Antiproliferative activities of methanolic extract of Chromolaena odorata (L.) R.M. king & H.Rob in a mice model
Chromolaena odorata and Melastoma malabathricum are plants that normally found in wasteland in Malaysia and both have great value in wound healing. Previous literatures reported that these plants possess antiproliferative, antibacterial and antiinflammatory effects. This study was conducted to in...
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| Format: | Thesis |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/99208/1/FPV%202020%2018%20%20IR.pdf |
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| Summary: | Chromolaena odorata and Melastoma malabathricum are plants that normally found
in wasteland in Malaysia and both have great value in wound healing. Previous
literatures reported that these plants possess antiproliferative, antibacterial and antiinflammatory
effects. This study was conducted to investigate the antiproliferative
activity of the methanolic extract of C.odorata (MeCO) in a mice model. The
antiproliferative effect was conducted by MTT assay and the results revealed that
MeCO has higher antiproliferative effect with IC50 value 78 μg/ml compared with M.
malabathricum (no IC50 value). Thus, MeCO was chosen for in vivo experiment. For
acute oral toxicity study, mice were divided into two groups, control and treatment
(5000 mg/kg of MeCO). Results showed that there was no significant different of mice
administered with MeCO in body weight. However, food intake of treatment mice was
significant different (p < 0.05) compared to control group. There was significant
decrease (p < 0.05) of lung weight in treatment group compared to control group.
However, there was no significant difference in relative organ weight of liver, kidney
and spleen of the treatment group compared to control group. Besides, the serum
biochemical analysis showed that there was no significant different of mice
administered with plant extract compared to control except alkaline phosphatase
(ALP). ALP was significantly lower (p < 0.05) compared to control group.
Histological examination showed normal architecture for spleen and kidney in both
groups. However, mild congestion observed in liver and lungs of treatment group. For
efficacy study, the mice were divided into six groups (n = 6) which were 250 mg/kg
MeCO, 500 mg/kg MeCO, 1000 mg/kg MeCO, control (normal), untreated control
mice and vehicle control. The mean survival time of the treatment groups was
significantly higher (p < 0.05) compared to untreated control group. The liver weight
of untreated control, 250 mg/kg and 500 mg/kg was significantly higher (p < 0.05)
compared normal group. Besides, the tumour weight of mice treated with 1000 mg/kg
was significantly lower (p < 0.05) compared to untreated control group. Serum
biochemical analysis showed that the glucose was significantly different (p < 0.05) in all groups compared to the normal while urea level in vehicle control was significantly
lower (p < 0.05) compared to normal. Histopathological examination resulted in
metastasis of the mammary tumour to various organs such as spleen, liver, lungs in all
groups. In conclusion, the MeCO extract has a potential of antiproliferative agent in
breast cancer in vitro and in vivo. |
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