Genetic Diversity of Acacia Crassicarpa A. Cunn. Ex Benth.Plus Trees of a Provenance Trial in Serdang, Malaysia
In the selection of the best species for forest plantation, few criterias have to be considered including morphological and genetic diversity. Thus, the objective of this study was to investigate the diversity of Acacia crassicarpa plus trees using morphological and genetic markers. The genetic s...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1999
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/9970/1/FH_1999_2_A.pdf |
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| Summary: | In the selection of the best species for forest plantation, few criterias have
to be considered including morphological and genetic diversity. Thus, the
objective of this study was to investigate the diversity of Acacia crassicarpa plus
trees using morphological and genetic markers. The genetic structure and mating
system of the species were also studied.
Plus trees were selected from a provenance trial in Universiti Putra
Malaysia (UPM), Serdang based on 8 qualitative and 2 quantitative parameters
from 2 regions i.e. Queensland (8 plus trees) and Papua New Guinea (23 plus
trees). Leaf samples were collected from these trees and were analysed using 20
isozyme and 15 RAPD markers.
The morphological study observed higher variation and better growth
perfonnance of trees from Papua New Guinea. However, trees from Queensland
have higher retention towards strong wind contradictory to trees from Papua
New Guinea. The isozyme analysis observed 36 loci with 24 loci being
polymorphic. The mean expected heterozygosities were 0.2316 and 0.2675 for
Queensland and Papua New Guinea respectively. The proportions of
polymorphic loci for both regions were found to be similar. In the RAPD
analysis, a total of 8710ci were scored ranging from 0.10 kb to more than 2.10
kb. Generally, provenances from Papua New Guinea were found to produce
higher polymorphism levels as compared to the Queensland provenances. Life
history and the ecological characteristics of the species were believed to be the
possible reasons for such conditions. Cluster analyses produced three different
dendrogram patterns with a tendency of similarity to a certain. The effect of
different approaches was suggested to have caused these differences.
Relationships of clusters according to number of parent trees, altitudes and
longitudes were also observed.
The species was found to be highly outcrossing with rates ranging from
0.69 and 0.94. Genetic differentiation in the species observed 60 to 70% of the
total diversity to be within provenances. Factors such as reproductive biology,
seed dispersal, history and gene flow were suggested to be some of the possible
causes for such phenomena. |
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