Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species

The ability of bacteria to adhere to the surfaces and to form biofilms has major implications in a variety of industries including food industry. The resistance of bacteria to the most of the antibiotics has driven many, researcher to look for alternative natural products. In this work biosurfactan...

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Main Author:	Rasha Sami Bataineh
Format:	Thesis
Language:	en_US
Subjects:	Lactic acid bacteria biosurfactant antimicrobial food industry
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id	my-usim-ddms-12309
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spelling	my-usim-ddms-123092024-05-29T04:00:52Z Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species Rasha Sami Bataineh The ability of bacteria to adhere to the surfaces and to form biofilms has major implications in a variety of industries including food industry. The resistance of bacteria to the most of the antibiotics has driven many, researcher to look for alternative natural products. In this work biosurfactant characteristics. the antimicrobial and anti-biofilm activities of lactic acid bacteria (LAB) were evaluated. The supernatants of LAB in MRS broth were screened for biosurfactants properties using several tests namely haemolysis,oil spreading, drop-collapse, surface tension reduction and emulsification activity tests. LA4 showed the widest hemolytic activity with analysis zone of >3 mm2. ,All of the isolates except LA7, LA8 and LA I0 showed positive droplet collapse test, high percentage of'emulsitication index (E2.1> 50%),and surface tension reduction test. LA 1had the highest emulsification index (E24>100%), surface tension reduction of 37.14#0.49 mN/m. Addition of LAB supernatants to microtiter plates inhibited the growth of P. fluorescence by 50.3-66.9%. P. aeruginosa 14T28 by 29.3-36.3% and P. aeruginosa ATCC27853 by 21.5-26.9%. Percentage biofilm inhibition vary with LAB: LA1 inhibited biofilm P.florescence by 66% LA 13 inhibited biofilm P. aeruginose 14T38 by 36.3% arid P. aeruginosa ATCC27853 by 26.9% after 48h incubation. Universiti Sains Islam Malaysia 2014-02 Thesis en_US https://oarep.usim.edu.my/handle/123456789/12309 https://oarep.usim.edu.my/bitstreams/a4fddb72-8a47-4a7f-a67b-f74dd54f2598/download 8a4605be74aa9ea9d79846c1fba20a33 Lactic acid bacteria biosurfactant antimicrobial food industry
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	en_US
topic	Lactic acid bacteria biosurfactant antimicrobial food industry
spellingShingle	Lactic acid bacteria biosurfactant antimicrobial food industry Rasha Sami Bataineh Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species
description	The ability of bacteria to adhere to the surfaces and to form biofilms has major implications in a variety of industries including food industry. The resistance of bacteria to the most of the antibiotics has driven many, researcher to look for alternative natural products. In this work biosurfactant characteristics. the antimicrobial and anti-biofilm activities of lactic acid bacteria (LAB) were evaluated. The supernatants of LAB in MRS broth were screened for biosurfactants properties using several tests namely haemolysis,oil spreading, drop-collapse, surface tension reduction and emulsification activity tests. LA4 showed the widest hemolytic activity with analysis zone of >3 mm2. ,All of the isolates except LA7, LA8 and LA I0 showed positive droplet collapse test, high percentage of'emulsitication index (E2.1> 50%),and surface tension reduction test. LA 1had the highest emulsification index (E24>100%), surface tension reduction of 37.14#0.49 mN/m. Addition of LAB supernatants to microtiter plates inhibited the growth of P. fluorescence by 50.3-66.9%. P. aeruginosa 14T28 by 29.3-36.3% and P. aeruginosa ATCC27853 by 21.5-26.9%. Percentage biofilm inhibition vary with LAB: LA1 inhibited biofilm P.florescence by 66% LA 13 inhibited biofilm P. aeruginose 14T38 by 36.3% arid P. aeruginosa ATCC27853 by 26.9% after 48h incubation.
format	Thesis
author	Rasha Sami Bataineh
author_facet	Rasha Sami Bataineh
author_sort	Rasha Sami Bataineh
title	Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species
title_short	Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species
title_full	Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species
title_fullStr	Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species
title_full_unstemmed	Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species
title_sort	biosurfactant, antimicrobial and antiadhesion properties of lactic acid bacteria supernatant against pseudomonas species
granting_institution	Universiti Sains Islam Malaysia
_version_	1812444710420086784

Biosurfactant, Antimicrobial and Antiadhesion Properties of Lactic Acid Bacteria Supernatant against Pseudomonas Species

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