Physicochemical Properties And Antimicrobial Activity Of Honey From The Malaysian Stingless Bee Against Selected Pathogenic Bacteria
This study evaluate the physiochemical and antimicrobial activity of stingless bee honey (SBH) or "madu kelulut" produced by five Malaysian stingless bee species (Trigona spp., sample A; Hetrotringona laer'iceap, sample B; Heterotringona thorucica, sample C; Hetero-tringona itama,...
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| Summary: | This study evaluate the physiochemical and antimicrobial activity of stingless bee
honey (SBH) or "madu kelulut" produced by five Malaysian stingless bee species
(Trigona spp., sample A; Hetrotringona laer'iceap, sample B; Heterotringona
thorucica, sample C; Hetero-tringona itama, sample D; and Heirotringonu
terminata, sample E) against Grain negative and positive bacteria namely,
Escherichiu coli ATCC 25922, Bacillus subtilis ATCC 21332, Serrutia murcesccrrs
ATCC 13880, Staphylococcus aureus ATCC 25923, Salmonella Typhimurium ATCC
13 31 1 and Protcus t, ulgaris ATCC 8427. The moisture content of SBH varied
between 29.04 and 37.07%, whereas a%%ra nged between 0.280 for sample A and
0.821 for sample C. Most of the SBH are within the range of yellow shades and amber
with total soluble solids (°Brix) of 75.6+ 7 for sample E and 88.6+6 for sample A. The
antimicrobial activity was evaluated using well diffusion and microtiter plate assay.
The antibacterial activity of SBH varied with bee species and the target bacteria. At
50 % (w/v) of honey sample C and D inhibited all the bacteria; sample A did not
inhibit E. coli; Sample C, D and sample E failed to inhibit the growth of S.
Typhimurium. The pH of raw SBH samples ranged from pH 2.95+0.2 to 3.57+0 with
slight reduction in pH after dilution from 10 to 50%. This study has demonstrated that
Malaysian stingless bee honey has antimicrobial properties against the selected microorganism,
and the low pH of SBH may be responsible for the antimicrobial activity. |
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