rhadinum (Jordan and Evermann, 1902) in Malaysian waters

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