Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species
The ability of bacteria to adhere to the surfaces and to form biofilms has major implications in a variety of industries including food industry. The resistance of bacteria to the most of the antibiotics has driven many, researcher to look for alternative natural products. In this work biosurfactan...
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| Format: | Thesis |
| Language: | en_US |
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| Summary: | The ability of bacteria to adhere to the surfaces and to form biofilms has major implications in a variety of industries including food industry. The resistance of bacteria to the most of the antibiotics has driven many, researcher to look for alternative natural products. In this work biosurfactant characteristics. the antimicrobial and anti-biofilm activities of lactic acid bacteria (LAB) were evaluated. The supernatants of LAB in MRS broth were screened for biosurfactants properties using several tests namely haemolysis,oil spreading, drop-collapse, surface tension reduction and emulsification activity tests. LA4 showed the widest hemolytic activity with analysis zone of >3 mm2. ,All of the isolates except LA7, LA8 and LA I0 showed positive droplet collapse test, high percentage of'emulsitication index (E2.1> 50%),and surface tension reduction test. LA 1had the highest emulsification index (E24>100%), surface tension reduction of 37.14#0.49 mN/m. Addition of LAB supernatants to microtiter plates inhibited the growth of P. fluorescence by 50.3-66.9%. P. aeruginosa 14T28 by 29.3-36.3% and P. aeruginosa ATCC27853 by 21.5-26.9%. Percentage biofilm inhibition vary with LAB: LA1 inhibited biofilm P.florescence by 66% LA 13 inhibited biofilm P. aeruginose 14T38 by 36.3% arid P. aeruginosa ATCC27853 by 26.9% after 48h incubation. |
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