Local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation.

Over the few past decades, rapid growth of palm trees has brought a significant impact on economic development in Malaysia. However, many parts of local palms are underutilized as scarce data available on the nutrient profiles of palm trees in Malaysia. In this study, edible core from the palm trees...

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Main Author:	Chai, Lee Ling
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Prebiotics Gastrointestinal system Microbiology
Online Access:	http://psasir.upm.edu.my/id/eprint/99728/1/FSPP%202021%204.pdf
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spelling	my-upm-ir.997282023-04-05T23:54:58Z Local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation. 2021-02 Chai, Lee Ling Over the few past decades, rapid growth of palm trees has brought a significant impact on economic development in Malaysia. However, many parts of local palms are underutilized as scarce data available on the nutrient profiles of palm trees in Malaysia. In this study, edible core from the palm trees cultivated in Sarawak, Malaysia, notably the oil palm (Elaeis guineensis), sago palm (Metroxylon sagu) and coconut (Cocos nucifera) were investigated for their nutritive constituents and prebiotic potential. Inulin, which is a well-established prebiotic, was used as positive control. The raw and cooked palm hearts were prepared for a sequential gastrointestinal system model to study their stability in the simulated saliva, gastric and intestinal conditions. Meanwhile, the pHcontrolled batch culture system was used to delineate the fermentation properties on the colonic microbiota. Samples were obtained at 0, 6th, 12th and 24th hour for bacterial enumeration by fluorescent in situ hybridisation (FISH) and short-chain fatty acid (SCFA) analyses. Among the tested samples, sago palm hearts showed significant highest carbohydrates content (66.81 %) and significant highest potassium content (66 %). All the samples which are partially resistant to the gastrointestinal enzymatic reactions induced similar selective effects towards beneficial bacteria populations as inulin did at the end of fermentation. The highest significant decrease (p ≤ 0.05) in Clostridium histolyticum group populations was observed in the response of raw sago palm hearts at the 24th hour. In general, all the tested substrates produced significantly higher total SCFAs (p ≤ 0.05) than inulin at the 24th hour with the highest production by sago palm heart which was used as raw (286.18 mM) and cooked (284.83 mM). Overall, these findings suggested that raw local palm heart species induced a selective effect towards human gut microbiota and stimulated production of SCFA, indicating potential prebiotic ingredients. Prebiotics Gastrointestinal system Microbiology 2021-02 Thesis http://psasir.upm.edu.my/id/eprint/99728/ http://psasir.upm.edu.my/id/eprint/99728/1/FSPP%202021%204.pdf text en public masters Universiti Putra Malaysia Prebiotics Gastrointestinal system Microbiology
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
topic	Prebiotics Gastrointestinal system Microbiology
spellingShingle	Prebiotics Gastrointestinal system Microbiology Chai, Lee Ling Local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation.
description	Over the few past decades, rapid growth of palm trees has brought a significant impact on economic development in Malaysia. However, many parts of local palms are underutilized as scarce data available on the nutrient profiles of palm trees in Malaysia. In this study, edible core from the palm trees cultivated in Sarawak, Malaysia, notably the oil palm (Elaeis guineensis), sago palm (Metroxylon sagu) and coconut (Cocos nucifera) were investigated for their nutritive constituents and prebiotic potential. Inulin, which is a well-established prebiotic, was used as positive control. The raw and cooked palm hearts were prepared for a sequential gastrointestinal system model to study their stability in the simulated saliva, gastric and intestinal conditions. Meanwhile, the pHcontrolled batch culture system was used to delineate the fermentation properties on the colonic microbiota. Samples were obtained at 0, 6th, 12th and 24th hour for bacterial enumeration by fluorescent in situ hybridisation (FISH) and short-chain fatty acid (SCFA) analyses. Among the tested samples, sago palm hearts showed significant highest carbohydrates content (66.81 %) and significant highest potassium content (66 %). All the samples which are partially resistant to the gastrointestinal enzymatic reactions induced similar selective effects towards beneficial bacteria populations as inulin did at the end of fermentation. The highest significant decrease (p ≤ 0.05) in Clostridium histolyticum group populations was observed in the response of raw sago palm hearts at the 24th hour. In general, all the tested substrates produced significantly higher total SCFAs (p ≤ 0.05) than inulin at the 24th hour with the highest production by sago palm heart which was used as raw (286.18 mM) and cooked (284.83 mM). Overall, these findings suggested that raw local palm heart species induced a selective effect towards human gut microbiota and stimulated production of SCFA, indicating potential prebiotic ingredients.
format	Thesis
qualification_level	Master's degree
author	Chai, Lee Ling
author_facet	Chai, Lee Ling
author_sort	Chai, Lee Ling
title	Local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation.
title_short	Local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation.
title_full	Local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation.
title_fullStr	Local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation.
title_full_unstemmed	Local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation.
title_sort	local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation.
granting_institution	Universiti Putra Malaysia
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/99728/1/FSPP%202021%204.pdf
_version_	1776100355848798208

Local palm hearts (umbut) as potential prebiotic ingredients using in vitro colon model experimentation.

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